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2.5.1.18: glutathione transferase

This is an abbreviated version!
For detailed information about glutathione transferase, go to the full flat file.

Word Map on EC 2.5.1.18

Reaction

RX
+
glutathione
=
HX
 +
R-S-glutathione

Synonyms

2-hydroxychromene-2-carboxylic acid isomerase, 26GST, adGSTD4-4, agGSTe2, allergen Der p 2, allergen Der p 8, alpha class glutathione transferase, Alpha class GST, alpha GST, alpha-class glutathione transferase, asGST5.5, AtGSTF2, AtuGSTH1-1, BaeAB, beta-etherase, bmGSTD, BphK, CgGSTM1, CgGSTM2, cGST, Chi class GST, Chi GST, class mu glutathione S-transferase, delta class glutathione transferase, delta-class glutathione S-transferase, Delta-class glutathione transferase, EC 1.8.6.1, EC 2.5.1.12, EC 2.5.1.13, EC 2.5.1.14, EC 4.4.1.7, EGST, epsilon class GST, Epsilon glutathione S-transferase, Epsilon glutathione transferase, Epsilon GST, epsilon-class glutathione S-transferase, epsilon-class glutathione transferase, GHR, glutathione S-alkyl transferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase, glutathione S-transferase 2, glutathione S-transferase A1-1, glutathione S-transferase A3-3, glutathione S-transferase AdFSTD3-3, glutathione S-transferase I, glutathione S-transferase omega 1, glutathione S-transferase omega 2, glutathione S-transferase P1-1, glutathione S-transferase pi, glutathione S-transferase Pi-1, glutathione S-transferase X, glutathione transferase, glutathione transferase A1-1, glutathione transferase A4-4, glutathione transferase M1-1, glutathione transferase M2-2, glutathione transferase Omega 3S, glutathione transferase omega-1, glutathione transferase P1-1, glutathione transferase Pi, glutathione transferase T1-1, glutathione transferase zeta, glutathione transferase zeta 1, glutathione transferase Zeta 1-1, glutathione transferase zeta1-1, glutathione transferase-like protein, glutathione-S-transferas, glutathione-S-transferase, glutathione-S-transferase pi, glutathione-transferase, GmGSTU4-4, GSH S-transferase, GSH transferase, GSH transferase homologue, GSH-S transferase rho, GSHTase-P, GST, GST A1-1, GST A2-2, GST A3-3, GST A4-4, GST adgstD4-4, GST alpha, GST Delta 2, GST I, GST II, GST III, GST IV, GST M1-1, GST M2-2, GST M4-4, GST M5-5, GST mu, GST O1-1, GST P1-1, GST pi, GST T1-1, GST Tau, GST Tau19, GST Z1-1, GST-1, GST-2, GST-26, GST-3, GST-Acr, GST-OCX-32, GST-T, GST-theta, GST1, GST1b, GST2, GST20, GST3, GST4, GST5, GST5118, GST7, GST83044, Gsta, GSTA1, GSTA1-1, GSTA2-2, GSTA3, GSTA3-3, GSTA4, GSTA4-4, GSTA4L, GSTA5, GSTA5-5, GSTalpha1, GSTD, GSTD1, GSTD10, GSTd14, GSTD2, GSTD4, GSTD4-4, GSTE1, GSTE2, GSTE3, GSTE4, GSTE5, GSTE6, GSTE7, GSTE8, GSTF12-1, GSTF12-2, GSTF3, GSTF5, GSTF9, GSTFuA1, GSTk, GSTK1, GSTL1, GSTL2, GSTL3, Gstm, GSTm09, GSTM1, GSTM1-1, GSTM2, GSTM2-2, GSTM3, GSTM3-3, GSTM4, GSTM4-4, GSTM5, GSTM5-5, GSTmu, GSTO, GSTO1, GSTO1-1, GSTO2, GSTO2-2, GstO2A, GstO2B, GSTO3, GSTO3S, GSTO4, GSTO7, GSTP, GSTP 1-1, GSTP-1, GSTP1, GSTP1-1, Gstr1, GSTrho, GSTS, GSTS1, GSTS1-1, GSTS2, GSTS3, GSTT, GSTT1, GSTT1-1, Gstt1a, GSTT2, GSTT2-2, GSTT2B-2B, GSTT4, GSTT4L, GSTU1, GSTU10, GSTU10-10, GSTU11, GSTU12, GSTU13, GSTU13-1, GSTU14, GSTU16, GSTU17, GSTU18, GSTU19, GSTU2, GSTU2-2, GSTU21, GSTU24, GSTU24-1, GSTU25, GSTU26, GSTU28, GSTU3, GSTU4, GSTU45, GSTU5, GSTU6, GSTU7, GSTU8, GSTU9, GSTZ, Gstz1, GSTZ1-1, GTT1.2, HCCA isomerase, Hematopoietic prostaglandin D