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2.4.99.14: (KDO)2-lipid IVA (2-8) 3-deoxy-D-manno-octulosonic acid transferase

This is an abbreviated version!
For detailed information about (KDO)2-lipid IVA (2-8) 3-deoxy-D-manno-octulosonic acid transferase, go to the full flat file.

Reaction

alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
+
CMP-beta-Kdo
=
alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
+
CMP

Synonyms

3-deoxy-D-manno-oct-2-ulosonic acid transferases, Kdo transferase, multi-functional Kdo-transferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.99 Transferring other glycosyl groups
                2.4.99.14 (KDO)2-lipid IVA (2-8) 3-deoxy-D-manno-octulosonic acid transferase

Cloned

Cloned on EC 2.4.99.14 - (KDO)2-lipid IVA (2-8) 3-deoxy-D-manno-octulosonic acid transferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in a rough mutant (Re chemotype) of Escherichia coli (strain F515) that contains an lipopolysaccharide with only two alpha 2-4-linked Kdo residues. Recombinant strains are able to add the immunodominant Kdo residue in alpha 2-8-linkage to the parental lipopolysaccharide. Comparison of nucleotide and the deduced amino acid sequences of gseA of Chlamydia psittaci 6BC and Chlamydia trachomatis L
expressed in Corynebacterium glutamicum
expression in Escherichia coli
expression in Escherichia coli. Chlamydial Kdo transferases can replace in Escherichia coli K-12 the host's Kdo transferase and retain the product specificities described in their natural background. WaaA from Chlamydia psittaci transfers predominantly four Kdo residues to lipid A, forming a branched tetrasaccharide with the structure alpha-Kdo-(2,8)-[alpha-Kdo-(2,4)]-alpha-Kdo-(2,4)-alpha-Kdo
-
introduction of gseA into an Escherichia coli mutant with a thermolabile kdtA gene product endows cell extracts with the ability to transfer not only the third but also the first two Kdos to lipid A precursors, the Chlamydia trachomatis enzyme is at least trifunctional
-