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2.4.2.43: lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase

This is an abbreviated version!
For detailed information about lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.43

Reaction

4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate
+
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A
=
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A
 +
ditrans,octacis-undecaprenyl phosphate

Synonyms

4-amino-4-deoxy-L-arabinose transferase, aminoarabinose transferase, arnT, L-Ara4N transferase, undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.43 lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase

Engineering

Engineering on EC 2.4.2.43 - lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E493C
-
inactive
K69C
-
inactive
R254C
-
inactive
Y34C
-
inactive
C148A/C149A/C173S/C216S/C318S/C383S/C400S/C411S
-
when assayed for growth in the presence of polymyxin, the cysteine-free construct of ArnT supports growth at a level similar to that of the native protein
C148S/C149S/C173S/C216S/C318S/C383S/C400S/C411S
-
mutant enzyme is inactive
C173S/C216S/C318S/C383S/C400S/C411S
-
when assayed for growth in the presence of polymyxin, the cysteine-free construct of ArnT supports growth at 70% of the native protein
additional information
-
analysis of 31 point mutations within a putative periplasmic loop of the cysteine-free ArnT protein, carried out using an in vivo growth assay coupled with expression studies, identification of the first time specific critical residues within the bacterial transferase. These critical residues fall into two categories: those that disrupt initial protein folding or membrane localization and those that fail to confer in vivo resistance to polymyxin despite being expressed to the inner membrane