synthase, hGSTA-3, hGSTA1-1, hGSTZ1-1, kappa class glutathione transferase, kappa class GSH transferase, Kappa class GST, KKSG9, lambda glutathione transferase, MAAI, MGST1, MGST2, MGST3, Mgst3a, Mgst3b, microsomal glutathione transferase 1, microsomal glutathione transferase-1, More, mtMGST1, mu class glutathione S-transferase, Mu class GST, mu glutathione transferase, Mu GST, mu-class glutathione S-transferase, mu-class glutathione S-transferase1, Mu-class GST, Mu-GST, nu-class glutathione transferase, Omega class GST, omega glutathione S-transferase, omega glutathione transferase, omega-class glutathione S-transferase, omega-class glutathione S-transferase 2, pGSTA1, phi class glutathione transferase, Pi class GST, pm-GSTR1, PmGST, PtGSTU1, PvGSTF1-1, PvGSTU2-2, PvGSTU3-3, rho class glutathione S-transferase, rho-GST, RX: glutathione R-transferase, RX:glutathione R-transferase, S-(hydroxyalkyl)glutathione lyase, Saro_2873/Saro_2872, selenium-containing glutathione transferase zeta1-1, seleno-hGSTZ1-1, SGST26.5, sigma class glutathione transferase, Sigma class GST, sigma glutathione S-transferase, sigma-class glutathione S-transferase, sigma-class GST, SIGST, Sj26GST, sjGST, sll1545, ssGST3, ssGST5, tau class glutathione S-transferase, tau class glutathione transferase, tau class GST, tau class GSTU4-4, TDR1, theta class glutathione S-transferase, theta class glutathione transferase T1-1, theta class GST, theta-class glutathione transferase, thiol-dependent reductase I, Ure2p mutant A122C, Ure2pB1, Xi class glutathione transferase, Ya-GST, YghU, zeta class glutathione S-transferase, Zeta class GST

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.18 glutathione transferase

Specific Activity

Specific Activity on EC 2.5.1.18 - glutathione transferase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000411
-
substrate butachlor
0.00065
-
crude enzyme of Wuzhou population
0.00087
-
substrate terbuthylazine
0.001
0.0012
-
crude enzyme of Nanyang population
0.002
-
purified recombinant mutant H167A, pH 6.5, 25°C, substrate 4-nitrobenzyl chloride
0.0021
-
crude enzyme of Hezhou population
0.0029 - 0.0036
-
with 1-chloro-2,4-dinitrobenzene as substrate
0.004
0.005
0.006
0.0075
-
using 1-chloro-2,4-dinitrobenzene as a substrate, at 25°C
0.0076
-
recombinant enzyme, substrate 1-chloro-2,4-dinitrobenzene
0.009
mutant enzyme F212Y, using 1,2-dichloro-4-nitrobenzene as a substrate
0.0091
with 1-chloro-2,4-dinitrobenzene as substrate, mutant enzyme Y12C, pH and temperature not specified in the publication
0.011
-
purified recombinant mutant F113A, pH 6.5, 25°C, substrate ethacrynic acid
0.012
mutant enzyme F212Y, using 4-nitrobenzyl chloride as a substrate
0.0147
-
using 1-chloro-2,4-dinitrobenzene as a substrate, at 25°C
0.0159
with 1-chloro-2,4-dinitrobenzene as substrate, wild type enzyme, pH and temperature not specified in the publication
0.017
0.018
mutant enzyme Q112A, using 4-nitrophenethyl bromide as a substrate
0.019
0.021
mutant enzyme F212W, using 4-nitrobenzyl chloride as a substrate
0.023
isozyme GST A3-3, using azathioprine as a substrate
0.024
-
with metolachlor as substrate
0.025
mutant enzyme Y215A, using 4-nitrobenzyl chloride as a substrate
0.026
mutant enzyme Y215S, using 1,2-dichloro-4-nitrobenzene as a substrate
0.029
0.0301
with hydrogen peroxide as substrate, mutant enzyme Y12C, pH and temperature not specified in the publication
0.034
mutant enzyme F212A, using 4-nitrobenzyl chloride as a substrate
