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2.5.1.101: N,N'-diacetyllegionaminate synthase

This is an abbreviated version!
For detailed information about N,N'-diacetyllegionaminate synthase, go to the full flat file.

Word Map on EC 2.5.1.101

Reaction

2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose
+
phosphoenolpyruvate
 +
H2O
=
N,N'-diacetyllegionaminate
 +
phosphate

Synonyms

legI, N,N'-diacetyllegionaminic acid synthase, NeuB, NeuB homologue, nonulosonic acid synthase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.101 N,N'-diacetyllegionaminate synthase

Cloned

Cloned on EC 2.5.1.101 - N,N'-diacetyllegionaminate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene legI or Cj1327, DNA and ammino acid sequence determination and analysis, expression as N-terminally His6-tagged enzyme
-
gene lpg 0752, cloning and expression of NeuB results in insoluble protein which possesses very low levels of N,N'-diacetyllegionaminate synthase activity
-
gene lpg0752 or neuB, DNA and amino acid sequence determination and analysis, overexpression of the inactive C- and N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), functional expression as MalE-NeuB fusion protein
-