2.4.2.28: S-methyl-5'-thioadenosine phosphorylase

This is an abbreviated version!
For detailed information about S-methyl-5'-thioadenosine phosphorylase, go to the full flat file.

Reaction

Synonyms

5'-deoxy-5'-methylthioadenosine phosphorylase, 5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-deoxy-5'-methylthioadenosine:orthophosphate methylthioribosyltransferase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine phosphorylase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5-methylthioadenosine phosphorylase, 5�-methylthioadenosine phosphorylase, MeSAdo phosphorylase, MeSAdo/Ado phosphorylase, methylthioadenosine nucleosidase, methylthioadenosine nucleoside phosphorylase, methylthioadenosine phosphorylase, MTA phosphorylase, MTAN, MTAN1, MTAN2, MTAP, Mtap protein, MTAPase, MTN1, MTN2, PfMTAP, phosphorylase, methylthioadenosine, RSFP, Rv0535, SsMTAP, SsMTAP II, SsMTAPII, SSO2343

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.2 Pentosyltransferases

                2.4.2.28 S-methyl-5'-thioadenosine phosphorylase

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Results	in table
12	Activating Compound
9873	AA Sequence
10	Application
1	CAS Registry Number
33	Cloned(Commentary)
15	Crystallization (Commentary)
738	Disease/ Diagnostics
3	Expression
13	General Information
13	General Stability
10	IC50 Value
146	Inhibitors
38	kcat/KM [mM/s]
68	Ki Value [mM]
119	KM Value [mM]
7	Localization
3	Metals/Ions
35	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
19	Organic Solvent Stability
267	Organism
1	Oxidation Stability
6	Pathway
177	PDB ID
22	pH Optimum
5	pH Range
1	pH Stability
2	pI Value
53	Protein Variants
39	Purification (Commentary)
16	Reaction
1	Reaction Type
92	Reference
1	Renatured (Commentary)
116	Source Tissue
49	Specific Activity [micromol/min/mg]
8	Storage Stability
229	Substrates and Products (Substrate)
40	Subunits
130	Synonyms
1	Systematic Name
36	Temperature Optimum [°C]
10	Temperature Range [°C]
54	Temperature Stability [°C]
77	Turnover Number [1/s]

Organic Solvent Stability

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Organic Solvent Stability on EC 2.4.2.28 - S-methyl-5'-thioadenosine phosphorylase

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ORGANIC SOLVENT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

1-propanol

2 entries

acetonitrile

2 entries

dimethylformamide

2 entries

Ethanol

3 entries

guanidine-HCl

Saccharolobus solfataricus

P50389

4 M, 24 h at room temperature, no loss of activity

637544

Methanol

3 entries

N,N-dimethylformamide

Saccharolobus solfataricus

P50389

50%, 24 h at room temperature, no loss of activity

637544

SDS

Saccharolobus solfataricus

P50389

0.075%, 24 h at room temperature, no loss of activity. After 60 min of incubation in 2% SDS the enzyme remains fully active at 70°C and retains 20% residual activity at 90°C. Complete inactivation after 5 min at 100°C in 0.5% SDS

637544

tetrahydrofuran

2 entries

Triton X-100

Saccharolobus solfataricus

P50389

9 M, 24 h at room temperature, no loss of activity

637544

urea

Saccharolobus solfataricus

P50389

9 M, 24 h at room temperature, no loss of activity

637544

1-propanol

Pyrococcus furiosus

-

at 50%: 88% remaining activity after 60 min at 50°C, 58% remaining activity after 60 min at 70°C, 22% remaining activity after 60 min at 90°C

637556

1-propanol

Saccharolobus solfataricus

P50389

no loss of activity after 24 h at room temperature in presence of 50% methanol, loss of 90% activity at 90°C after 1 h

637544

acetonitrile

Pyrococcus furiosus

-

at 50%: stable at 90°C for 30 min, loss of 62% activity after 60 min

637556

acetonitrile

Saccharolobus solfataricus

P50389

loss of 89% activity at 70°C after 1 h

637544

dimethylformamide

Pyrococcus furiosus

-

at 50%: 48% remaining activity after 60 min at 50°C, 5% remaining activity after 60 min at 70°C, no activity at 90°C

637556

dimethylformamide

Saccharolobus solfataricus

P50389

no loss of activity after 24 h at room temperature in presence of 50% dimethylformamide, loss of 21% activity at 90°C after 1 h

637544

Ethanol

Pyrococcus furiosus

-

at 50%: 90% remaining activity after 60 min at 50°C, 65% remaining activity after 60 min at 70°C, 30% remaining activity after 60 min at 90°C

637556

Ethanol

Saccharolobus solfataricus

P50389

no loss of activity after 24 h at room temperature in presence of 50% ethanol, loss of 88% activity at 90°C after 1 h

637544

Ethanol

Saccharolobus solfataricus

P50389

50%, 24 h at room temperature, no loss of activity

637544

Methanol

Pyrococcus furiosus

-

at 50%: stable at 90°C for 30 min, loss of 77% activity after 60 min

637556

Methanol

Saccharolobus solfataricus

P50389

no loss of activity after 24 h at room temperature in presence of 50% methanol, loss of 80% activity at 90°C after 1 h

637544

Methanol

Saccharolobus solfataricus

P50389

50%, 24 h at room temperature, no loss of activity

637544

tetrahydrofuran

Pyrococcus furiosus

-

at 50%: 77% remaining activity after 60 min at 50°C, 50% remaining activity after 60 min at 70°C, 3% remaining activity after 60 min at 90°C

637556

tetrahydrofuran

Saccharolobus solfataricus

P50389

loss of 76% activity at 70°C after 30 min, complete loss of activity after 1 h at 70°C

637544