0.035
0.036
0.038
Thermosynechococcus vestitus
using H2O2 as a substrate
0.039
using 3-iodopropylbenzene as substrate
0.041
0.043
0.044
mutant enzyme Q112A, using 1,2-dichloro-4-nitrobenzene as a substrate
0.04483
with 1-chloro-2,4-dinitrobenzene as substrate, at pH 7.5 and 25°C
0.048
mutant enzyme R214A, using 1,2-dichloro-4-nitrobenzene as a substrate
0.049
mutant enzyme Y215A, using 1,2-dichloro-4-nitrobenzene as a substrate
0.0494
-
purified enzyme of Nanyang population
0.052
mutant enzyme R214A, using 4-nitrobenzyl chloride as a substrate
0.053
mutant enzyme Y215H, using 4-nitrobenzyl chloride as a substrate
0.059
mutant enzyme F212L, using 4-nitrobenzyl chloride as a substrate
0.06
-
microsomal isozyme in presence of superoxide dismutase
0.0645
-
purified enzyme of Wuzhou population
0.066
using 3-iodopropylbenzene as substrate
0.067
with 4-hydroxynonenal as a substrate
0.0674
-
purified enzyme of Hezhou population
0.068
wild type enzyme, using 1,2-dichloro-4-nitrobenzene as a substrate
0.069
mutant enzyme Y215F, using 4-nitrophenethyl bromide as a substrate
0.073
mutant enzyme T174A, using ethacrynic acid as a substrate
0.075
-
microsomal isozyme in presence of Fe3+/ascorbate
0.079
wild type enzyme, using 4-nitrobenzyl chloride as a substrate
0.081
0.085
mutant enzyme F212A, using ethacrynic acid as a substrate
0.086
0.087
mutant enzyme F212L, using 1,2-dichloro-4-nitrobenzene as a substrate
0.088
-
purified recombinant mutant W164A, pH 6.5, 25°C, substrate ethacrynic acid
0.0907
with cumene hydroperoxide as substrate, mutant enzyme Y12C, pH and temperature not specified in the publication
0.0936
-
-
0.127 - 1.66
purified enzyme
0.139
-
microsomal isozyme
0.1396
with cumene hydroperoxide as substrate, wild type enzyme, pH and temperature not specified in the publication
0.14
Thermosynechococcus vestitus
using 1-chloro-2,4-dinitrobenzene as a substrate
0.141
-
in 0.1 M sodium phosphate buffer, pH 6.5, at 25°C
0.15
Thermosynechococcus vestitus
using ethacrynic acid as a substrate
0.165
-
after 162fold purification
0.19
-
with 1-chloro-2,4-dinitrobenzene as substrate, isoform GST7, at pH 7.0, temperature not specified in the publication
0.1991
with ethacrynic acid as substrate, mutant enzyme Y12C, pH and temperature not specified in the publication
0.203
GSTU17, substrate 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole
0.22
using 1,2-dichloro-4-nitrobenzene as a substrate
0.25
isozyme GSTS2, using ethacrynic acid as a substrate, at 25°C
0.29
mutant enzyme Y215F, using ethacrynic acid as a substrate
0.3
mutant enzyme Y215E, using ethacrynic acid as a substrate
0.3001
with ethacrynic acid as substrate, wild type enzyme, pH and temperature not specified in the publication
0.31
mutant enzyme F212Y, using ethacrynic acid as a substrate
0.312
-
with cumene hydroperoxide as substrate
0.335
purified recombinant enzyme, substrate 2-hydroxyethyl disulfide
0.36
-
substrate 1-chloro-2,4-dinitrobenzene, liver enzyme
0.38
using 1,6-diiodohexane as substrate
0.42
-
using 1,2-epoxy-3-(p-nitrophenoxy)propane as a substrate
0.48
-
purified recombinant mutant H167A, pH 6.5, 25°C, substrate 1-chloro-2,4-dinitrobenzene
0.49
-
with 1,2-epoxy-3-(4-nitrophenoxy)propane as substrate
0.57
wild type enzyme, using ethacrynic acid as a substrate
0.58
purified recombinant enzyme, substrate 1,2-dichloro-4-nitrobenzene
0.61
mutant enzyme Q112A, using ethacrynic acid as a substrate
0.63
-
