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dimer
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2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 20440, His-tagged recombinant enzyme, SDS-PAGE
?
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x * 17000, SDS-PAGE
?
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x * 27000, recombinant soluble enzyme, SDS-PAGE
?
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x * 27000, recombinant soluble enzyme, SDS-PAGE
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?
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x * 18000, SDS-PAGE, x * 18280, calculated
?
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x * 18000, SDS-PAGE, x * 18280, calculated
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hexamer
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6 * 18000, DRTase II, SDS-PAGE
hexamer
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6 * 20000, DRTAse I
hexamer
6 * 18000, SDS-PAGE
homodimer
2 * 19000, recombinant enzyme, SDS-PAGE
homodimer
2 * 19000, recombinant enzyme, SDS-PAGE, 2 * 19090, sequence calculation
homodimer
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2 * 19000, recombinant enzyme, SDS-PAGE
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homodimer
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2 * 19000, recombinant enzyme, SDS-PAGE, 2 * 19090, sequence calculation
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homohexamer
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6 * 16000, SDS-PAGE
homohexamer
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6 * 16000, SDS-PAGE
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homotetramer
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homotetramer
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4 * 17250, elution as single peak from the preparative gel filtration column
monomer
1 * 21000, recombinant His-tagged enzyme, SDS-PAGE and gel filtration
monomer
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1 * 21000, recombinant His-tagged enzyme, SDS-PAGE and gel filtration
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monomer
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1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE
additional information
LhNDT is a monomer as revealed by gel filtration with or without addition of Ca2+ in elution buffer
additional information
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LhNDT is a monomer as revealed by gel filtration with or without addition of Ca2+ in elution buffer
additional information
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LhNDT is a monomer as revealed by gel filtration with or without addition of Ca2+ in elution buffer
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additional information
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at 0.003 mM the His-RCL is mostly monomeric, at 0.03 mM, His-RCL is mostly dimeric, at 0.01 mM His-RCL is at equilibrium between monomeric and dimeric states, overview
additional information
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ligand binding and active site structures, overview
additional information
in general, a higher oligomerization state for NDTs is not essential for catalysis and/or stabilization of the active conformation. Binding energy analysis of the TbPDT dimer interface, overview
additional information
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in general, a higher oligomerization state for NDTs is not essential for catalysis and/or stabilization of the active conformation. Binding energy analysis of the TbPDT dimer interface, overview
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