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2.4.2.6: nucleoside deoxyribosyltransferase

This is an abbreviated version!
For detailed information about nucleoside deoxyribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.6

Reaction

2-deoxy-D-ribosyl-base1
+
base2
=
2-deoxy-D-ribosyl-base2
+
base1

Synonyms

2'-deoxyribosyltransferase, 2'-N-deoxyribosyltransferase, Chro_1188, CtNDT, deoxynucleoside 5-monophosphate N-glycosidase, deoxyribose transferase, deoxyribosyltransferase, nucleoside, DRTase I, DRTase II, GQR93_14380, LaNDT, LhNDT, LlNDT, LmPDT, LrNDT, N-deoxyribosyltransferase, N-deoxyribosyltransferase II, NDRT, NdRT-II, NDT, NDT-II, NDT4, NTD, nucleoside 2'-deoxyribosyltransferase, nucleoside 2'-deoxyribosyltransferase type II, nucleoside 2-deoxyribosyltransferase, nucleoside deoxyribosyltransferase I, nucleoside deoxyribosyltransferase II, nucleoside N-deoxyribosyltransferase, nucleoside trans-N-deoxyribosylase, PDT, purine nucleoside 2'-deoxyribosyltransferase, purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyl transferase, RCL, Tb927.5.1360, TbPDT, trans-deoxyribosylase, trans-N-deoxyribosylase, trans-N-glycosidase, transdeoxyribosylase, type I NDT, YejD

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.6 nucleoside deoxyribosyltransferase

Purification

Purification on EC 2.4.2.6 - nucleoside deoxyribosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Sephacryl S-200 gel filtration
-
Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration
-
DRTase I and DRTAse II
-
preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel fitration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and tag cleavage through thrombin
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, method optimization for LaNDT
-
recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli to homogeneity by nickel affinity chromatography and gel filtration
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, preparative gel fitration, dialysis, and ultrafiltration
recombinant unmodified enzyme from Escherichia coli strain Bli5 (BL21 (DE3) pDIA17) by anion exchange chromatography and gel filtration, recombinant His-tagged enzyme by nickel affinity chromatography, over 95% purity
-