with 1,2-epoxy-3-(4-nitrophenoxy)-propane as substrate, isoform GST20, at pH 7.0, temperature not specified in the publication
0.64
mutant enzyme Y215S, using ethacrynic acid as a substrate
0.67
-
purified recombinant mutant F113A, pH 6.5, 25°C, substrate 1-fluoro-2,4-dinitrobenzene
0.69
-
isoform GSTd14, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C
0.692
-
cytosolic isozyme in presence of Fe3+/ascorbate
0.702
-
cytosolic isozyme in presence of catalase
0.728
-
crude extract, at 25°C
0.76
-
Escherichia ccoli ATCC 25922
0.78
-
purified native enzyme
0.7975
with 4-nitrobenzyl chloride as substrate, mutant enzyme Y12C, pH and temperature not specified in the publication
0.84 - 0.92
-
with glutathione as substrate
0.86
-
with acrolein, isoenzyme A1-1
0.87
using 1,4-diiodobutane as substrate
0.9
with 1-chloro-2,4-dinitrobenzene as a substrate
0.91
using 1,4-diiodobutane as substrate
0.922
-
isoenzyme C
0.972
-
-
0.984
-
cytosolic isozyme in presence of superoxide dismutase
0.985
-
cytosolic isozyme
1.01
-
Escherichia coli ATCC 25422
1.06
isozyme GSTS2, using 1-chloro-2,4-dinitrobenzene as a substrate, at 25°C
1.0628
with 4-nitrobenzyl chloride as substrate, wild type enzyme, pH and temperature not specified in the publication
1.08
-
purified recombinant wild-type enzyme, pH 6.5, 25°C, substrate 1-chloro-2,4-dinitrobenzene
1.1
-
isoform GSTd10, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C
1.12
-
isoform GST1, with trans-trans-2,4-nonadienal as substrate, at pH 8.5 and 25°C
1.3
-
with ethacrynic acid as substrate, isoform GST7, at pH 7.0, temperature not specified in the publication
1.38
using iodohexane as substrate
1.49
-
isoform GST2, with trans-trans-2,4-decadienal as substrate, at pH 8.5 and 25°C
1.5 - 8.3
-
with cumene hydroperoxide as substrate
1.51
-
isoform GST2, with trans-trans-2,4-nonadienal as substrate, at pH 8.5 and 25°C
1.54
Thermosynechococcus vestitus
using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as a substrate
1.769
-
isoenzyme A
1.79
-
reaction with 1-chloro-2,4-dinitrobenzene
10.04
Thermosynechococcus vestitus
using trans-2-nonenal as a substrate
10.45
purified recombinant enzyme, substrate 1-chloro-2,4-dinitrobenzene in absence of 10 mM EDTA
10.5
-
crude extract, at pH 7.0 and 25°C
105
-
with 1-chloro-2,4-dinitrobenzene as substrate
105.45
wild type enzyme, at pH 7.5, temperature not specified in the publication
11
Thermosynechococcus vestitus
using cyclohexyl-isothiocyanate as a substrate
12.2
mutant enzyme F212A, using 1-chloro-2,4-dinitrobenzene as a substrate
12.3
mutant enzyme F212W, using 1-chloro-2,4-dinitrobenzene as a substrate
12.72
-
more anionic isoenzyme
12.8
using 1,2-diiodoethane as substrate
120.3
mutant enzyme F136A, at pH 7.5, temperature not specified in the publication
1220
-
after 265.9fold purification
124
-
-
129
-
purified recombinant isozyme GST P1-1
13.07
-
after 300fold purification, at 25°C
13.14
-
with 4-nitroquinoline-1-oxide as substrate, at pH 7.5 and 30°C
13.4
-
after 43.2fold purification, using 1-chloro-2,4-dinitrobenzene as a substrate
13.8
-
lung enzyme
14.56
purified recombinant enzyme, substrate 1-chloro-2,4-dinitrobenzene in presence of 10 mM EDTA
14.8
-
mutant enzyme R131W
15.47
-
less anionic isoenzyme
15.94
-
isoform GSTm09, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C
157
-
purified recombinant isozyme GST M1-1
16
-
isoenzyme beta
16.4
-
-
16.7 - 64.1
-
with 1-chloro-2,4-dinitrobenzene as substrate
17
-
isoenzyme gamma
1789.5
-
