preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol
recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli to homogeneity by nickel affinity chromatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, preparative gel fitration, dialysis, and ultrafiltration
recombinant unmodified enzyme from Escherichia coli strain Bli5 (BL21 (DE3) pDIA17) by anion exchange chromatography and gel filtration, recombinant His-tagged enzyme by nickel affinity chromatography, over 95% purity