2.4.2.29: tRNA-guanosine34 preQ1 transglycosylase

This is an abbreviated version!
For detailed information about tRNA-guanosine34 preQ1 transglycosylase, go to the full flat file.

Reaction

Synonyms

ArcTGT, guanine insertion enzyme, guanine, queuine-tRNA transglycosylase, Q-insertase, QTRT1, QueTGT, queuine insertase, queuine tRNA ribosyltransferase, queuine tRNA-ribosyltransferase, queuine tRNA-ribosyltransferase subunit 1, ribosyltransferase, queuine transfer ribonucleate, TGT, transfer ribonucleate glycosyltransferase, tRNA guanine transglycosidase, tRNA guanine transglycosylase, tRNA transglycosylase, tRNA-guanine 34 transglycosylase, tRNA-guanine transglycosylase, tRNA�guanine transglycosylase, virulence-associated protein VACC

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.2 Pentosyltransferases

                2.4.2.29 tRNA-guanosine34 preQ1 transglycosylase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
34820	AA Sequence
1	Application
1	CAS Registry Number
8	Cloned(Commentary)
10	Crystallization (Commentary)
65	Disease/ Diagnostics
2	General Information
109	Inhibitors
4	kcat/KM [mM/s]
79	Ki Value [mM]
36	KM Value [mM]
2	Localization
2	Metals/Ions
14	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
39	Organism
2	Pathway
213	PDB ID
1	pH Optimum
1	pH Range
12	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
38	Reference
8	Source Tissue
1	Specific Activity [micromol/min/mg]
34	Substrates and Products (Substrate)
5	Subunits
48	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
29	Turnover Number [1/s]

Engineering

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Engineering on EC 2.4.2.29 - tRNA-guanosine34 preQ1 transglycosylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Protein Variants Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D264N

Escherichia coli

-

is inactive and incapable of forming the covalent intermediate, while maintaining the ability to bind noncovalently to RNA

702263

D89A

Escherichia coli

-

less than 1% of the activity of the histidine-tagged wild-type enzyme

644933

D89C

Escherichia coli

-

less than 1% of the activity of the histidine-tagged wild-type enzyme

644933

D89E

Escherichia coli

-

about 50% of the activity of the histidine-tagged wild-type enzyme

644933

D89N

Escherichia coli

-

less than 1% of the activity of the histidine-tagged wild-type enzyme

644933

S90A

Escherichia coli

-

activity of the mutant enzyme is to low to determine Vmax and Km-value

644934

S90C

Escherichia coli

-

30fold increase in Km-value for tRNATyr and 4fold increase in Km-value for guanine

644934

S90F

Escherichia coli

-

mutant enzyme has no detectable solubility and reduced solubility

644934

E235Q

Zymomonas mobilis

P28720

the mutation has no significant influence on kcat, but Km for preQ1 is drastically increased, while Km for preQ0 seems to be decreased

688390

K52M

Zymomonas mobilis

P28720

reduced turnover value. At a concentration of protein of 0.01 mM appears almost exclusively as a homodimer as the wild-type, when the concentration of protein is lowered to a minimal value of 0.001 mM, a substantial proportion of monomer becomes evident

705164

Y106F

Zymomonas mobilis

P28720

crystallises similarly to the wild-type

703226

Y330F

Zymomonas mobilis

P28720

reduced turnover value. At a concentration of protein of 0.01 mM reveals a significant amount of monomer, when the concentration of protein is lowered to a minimal value of 0.001 mM, a substantial proportion of monomer becomes evident

705164