crude extract, at pH 6.0 and 40°C
182
-
purified recombinant isozyme GST M2-2
19.4
-
isoform GST2, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 8.5 and 25°C
2.3
-
with 1,2-dinitrobenzene
2.48
using cumene hydroperoxide as a substrate
2.52
mutant enzyme C14A/C47A/C101A/C169A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
2.57
isozyme GST A2-2, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate
2.66
-
crude extract, at pH 8.0 and 30°C
2.7
-
with 1,2-dichloro-4-nitrobenzene as substrate
20.39
-
purified native GSH-S transferase rho
20.4
-
anionic isozymes
21.03
isoform GSTmu, with 4-nitrophenethyl bromide as substrate, at pH 6.5 and 25°C
21.5
-
with 1-chloro-2,4-dinitrobenzene as substrate
22.69
-
purified recombinant mutant W164A, pH 6.5, 25°C, substrate 1-chloro-2,4-dinitrobenzene
22.9
mutant enzyme Y215A, using 1-chloro-2,4-dinitrobenzene as a substrate
25.2
mutant enzyme Y215A, using 1-chloro-2,4-dinitrobenzene as a substrate
25.6
-
-
254.9
-
-
26
-
using sulforaphane as a substrate
26.1
mutant enzyme Y215E, using 1-chloro-2,4-dinitrobenzene as a substrate
26.3
-
with acrolein, isoenzyme P1-1
26.6
using 1,2-diiodoethane as substrate
26.7
-
liver enzyme
26.9
-
isoform GST1, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 8.5 and 25°C
27
mutant enzyme Y215S, using 1-chloro-2,4-dinitrobenzene as a substrate
270
-
purified recombinant enzyme in presence of reduced glutathione
29.2
-
isoenzyme E
3
mutant enzyme I104V/A113V, with 7-chloro-4-nitrobenz-2-oxa-1,3-diazole
3 - 3.9
using 1,2-diiodoethane as substrate
3.03
mutant enzyme T174A, using 1-chloro-2,4-dinitrobenzene as a substrate
3.07
-
reaction with 1-chloro-2,4-dinitrobenzene
3.1
mutant enzyme C10A, at 30°C
3.3
after 9.7fold purification, in 0.1 M potassium phosphate buffer (pH 6.5), at 25°C
3.31
-
purified recombinant mutant S110a, pH 6.5, 25°C, substrate 1-fluoro-2,4-dinitrobenzene
3.34
-
isoform GSTm09, with cumene hydroperoxide as substrate, at pH 6.5 and 25°C
3.4
mutant enzyme R70Q, in phosphate buffer (pH 6.5), at 25°C
3.51
using ethacrynic acid as a substrate
3.6 - 11.8
-
with cumene hydroperoxide as substrate
3.69
-
purified recombinant wild-type enzyme, pH 6.5, 25°C, substrate 1-fluoro-2,4-dinitrobenzene
3.83
purified protein with poly(His) tag, with acrolein as substrate, at pH 6.0 and 25°C
300.6
purified native enzyme
31.2
purified liver GST, substrate 1-chloro-2,4-dinitrobenzene
31.5
using 1,2-diiodoethane as substrate
32.2
mutant enzyme F212L, using 1-chloro-2,4-dinitrobenzene as a substrate
325
-
using 1-chloro-2,4-dinitrobenzene as a substrate
33
Thermosynechococcus vestitus
using phenethyl-isothiocyanate as a substrate
33 - 94
-
with 1-chloro-2,4-dinitrobenzene as substrate
34
-
isoenzyme epsilon
34.55
-
after 162fold purification
34.7
purified recombinant enzyme, substrate 1-chloro-2,4-dinitrobenzene
36
with allyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication
36.13
mutant enzyme C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
36.2
mutant enzyme Y215H, using 1-chloro-2,4-dinitrobenzene as a substrate
37
-
isoenzyme delta
37.1
-
mutant enzyme R131M
38.2
-
with 1,2-dichloro-4-nitrobenzene as substrate
3980.6
-
after 2.22fold purification, at pH 6.0 and 40°C
4
mutant enzyme A113V, with 7-chloro-4-nitrobenz-2-oxa-1,3-diazole
4.04
-
with hexa-2,4-dienal as substrate, at pH 7.5 and 30°C
4.1
-
isoenzyme A
4.18
-
Klebsiella oxytoca CIP 666
4.28
mutant enzyme F212H, using 1-chloro-2,4-dinitrobenzene as a substrate
