2.5.1.16: spermidine synthase

This is an abbreviated version!
For detailed information about spermidine synthase, go to the full flat file.

Word Map on EC 2.5.1.16

2.5.1.16

polyamine

spermine

s-adenosylmethionine

ornithine

diamine

samdc

dicyclohexylamine

alpha-difluoromethylornithine

5'-methylthioadenosine

cadaverine

trypanothione

adometdc

agmatine

4.1.1.50

difluoromethylornithine

medicine

drug development

methylthioadenosine

thermospermine

nutrition

1,3-diaminopropane

biotechnology

norspermidine

multisubstrate

Reaction

S-adenosyl 3-(methylsulfanyl)propylamine

Synonyms

aminopropyltransferase, aminopropyltransferase spermidine synthase, AtSPDS1, AtSPDS2, MdSPDS1, PAPT, PgSPD, putrescine aminopropyltransferase, Spd synthase, SPDS, SPDS2, SPDSYN, spe-sdh, spe3, SpeE, spermidine synthase, spermidine synthase 1, spermidine synthetase, Spm synthase, SpSyn, Synpcc7942_0628, synthase, spermidine

ECTree

2 Transferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

2.5.1.16 spermidine synthase

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Results	in table
2	Activating Compound
21026	AA Sequence
5	Application
1	CAS Registry Number
40	Cloned(Commentary)
4	Cofactor
14	Crystallization (Commentary)
93	Disease/ Diagnostics
2	Expression
30	General Information
14	General Stability
10	IC50 Value
198	Inhibitors
6	kcat/KM [mM/s]
10	Ki Value [mM]
51	KM Value [mM]
6	Localization
13	Metals/Ions
53	Molecular Weight [Da]
73	Natural Substrates/ Products (Substrates)
650	Organism
7	Pathway
196	PDB ID
27	pH Optimum
5	pH Range
4	pH Stability
2	pI Value
24	Protein Variants
42	Purification (Commentary)
15	Reaction
1	Reaction Type
125	Reference
118	Source Tissue
18	Specific Activity [micromol/min/mg]
18	Storage Stability
206	Substrates and Products (Substrate)
41	Subunits
80	Synonyms
1	Systematic Name
37	Temperature Optimum [°C]
4	Temperature Range [°C]
8	Temperature Stability [°C]
22	Turnover Number [1/s]

Purification

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Purification on EC 2.5.1.16 - spermidine synthase

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7 entries

2000fold purified

Escherichia coli

639706

affinity chromatography

Bos taurus

489868

affinity chromatography on S-adenosyl(5')-3-thiopropylamine-Sepharose

Rattus norvegicus

639709

affinity chromatography with S-adenosyl-5'-3-thiopropylamine linked to Sepharose, 11000fold purified

Bos taurus

639697

ammonium sulfate fractionation, acetone precipitation and chromatography on Sephadex G-100, 160fold purified

Brassica rapa subsp. pekinensis

489871

ammonium sulfate fractionation, affinity chromatography and gel filtration, 1900fold purified

Brassica rapa subsp. pekinensis

4666

ammonium sulfate fractionation, chromatography on DEAE-cellulose, affinity chromatography, 265fold purified

Rattus norvegicus

639701

ammonium sulfate fractionation, chromatography on DEAE-Cellulose, affinity chromatography, 8700fold purified

Homo sapiens

489868

ammonium sulfate fractionation, chromatography on DEAE-Sephacel, chromatography on Sephacryl S-300, chromatography on aminooctyl-Sepharose and ATPA-Sepharose, 319fold purified

Glycine max

639714

chromatography on Sephadex G-25 and DEAE-cellulose chromatography

Rattus norvegicus

639700

extraction, ammonium sulfate fractionation, chromatography on calcium phosphate cellulose, DEAE-Sephadex, chromatography on hydroxyapatite, disc gel electrophoresis

Escherichia coli

639707

extraction, ammonium sulfate fractionation, chromatography on DEAE-cellulose, affinity chromatography, gel filtration, 4480fold purified

Rattus norvegicus

639705

extraction, ammonium sulfate fractionation, chromatography on DEAE-Cellulose, chromatography on hydroxyapatite, chromatography on ATPA-Sepharose, gel filtration

Bos taurus

639705

immobilized metal affinity column chromatography

Leishmania donovani

A4HS53

737602

metal affinity chromatography

3 entries

metal affinity chromatography and gel filtration

Plasmodium falciparum

Q9NFS5

662805

Ni-affinity column chromatography

Plasmodium falciparum

Q8II73

737358

Ni-NTA affinity chromatography

Helicobacter pylori

O25503

663317

Ni-NTA column chromatography and Superdex 75 gel filtration

partial

partial, streptomycin sulfate precipitation, ammonium sulfate fractionation and gel filtration, 18fold purified

recombinant enzyme

recombinant enzyme from Escherichia coli by affinity chromatography

Solanum tuberosum

Q93X16

723374

recombinant FLAG-tagged enzyme from HEK-293 cells by affinity chromatography

Homo sapiens

671298

recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography

Homo sapiens

P19623

672272

recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography

Mus musculus

675523

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain SG13009

Escherichia coli

P09158

723164

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography

Helicobacter pylori

677059

streptomycin and ammonium sulfate fractionation, acetone precipitation and chromatography on Sephadex G-100, 160fold purified

Brassica rapa subsp. pekinensis

639704

using Ni-NTA chromatography

ventral prostate