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2.4.99.21: dolichyl-phosphooligosaccharide-protein glycotransferase

This is an abbreviated version!
For detailed information about dolichyl-phosphooligosaccharide-protein glycotransferase, go to the full flat file.

Word Map on EC 2.4.99.21

Reaction

an archaeal dolichyl phosphooligosaccharide
+
[protein]-L-asparagine
=
an archaeal dolichyl phosphate
+
a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine

Synonyms

AF_0380, AglB, AglB protein, AglB-L, aglB1, AlgB, archaeal oligosaccharyl transferase, dolichyl-monophosphooligosaccharide-protein glycotransferase, Mv1749, MVO1749

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.99 Transferring other glycosyl groups
                2.4.99.21 dolichyl-phosphooligosaccharide-protein glycotransferase

Systematic Name

Systematic Name on EC 2.4.99.21 - dolichyl-phosphooligosaccharide-protein glycotransferase

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SYSTEMATIC NAME
IUBMB Comments
dolichyl-phosphooligosaccharide:protein-L-asparagine N-beta-D-oligosaccharidotransferase
The enzyme, characterized from the archaea Methanococcus voltae and Haloferax volcanii, transfers a glycan component from dolichyl phosphooligosaccharide to external proteins. It is different from EC 2.4.99.18, dolichyl-diphosphooligosaccharide-protein glycotransferase, which uses dolichyl diphosphate as carrier compound in bacteria and eukaryotes. The enzyme participates in the N-glycosylation of proteins in some archaea. It requires Mn2+. Dolichol used by archaea is different from that used by eukaryotes. It is much shorter (C55-C60), it is alpha,omega-saturated and it may have additional unsaturated positions in the chain.