4.33
purified overall GST activity in adult, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication
4.41
isoform GSTmu, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C
4.52
using 1,2-diiodoethane as substrate
4.54
-
with trans-4-phenyl-3-butene-2-one as substrate, at pH 7.5 and 30°C
4.57
purified overall GST activity in eggs, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication
4.58
-
isozyme GST A1-1, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate
4.6
-
somatic extract
4.75
mutant enzyme C14A/C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
4.8
-
Klebsiella oxytoca AF101
4.9
-
with 1,2-dichloro-4-nitrobenzene as substrate
4.96
-
purified wild-type GST T1-1, substrate 1-iodomethane
40
-
using trans-4-phenyl-3-butene-2-one as a substrate
42.8
-
after 16.1fold purification, at pH 8.0 and 30°C
44.3
mutant enzyme R214A, using 1-chloro-2,4-dinitrobenzene as a substrate
440.1
-
purified native enzyme
4610
-
after 6332fold purification, at 25°C
5
Thermosynechococcus vestitus
using sulforaphane as a substrate
5.1
-
with trans-2-nonenal as substrate, at pH 7.5 and 30°C
5.2
-
with 0.1 mM 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.0 and 25°C
5.26
-
using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as a substrate
5.35
isoform GSTmu, with 4-nitrobenzyl chloride as substrate, at pH 6.5 and 25°C
5.6
wild type enzyme, using 1-chloro-2,4-dinitrobenzene as a substrate
5.9
wild type enzyme, at 30°C
51.5
using 1-chloro-2,4-dinitrobenzene as a substrate
52.4
mutant enzyme Q112A, using 1-chloro-2,4-dinitrobenzene as a substrate
53.2
isozyme GST A2-2, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate
56.4
using 1,2-diiodoethane as substrate
56.5 - 79.4
-
-
58.6
wild type enzyme, using 1-chloro-2,4-dinitrobenzene as a substrate
6.082
recombinant enzyme, at 25°C
6.17
purified overall GST activity in larvae, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication
6.6
-
activity with 2,3-hexadienal
6.79
isoform GSTrho, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C
60
mutant enzyme I104V, with 1-chloro-2,4-dinitrobenzene
60.2
-
after 5.73fold purification, at pH 7.0 and 25°C
61.07
His-tagged wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
61.5
-
wild-type enzyme
61.8
isozyme GST A1-1, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate
68.5
-
purified GST, substrate 1-chloro-2,4-dinitrobenzene
68.54
-
with 1-chloro-2,4-dinitrobenzene as substrate, at pH 7.5 and 30°C
7.05
-
with acrolein, isoenzyme M1-1
7.1
-
activity with 1,2-dichloro-4-nitrobenzene
7.26
isozyme GST A3-3, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate
7.84
-
purified wild-type GST T1-1, substrate 1,2-epoxy-3-(4-nitro-phenoxy)propane
7.89
-
purified recombinant mutant W164A, pH 6.5, 25°C, substrate 1-fluoro-2,4-dinitrobenzene
7.9 - 41.1
-
with 1-chloro-2,4-dinitrobenzene as substrate
70
mutant enzyme I104V/A113V, with 1-chloro-2,4-dinitrobenzene
71.7
untagged wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
74
with phenethyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication
74.3
mutant enzyme Y215F, using 1-chloro-2,4-dinitrobenzene as a substrate
77
-
with adenine propenal, isoenzyme P1-1
77.7
-
purified GST, substrate ethacrynic acid
8.03
mutant enzyme F212Y, using 1-chloro-2,4-dinitrobenzene as a substrate
80 - 272
-
with 1-chloro-2,4-dinitrobenzene as substrate
82.27
-
GST I-I
84
-
purified recombinant isozyme GST A1-1
93.21
-
GST II-III
additional information