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2.4.2.28: S-methyl-5'-thioadenosine phosphorylase

This is an abbreviated version!
For detailed information about S-methyl-5'-thioadenosine phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.28

Reaction

S-methyl-5'-thioadenosine
+
phosphate
=
adenine
 +
S-methyl-5-thio-alpha-D-ribose 1-phosphate

Synonyms

5'-deoxy-5'-methylthioadenosine phosphorylase, 5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-deoxy-5'-methylthioadenosine:orthophosphate methylthioribosyltransferase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine phosphorylase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5-methylthioadenosine phosphorylase, 5’-methylthioadenosine phosphorylase, MeSAdo phosphorylase, MeSAdo/Ado phosphorylase, methylthioadenosine nucleosidase, methylthioadenosine nucleoside phosphorylase, methylthioadenosine phosphorylase, MTA phosphorylase, MTAN, MTAN1, MTAN2, MTAP, Mtap protein, MTAPase, MTN1, MTN2, PfMTAP, phosphorylase, methylthioadenosine, RSFP, Rv0535, SsMTAP, SsMTAP II, SsMTAPII, SSO2343

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.28 S-methyl-5'-thioadenosine phosphorylase

Cloned

Cloned on EC 2.4.2.28 - S-methyl-5'-thioadenosine phosphorylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
different regions of MTAN cloned into the pGBT9.BS vector and transformed into Y190 yeast cells carrying pGAD.GH or pGAD.CBL3. Expressed in Escherichia coli BL21 cells carrying a GST fusion construct. MTAN promoter-GUS construct (pBI.tMTAN) transformed into Agrobacterium tumefaciens strain GV3101 and introduced into Arabidopsis plants by the floral dip method. 35S::CFP-CBL3-Myc and 35S::YFP-MTANHA constructs introduced, either alone or in combination, into Nicotiana benthamiana leaves by Agrobacterium tumefaciens-mediated infiltration method. CBL3-GFP and MTAN-GFP chimeric genes under the control of the cauliflower mosaic virus 35S promoter (pMD.CBL3 and pMD.MTAN) transiently expressed in Allium cepa epidermal cells. Onion epidermal cells bombarded with fusion constructs CBL3-YN and MTAN-YC
expressed in cutaneous squamous cell carcinoma cells
-
expressed in Drosophila melanogaster and Mus musculus
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells
expression at a high level in Escherichia coli
expression in Escherichia coli
-
expression in Escherichichia coli
expression of His-tagged protein in Escherichia coli BL21(DE3) cells
-
expression of MTAP in MTAP-deleted MCF-7 breast adenocarcinoma cells results in a significant reduction of ODC activity
-
expression of the N-terminally His6-tagged enzyme in Escherichia coli
-
from cells of patients with T-cell acute lymphoblastic leukemia, DNA sequence analysis, 9p21 chromosome, topologic map
functional expression in enzyme deficient cell line MCF-7
-
gene MTAP, located f100 kb telomeric to the p16INK4A/CDKN2A gene on chromosome 9p21, expression in HuCCT1 and SNU308 cell lines, the enzyme expression does not affect the cells
-
into pGEX-4T3 vector and overexpressed in Escherichia coli BL21 (DE3) RIL
into the pET28a+ vector at the NdeI and HindIII sites, overexpression of the enzyme with a cleavable N-terminal His6 tag in Escherichia coli BL21 Codon+ cells
overexpression in Escherichia coli BL21
-
overexpression in Escherichia coli strain RB791, amino acid determination, incorrect positioning of disulfide bonds, the recombinant enzyme is less thermostable and thermophilic than the native enzyme
quantitative PCR, RNA mutational analysis, analysis of methylation status of the enzymes promotor
-
re-expression in Mel Im cell line
-
recombinant SsMTAPII and its C262S and C259S/C261S mutant forms are expressed in Escherichia coli BL21 (lDE3) cells
-
RRK081 embryonic stem cells, containing a gene-trap vector inserted between exon 3 and exon 4 of the mouse Mtap locus (MtaplacZ) injected into mouse blastocysts derived from C57BL/6 animals, embryos implanted in pseudopregnant females
-
the gene maps on the 9p21 chromosome, strictly linked to the tumor suppressor gene p16INK4A, functional expression in Escherichia coli strain JM105
-
wild-type and promotor deletion mutant, expression in HeLa cells, luciferase reporter assay
-