2.4.2.28: S-methyl-5'-thioadenosine phosphorylase

This is an abbreviated version!
For detailed information about S-methyl-5'-thioadenosine phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.28

2.4.2.28

purine

polyamine

salvage

mtap-deficient

cdkn2a

mesothelioma

phosphorolysis

mesothelial

nucleosidase

codeleted

l-alanosine

co-deletion

mtap-deleted

phosphorylase-deficient

p15ink4b

5'-deoxyadenosine

5-methylthioribose

mtans

p14arf

enzyme-deficient

diagnostics

drug development

medicine

analysis

Reaction

S-methyl-5-thio-alpha-D-ribose 1-phosphate

Synonyms

5'-deoxy-5'-methylthioadenosine phosphorylase, 5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-deoxy-5'-methylthioadenosine:orthophosphate methylthioribosyltransferase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine phosphorylase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5-methylthioadenosine phosphorylase, 5�-methylthioadenosine phosphorylase, MeSAdo phosphorylase, MeSAdo/Ado phosphorylase, methylthioadenosine nucleosidase, methylthioadenosine nucleoside phosphorylase, methylthioadenosine phosphorylase, MTA phosphorylase, MTAN, MTAN1, MTAN2, MTAP, Mtap protein, MTAPase, MTN1, MTN2, PfMTAP, phosphorylase, methylthioadenosine, RSFP, Rv0535, SsMTAP, SsMTAP II, SsMTAPII, SSO2343

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.2 Pentosyltransferases

2.4.2.28 S-methyl-5'-thioadenosine phosphorylase

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Results	in table
12	Activating Compound
9873	AA Sequence
10	Application
1	CAS Registry Number
33	Cloned(Commentary)
15	Crystallization (Commentary)
738	Disease/ Diagnostics
3	Expression
13	General Information
13	General Stability
10	IC50 Value
146	Inhibitors
38	kcat/KM [mM/s]
68	Ki Value [mM]
119	KM Value [mM]
7	Localization
3	Metals/Ions
35	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
19	Organic Solvent Stability
267	Organism
1	Oxidation Stability
6	Pathway
177	PDB ID
22	pH Optimum
5	pH Range
1	pH Stability
2	pI Value
53	Protein Variants
39	Purification (Commentary)
16	Reaction
1	Reaction Type
92	Reference
1	Renatured (Commentary)
116	Source Tissue
49	Specific Activity [micromol/min/mg]
8	Storage Stability
229	Substrates and Products (Substrate)
40	Subunits
130	Synonyms
1	Systematic Name
36	Temperature Optimum [°C]
10	Temperature Range [°C]
54	Temperature Stability [°C]
77	Turnover Number [1/s]

Purification

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Purification on EC 2.4.2.28 - S-methyl-5'-thioadenosine phosphorylase

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9 entries

as a GST fusion form, by gel filtration

Arabidopsis thaliana

Q9T0I8

700813

by centrifugation and on Ni-NTA column and by gel filtration

Arabidopsis thaliana

Q7XA67, Q9T0I8

681477

by ultrasonication, centrifugation, gel filtration and anion exchange chromatography, to homogeneity

Arabidopsis thaliana

Q7XA67, Q9T0I8

702045

Co-NTA agarose column chromatography

Schistosoma mansoni

I0B503

760012

Fractogel EMD DEAE column chromatograph and Resource Q column chromatography

from lung

Ni-NTA column chromatography

Homo sapiens

Q13126

735675

Ni-NTA column chromatography and Sephadex G-25 gel filtration

Trypanosoma brucei

736501

Ni-NTA resin column chromatography

Homo sapiens

758561

Ni2+-Sepharose column chromatography

2 entries

nickel affinity column chromatography, and Superdex 200 gel filtration

on Ni-NTA column

partial

recombinant enzyme

recombinant from Escherichia coli

4 entries

recombinant His-tagged enzyme from Escherichia coli

Homo sapiens

637551

using heat-treatment and MTA-Sepharose chromatography

Saccharolobus solfataricus

721732