2.5.1.2: thiamine pyridinylase

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For detailed information about thiamine pyridinylase, go to the full flat file.

Reaction

Synonyms

pyrimidine transferase, thiamin hydrolase, thiamin pyridinolase, thiamin pyridinylase, thiaminase, thiaminase I, thiamine pyridinolase, thiamine pyridinylase

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.2 thiamine pyridinylase

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Results	in table
50	Activating Compound
111	AA Sequence
13	Application
1	CAS Registry Number
2	Cloned(Commentary)
2	Crystallization (Commentary)
21	General Information
62	Inhibitors
8	kcat/KM [mM/s]
2	Ki Value [mM]
62	KM Value [mM]
12	Localization
2	Metals/Ions
9	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
121	Organism
1	Oxidation Stability
2	Pathway
24	PDB ID
19	pH Optimum
5	pH Range
9	pH Stability
1	pI Value
7	Protein Variants
9	Purification (Commentary)
1	Reaction
1	Reaction Type
117	Reference
130	Source Tissue
4	Specific Activity [micromol/min/mg]
105	Substrates and Products (Substrate)
3	Subunits
28	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
17	Temperature Range [°C]
10	Turnover Number [1/s]

Purification

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Purification on EC 2.5.1.2 - thiamine pyridinylase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Paenibacillus thiaminolyticus

P45741

684401

ammonium precipitation and gel filtration

Paenibacillus thiaminolyticus

-

636857

by ammonium precipitation, CM-cellulose column, DEAE-cellulose column, Cibacron blue Sepharose column, Sephacryl S-300 column, Superdex-200 and Superdex-75 column

Cyprinus carpio

-

636860

by ammonium precipitation, DEAE column, Cibacron blue dye affinity column

Paenibacillus thiaminolyticus

P45741

636853

by ammonium precipitation, Sephadex G-25 column, Sephadex G-100 column and DEAE-Sephadex column

Paenibacillus thiaminolyticus

-

636846

by DEAE-cellulose chromatrography, ultrogel AcA 34 column, Sephadex G-100 column and phosphocellulose column

Marsilea drummondii

-

636851

HisTrap FF column chromatography

Paenibacillus thiaminolyticus

-

711655

pI 5.7 isoenzyme

Fistularia petimba

-

699702

Tris-glycine polyacrylamide electrophoresis

Paenibacillus thiaminolyticus

-

636850