2.4.99.B5: acarviosyl transferase

This is an abbreviated version!
For detailed information about acarviosyl transferase, go to the full flat file.

Reaction

Synonyms

AcbD, ATASE

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.99 Transferring other glycosyl groups

                2.4.99.B5 acarviosyl transferase

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Results	in table
1	AA Sequence
1	Cloned(Commentary)
1	Inhibitors
2	KM Value [mM]
2	Localization
1	Metals/Ions
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
3	Organism
2	pH Optimum
1	pI Value
9	Protein Variants
1	Purification (Commentary)
1	Reaction
3	Reference
1	Specific Activity [micromol/min/mg]
21	Substrates and Products (Substrate)
2	Subunits
3	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]

Engineering

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Engineering on EC 2.4.99.B5 - acarviosyl transferase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D229N

Actinoplanes sp.

-

mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity

729196

D328N

Actinoplanes sp.

-

mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity

729196

E257Q

Actinoplanes sp.

-

mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity

729196

G140H

2 entries

G140H/Q327H

Actinoplanes sp.

-

mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 1.2% of starch into cyclodextrins

729196

G140H/Q327H/T329M

Actinoplanes sp.

-

mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 7.4% of starch into cyclodextrins

729196

Q327H

Actinoplanes sp.

-

mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is reduced

729196

T329M

Actinoplanes sp.

-

mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is strongly enhanced

729196

G140H

Actinoplanes sp.

-

mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 2% of starch into cyclodextrins

729196

G140H

Actinoplanes sp.

-

mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is strongly enhanced

729196