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2.5.1.21: squalene synthase

This is an abbreviated version!
For detailed information about squalene synthase, go to the full flat file.

Word Map on EC 2.5.1.21

Reaction

2 (2E,6E)-farnesyl diphosphate +

NAD(P)H
+
H+
=
squalene
 + 2 diphosphate +
NAD(P)+

Synonyms

BbSS, BSS, CrSQS, dt-ySQase, Erg9, EtSS, farnesyl-diphosphate farnesyltransferase, farnesyl-diphosphate:farnesyldiphosphate farnesyltransferase, farnesyldiphosphate farnesyltransferase 1, farnesyldiphosphate:farnesyldiphosphate farnesyltransferase, farnesyltransferase, FDFT1, hSQS, presqualene synthase, presqualene-diphosphate synthase, SgSQS, SQase, SQS, SQS1, SQS2, squalene synthase, squalene synthase 1, squalene synthase 2, squalene synthetase, SS1, SSase, SSN, synthase, squalene, TkSQS1, TkSQS2

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.21 squalene synthase

General Stability

General Stability on EC 2.5.1.21 - squalene synthase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
a combination of 10% methanol, 10% glycerol, 30 mM octyl-beta-D-glucopyranoside, 0.4% Brij-58, and 1 mM DTT in 25 mM sodium phosphate, pH 7.4, essential for stability and maximal activity
-
detergent, e.g. deoxycholate, destabilizes
-
markedly stabilized by 30% glycerol and 0.02 M 2-mercaptoethanol
-
methanol stabilizes
-
methanol, 10%, does not stabilize
NADPH has no effect on stability
-
octylglucoside, 5 mM, causes rapid loss of activity
-
stable in buffer containing 5 mM octylglucoside and 15 mM CHAPS
-
sucrose stabilizes
-
sucrose, 10%, does not stabilize