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6.4
2'-chloro-2'-deoxyadenosine
-
at pH 7.4 and 37°C
0.04
2'-deoxy-2'-azido-5'-deoxy-5'-methylthioadenosine
-
-
0.0004
2'-deoxy-2-fluoro-5'-deoxy-5'-methylthioadenosine
-
-
0.28
2'-deoxy-5'-deoxy-5'-methylthioadenosine
-
-
48.44 - 441.2
2'-deoxyadenosine
5.4
2'-fluoro-2'-deoxyadenosine
-
at pH 7.4 and 37°C
0.004
3'-deoxy-5'-deoxy-5'-methylthioadenosine
-
-
3.8
5'-deoxy-5'-(1,3-thiazol)-2-yl-sulfanyladenosine
-
-
2.14
5'-deoxy-5'-(2-(pyridin-2-yl)ethyl)-thioadenosine
-
-
5.5
5'-deoxy-5'-(2-methylbutyl)-thioadenosine
-
-
2.4
5'-deoxy-5'-(2-methylphenyl)-thioadenosine
-
-
1.4
5'-deoxy-5'-(3-methylphenyl)-thioadenosine
-
-
1.5
5'-deoxy-5'-(4-methoxyphenyl)-thioadenosine
-
-
0.3
5'-deoxy-5'-(4-methylphenyl)-thioadenosine
-
-
2.5
5'-deoxy-5'-(phenylethyl)-thioadenosine
-
-
2.49
5'-deoxy-5'-(phenylmethyl)-thioadenosine
-
-
3.44
5'-deoxy-5'-(quinolin-2-yl)-sulfanyladenosine
-
-
2.3
5'-deoxy-5'-butylthioadenosine
-
-
1.01
5'-deoxy-5'-cyclohexyl-thioadenosine
-
-
1.57
5'-deoxy-5'-cyclopentyl-thioadenosine
-
-
0.67
5'-deoxy-5'-ethylthioadenosine
-
-
5.9
5'-deoxy-5'-hydroxyethylthioadenosine
-
-
1.5
5'-deoxy-5'-imidazol-2-yl-sulfanyladenosine
-
-
2.9
5'-deoxy-5'-isobutylthioadenosine
-
-
0.85
5'-deoxy-5'-isopentylthioadenosine
-
-
4.6
5'-deoxy-5'-methyl-thioadenosine
-
-
84.62 - 443.8
5'-deoxy-5'-methylthioadenosine
3.16
5'-deoxy-5'-phenyl-thioadenosine
-
-
0.23
5'-deoxy-5'-pyridin-2-yl-sulfanyladenosine
-
-
4.2
5'-deoxy-5'-pyridin-4-yl-sulfanyladenosine
-
-
1.46
5'-deoxy-5'-tert-butylthioadenosine
-
-
0.8
5'-deoxy-5'-[4-(trifluoromethyl)phenyl]-thioadenosine
-
-
4.8
5'-deoxy-adenosine
-
-
0.4 - 24.5
5'-methylthioadenosine
9.6
deoxyadenosine
-
at pH 7.4 and 37°C
0.26
phosphate
pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine
0.51 - 8.8
S-methyl-5'-thioadenosine
48.44
2'-deoxyadenosine
mutant enzyme S12T, at pH 7.4 and 25°C
80.03
2'-deoxyadenosine
mutant enzyme Q289L, at pH 7.4 and 25°C
224.3
2'-deoxyadenosine
wild type enzyme, at pH 7.4 and 25°C
299.2
2'-deoxyadenosine
mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C
372.8
2'-deoxyadenosine
mutant enzyme S12T/N87T, at pH 7.4 and 25°C
441.2
2'-deoxyadenosine
mutant enzyme N87T, at pH 7.4 and 25°C
84.62
5'-deoxy-5'-methylthioadenosine
mutant enzyme N87T, at pH 7.4 and 25°C
145.1
5'-deoxy-5'-methylthioadenosine
mutant enzyme S12T, at pH 7.4 and 25°C
241.6
5'-deoxy-5'-methylthioadenosine
mutant enzyme Q289L, at pH 7.4 and 25°C
253.9
5'-deoxy-5'-methylthioadenosine
mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C
416.2
5'-deoxy-5'-methylthioadenosine
mutant enzyme S12T/N87T, at pH 7.4 and 25°C
443.8
5'-deoxy-5'-methylthioadenosine
wild type enzyme, at pH 7.4 and 25°C
0.4
5'-methylthioadenosine
pH and temperature not specified in the publication, co-substrate: 50 mM phosphate
2
5'-methylthioadenosine
-
18.7
5'-methylthioadenosine
-
21.4
5'-methylthioadenosine
-
pH 7.4, 70°C
24.5
5'-methylthioadenosine
-
-
8.4
adenosine
-
at pH 7.4 and 37°C
43.6
adenosine
-
pH 7.4, 70°C
62.63
adenosine
mutant enzyme N87T, at pH 7.4 and 25°C
95.79
adenosine
mutant enzyme Q289L, at pH 7.4 and 25°C
98.42
adenosine
mutant enzyme S12T, at pH 7.4 and 25°C
280.3
adenosine
mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C
308.9
adenosine
mutant enzyme S12T/N87T, at pH 7.4 and 25°C
334.1
adenosine
wild type enzyme, at pH 7.4 and 25°C
7.31
guanosine
-
-
29.6
guanosine
-
pH 7.4, 70°C
9.38
Inosine
-
-
132
Inosine
-
pH 7.4, 70°C
0.51
S-methyl-5'-thioadenosine
mutant enzyme M144A, at pH 7.0 and 25°C
0.58
S-methyl-5'-thioadenosine
mutant enzyme S167A, at pH 7.0 and 25°C
0.58
S-methyl-5'-thioadenosine
mutant enzyme W179F, at pH 7.0 and 25°C
0.69
S-methyl-5'-thioadenosine
mutant enzyme W179A, at pH 7.0 and 25°C
0.71
S-methyl-5'-thioadenosine
mutant enzyme Y134F, at pH 7.0 and 25°C
0.78
S-methyl-5'-thioadenosine
mutant enzyme Y134A, at pH 7.0 and 25°C
1.3
S-methyl-5'-thioadenosine
mutant enzyme R85H, at pH 7.0 and 25°C
1.4
S-methyl-5'-thioadenosine
mutant enzyme R85A, at pH 7.0 and 25°C
1.8
S-methyl-5'-thioadenosine
mutant enzyme A15M, at pH 7.0 and 25°C
2.13
S-methyl-5'-thioadenosine
-
at pH 7.4 and 37°C
2.5
S-methyl-5'-thioadenosine
mutant enzyme V34I, at pH 7.0 and 25°C
2.7
S-methyl-5'-thioadenosine
mutant enzyme V34A, at pH 7.0 and 25°C
8
S-methyl-5'-thioadenosine
mutant enzyme R164A, at pH 7.0 and 25°C
8.8
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.0 and 25°C