2.4.2.3: uridine phosphorylase

This is an abbreviated version!
For detailed information about uridine phosphorylase, go to the full flat file.

Reaction

Synonyms

apUP, EC 2.4.2.23, L-UrdPase, More, PcUP1, PcUP2, phosphorylase, uridine, pynpase, pyrimidine nucleoside phosphorylase, pyrimidine phosphorylase, pyrimidine/purine nucleoside phosphorylase, StUPh, udp, UDRPase , UP type 1, UP1, UP2, uPA, UPase, UPase-2, UPb, UPH, UPP1, UPP2, UrdPase, uridine phosphorylase, uridine phosphorylase 1, uridine phosphorylase-1, uridine phosphorylase-2, uridine:orthophosphate alpha-D-ribosyltransferase, VchUPh

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.2 Pentosyltransferases

                2.4.2.3 uridine phosphorylase

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Results	in table
5	Activating Compound
5766	AA Sequence
9	Application
1	CAS Registry Number
32	Cloned(Commentary)
31	Crystallization (Commentary)
354	Disease/ Diagnostics
7	Expression
19	General Information
4	General Stability
127	Inhibitors
8	kcat/KM [mM/s]
39	Ki Value [mM]
85	KM Value [mM]
10	Localization
2	Metals/Ions
29	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
667	Organism
8	Pathway
483	PDB ID
23	pH Optimum
7	pH Range
1	pH Stability
78	Protein Variants
46	Purification (Commentary)
18	Reaction
3	Reaction Type
127	Reference
1	Renatured (Commentary)
86	Source Tissue
20	Specific Activity [micromol/min/mg]
6	Storage Stability
200	Substrates and Products (Substrate)
35	Subunits
81	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
1	Temperature Range [°C]
8	Temperature Stability [°C]
6	Turnover Number [1/s]

Application

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Application on EC 2.4.2.3 - uridine phosphorylase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

diagnostics

2 entries

drug development

Homo sapiens

Q16831

UP12 is a target for development of enzyme inhibitors for cancer chemotherapy, design of inhibitors is intended to boost endogenous uridine levels to rescue normal tissues from the toxicity of fluoropyrimidine nucleoside chemotherapeutic agents, such as capecitabine and 5-fluorouracil.

701939

medicine

2 entries

synthesis

4 entries

diagnostics

Homo sapiens

Q16831

UPP1 may serve as a negative prognosticator in glioma, analysis of the prognostic value of UPP1 for glioma, overview. UPP1 predicts shorter survival for glioma

758991

diagnostics

Homo sapiens

Q16831

upregulation of UPP1 increases lymph node metastasis risk and is useful as diagnostic, prognostic, and predictive biomarker in thyroid cancer

759543

medicine

Mus musculus

-

uridine phosphorylase plays an important role in the antineoplastic activity of 5-fluorouracil and in the anabolism of its oral prodrug, capecitabine, through the conversion of 5'-deoxy-5-fluorouridineinto 5-fluorouracil. TNF-alpha efficiently induces UPase gene expression through a NF-kappaB subunit p65-dependent pathway enhancing cell sensitivity to 5'-deoxy-5-fluorouridine. The elucidation of this regulation mechanism may aid in the clinical use of 5-fluorouracil-based chemotherapy

676094

medicine

Homo sapiens

-

the paired expression level of the uridine phosphorylase gene in gastric cancer is a possible prognostic marker for patients with 5-fluorouracil treatment

684470

synthesis

Escherichia coli

-

evolvement of a mutant enzyme by iterative saturation mutagenesis. Compared to the wild type enzyme, which has a temperature optimum of 40°C and a half-life of 9.89 h at 60°C, the selected mutant has a temperature optimum of 60°C and a half-life of 17.3 h at 60°C. Self-immobilization of the native enzyme as a Spherezyme shows a 3.3fold increase in thermostability while immobilized mutant enzyme shows a 4.4fold increase in thermostability. Combining the enzyme with the purine nucleoside phosphorylase from Bacillus halodurans allows for synthesis of 5-methyluridine (a pharmaceutical intermediate) from guanosine and thymine in a one-pot transglycosylation reaction. Replacing the wild type uridine phosphorylase with the mutant allows for an increase in reaction temperature to 65°C and increased the reaction productivity from 10 to 31 g per l and h

723000

synthesis

Aeropyrum pernix

Q9YA34

two-step efficient synthesis of 5-methyluridine, a intermediate in the synthesis of anti-AIDS drug such as stavudine and zidovudine

721810

synthesis

Aeropyrum pernix

Q9YA34

the enzyme is a valuable industrial biocatalysts for high-temperature reactions that produce nucleoside drugs in high yields

726781

synthesis

Aeropyrum pernix DSM 11879

-

the enzyme is a valuable industrial biocatalysts for high-temperature reactions that produce nucleoside drugs in high yields

-