Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.2.7: adenine phosphoribosyltransferase

This is an abbreviated version!
For detailed information about adenine phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.7

Reaction

AMP
+
diphosphate
=
adenine
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

adenine phosphoribosyl transferase, adenine phosphoribosylpyrophosphate transferase, adenine phosphoribosyltransferase, adenine phosphoribosyltransferase 2, adenione PRTase, adenosine phosphoribosyltransferase, adenylate pyrophosphorylase, adenylic pyrophosphorylase, AMP pyrophosphorylase, AMP-pyrophosphate phosphoribosyltransferase, AMP:pyrophosphate phospho-D-ribosyltransferase, APRT, APRT1, APRT2, APRTase, APRTh, apT, APT1, APT2, APT4, APT5, ApTR, FtAPRT, gpT-1, hAPRT, More, phosphoribosyltransferase, adenine, ScAPRT, SmAPRT1, Smp_054360, SSO2342, transphosphoribosidase, TtAPRT2, TTC1249, TTC1250, TTH1250, TTHA1614, TthAPRT, TthHB27APRT, TT_C1249, TT_C1250, type I PRT

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.7 adenine phosphoribosyltransferase

Purification

Purification on EC 2.4.2.7 - adenine phosphoribosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 isoforms recombinant from Escherichia coli as His-tagged proteins
copurification of N-terminally His-tagged ARTh and GDH from Escherichia coli
hydroxyapathite column chromatography
native enzyme by heat treatment for 10 min at 65°C, desalting gel filtration, anion exchange and hydrophobic interaction chromatography, ultrafiltration, and gel filtration
Ni affinity column chromatography
recombinant APRT from Escherichia coli strain BL21(DE3)/pER by hydrophobic interaction chromatography and ultrafiltration
recombinant enzyme from Escherichia coli strain BL21(DE3)
recombinant from Escherichia coli
-
recombinant His-tagged enzyme from Escherichia coli
recombinant His-tagged isozyme SmAPRT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, tag cleavage by thrombin, and again gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through thrombin, followed by dialysis
recombinant His-tagged wild-type and mutants from Escherichia coli
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by heat treatement, nickel affinity chromatography, and gel filtration
recombinant His6-tagged TtAPRT from Escherichia coli strain BL21(DE3) by nickel affinity chromatograph, tag cleavage by thrombin, and gel filtration. Recombinant nontagged enzyme from Escherichia coli strain BL21 Codon Plus (DE3)-RIL by heat treatment at 70°C for 15 min in a digital sonifier, two different steps of anion exchange chromatography, intervening desalting gel filtration, and hydroxyapatite chromatography, followed by ultrafiltration, and gel filtration
recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) and recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834 by two steps of anion exchange chromatography, and gel filtration
-