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2.5.1.17: corrinoid adenosyltransferase

This is an abbreviated version!
For detailed information about corrinoid adenosyltransferase, go to the full flat file.

Word Map on EC 2.5.1.17

Reaction

2 ATP + 2 cob(II)alamin +

a reduced flavoprotein
= 2 triphosphate + 2 adenosylcob(III)alamin +
an oxidized flavoprotein

Synonyms

ACAT, adenosyltransferase, vitamin B12s, aquacob(I)alamin adenosyltransferase, aquocob(I)alamin adenosyltransferase, ATP: cobalamin adenosyltransferase, ATP:co(I)rrinoid adenosyltransferase, ATP:cob(I)alamin adenosyltransferase, ATP:cob(I)alamin Cobeta-adenosyltransferase, ATP:cob(I)alamin transferase (ATR), ATP:Cob(I)alaminadenosyltransferase, ATP:cobalamin adenosyltransferase, ATP:cobalt(I) corrinoid adenosyltransferase, ATP:corrinoid adenosyltransferase, ATP:corrinoid adenosyltransferase PduO, ATR, cob(I)alamin adenosyltransferase, cob(I)yrinic acid a,c-diamide adenosyltransferase, CobA, CobA-type ATP:Co(I)rrinoid adenosyltransferase, cobalamin adenosyltransferase, CobAMm, cobO mutant SVQ336, cobO mutant SVQ524, cobO mutantSVQ336, EC 1.16.8.1, EutT, EutT adenosyltransferase, hATR, homologeous to PduO-type ATP: cob(I)alamin adenosyltransferase, human adenosyltransferase, human ATP: cob(I)alamin adenosyltransferase, human-type ACA, human-type ATP: co(I)rrinoid adenosyltransferase, human-type ATP: cob(I)alamin adenosyltransferase, LrPduO, methylmalonic aciduria type B, MMAB, MMAB protein, PduO, PduO adenosyltransferase, PduO enzyme, PduO protein, PduO-type ACA, PduO-type adenosine 5'-triphosphate:corrinoid adenosyltransferase, PduO-type ATP: cobalamin adenosyltransferase, PduO-type ATP:Co(I)rrinoid adenosyltransferase, PduO-type ATP:cob(I)alamin adenosyltransferase, PduO-type ATP:corrinoid adenosyltransferase, PduO-type corrinoid adenosyltransferase, ST1454, ST2180, TA1434, vitamin B12s adenosyltransferase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.17 corrinoid adenosyltransferase

Cloned

Cloned on EC 2.5.1.17 - corrinoid adenosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview
DNA sequence determination and analysis, expression of 2 polymorphic variants 239K and 239M of the enzyme in Escherichia coli as soluble proteins
-
expressed in Escherichia coli
expressed in Escherichia coli as an N-terminal His-tagged fusion protein
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli as a 6His-tagged fusion protein
expression in Escherichia coli as a His-tagged protein
expression in Escherichia coli in strain BL21(DE3) of the wild-type enzyme and in strain B834(DE3) as selenomethionine-labeled enzyme
expression of the enzyme in C57/Bl6 mice using an adeno-associated virus vector, rAAV 2 and 8 vectors, with primer pairs specific to the cytomegalovirus enhancer/chicken beta-actin, CBAT, promoter, quantitative and semiquantitative expression analysis in murine liver, overview
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expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha as maltose-binding-protein or GST fusion protein
-
expression of wild-type enzyme in Escherichia coli in inclusion bodies, expression of truncated mutants in Escherichia coli
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from genomic DNA in pET-28a for expression in Escherichia coli BL21 (DE3)
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from genomic DNA in pET20b for expression with N-terminal hexa-His tag in Escherichia coli strain BL21 (DE3)
from genomic DNA in pET20b for expression with N-terminal hexa-His tag in Escherichia coli strain BL21 (DE3) or B834 (DE3) for the selenomethionine derivative
from genomic DNA in pET28a for expression with a C-terminal hexa-His tag in Escherichia coli BL21 (DE3)
gene cobA, recombinant overexpression of tagless, wild-type CobA and expression of His7-tagged mutant CobAs in Escherichia coli strain BL21(lambdaDE3) carrying a null allele of btuR, the cobA homologue in this bacterium
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gene cobAMm, expression in a Salmonella enterica strain lacking the housekeeping CobA enzyme restores coenzyme B12 synthesis, overexpression in Escherichia coli strain BL21(DE3)
-
gene eutT, overexpression of His-tagged enzyme in Escherichia coli as insoluble protein
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gene pduO, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene pduO, overexpression in Escherichia coli strain BL21 (DE3) and secretion to the medium
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gene pduO, recombinant overexpression of His-tagged wild-type and mutant PduOs in Escherichia coli
-
overexpression of wild-type and mutant enzymes in Escherichia coli using strain BL21(DE3) for the His-tagged variant, and several derivatives of strain DH5alpha, overview
-
QuickChangeXL site-directed mutagenesis performed on wild-type LrpduO in pTEV3 before expression of wild-type and variants in Escherichia coli BL21(DE3) with a rTEV protease-cleavable N-terminal hexa-His tag
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recombinantly expressed in Escherichia coli
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structural gene cobA
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without mitochondrial targeting sequence from plasmid pNL166 harbouring the coding sequence, native or with C-terminal octa-His tag into pTA925 to introduce a linker region necessary for expression in Escherichia coli BL21DE3 RIL and ATR-deficient Salmonella strain BE620, hATR in pCF13 (N-terminal octa-His tag) and pCF15 (C-terminal octa-His tag) for transformation and random mutagenesis in Escherichia coli mutator strain XL1-Red followed by expression in BE620