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2.4.2.26: protein xylosyltransferase

This is an abbreviated version!
For detailed information about protein xylosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.26

Reaction

UDP-alpha-D-xylose
+
[protein]-L-serine
=
UDP
+
[protein]-3-O-(beta-D-xylosyl)-L-serine

Synonyms

LARGE, More, peptide O-xylosyltransferase, peptide O-xylosyltransferase 1, peptide-O-xylosyltransferase, Rumi, squashed vulva protein 6, SQV-6, UDP-alpha-D-xyloase:proteoglycan core protein beta-D-xylosyltransferase, UDP-alpha-D-xylose:proteoglycan core protein beta-D-xylosyltransferase, UDP-D-xylose:core protein beta-D-xylosyltransferase, UDP-D-xylose:core protein xylosyltransferase, UDP-D-xylose:proteoglycan core protein b-d-xylosyltransferase, UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase, UDP-xylose-core protein beta-D-xylosyltransferase, uridine diphosphoxylose-core protein beta-xylosyltransferase, uridine diphosphoxylose-protein xylosyltransferase, XT, XT-I, XT-II, xylosyltransferase, xylosyltransferase 1, xylosyltransferase 2, xylosyltransferase I, xylosyltransferase II, xylosyltransferase sqv-6, xylosyltransferase, uridine diphosphoxylose-core protein beta-, xylosyltransferase-I, xylosyltransferases II, XylT-I, XylT-II, XYLT1, XYLT2

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.26 protein xylosyltransferase

Cloned

Cloned on EC 2.4.2.26 - protein xylosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
5' and 3' deletion constructs expressed in SW1353 cells
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c-Myc/TGFalpha doubly transgenic mice. Modified minigene Xylt2 expression construct producing a protein lacking the 45 N-terminal amino acids including the transmembrane domains. The Xylt1 expression cassette producing a protein that lacks 94 N-terminal amino acids including the transmembrane domain which is predicted as for XylT2. Both Xylt1 and Xylt2 expression cassettes cloned into a modified pcDNA3.1 vector containing a transferrin signal peptide and an HPC4 epitope tag in frame on the N-terminus
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DNA sequence determination and analysis of isoforms XT-I and XT-II, chromosome mapping
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DNA sequence determination and analysis, chromosome mapping, functional expression of isoforms XT-I and XT-II in CHO-K1 cells, no activity when XT-II is expressed fused to the aminoterminal peptide tag, 8% of the recombinant activity is located in the cytosol and membranes, 92% recombinant activity is secreted into the medium
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expressed as glutathione-S-transferase fusion proteins containing putative or known GAG attachment sites of in vivo proteoglycans. Cloned into the XhoI and EcoRI digested pGEX-6P-1 vector and ligated by T4 DNA ligase. Expressed in Escherichia coli BL21 DE3 cells
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expressed in CHO cells, high five insect cells, HEK293 cells, and SaOS-2 cells, and in Pichia pastoris
expressed in Pichia pastoris strain X-33
expressed in the xylosyltransferase-deficient pgsA-745 (S745) CHO cell line and in Pichia pastoris
expressed in xylosyltransferase-deficient pgsA-745 CHO cells
expression in Pichia pastoris
expression in Pichia pastoris. Soluble form of human XT-II is expressed in the xylosyltransferase-deficient pgsA-745 (S745) Chinese hamster ovary cell line
expression in Pichia pastoris. Transfection of the xylosyltransferase-deficient Chinese hamster ovary mutant pgsA-745 with XT-II coding cDNA completely restores glycosaminoglycan biosynthesis
expression of various GFP-tagged XT-II mutants with C-terminal truncations and deletions in HEK-293 and SaOS-2 cells
expression of XT-I-GFP and various GFP-tagged XT-I mutants with C-terminal truncations and deletions in HEK-293 and SaOS-2 cells
high-level expression in High Five insect cells
-
inducible expression in Pichia pastoris
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into pGEX-6P-1, ligated into the multiple cloning site of the BamHI-digested and shrimp alkaline phosphatase-dephosphorylated pMAL-c4E expression vector, in frame with the N-terminal located maltose binding protein, expressed in Escherichia coli strain BL21(DE3) under the control of the IPTG inducible tac promoter. MBP/XylT-II fusion protein expressed in ER2507 that contains prolonged XylT-II fragments (MBP/XylT-IIF1-3 and MBP/XylT-IIF4-6)
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soluble active form of human XT-I is expressed in High Five insect cells, Trichoplusia ni
soluble mutant enzymes of human XT-I with deletions at the N-terminal domain are expressed in insect cells
-
XylT-II cloned into the multiple cloning site of the pPICZ alphaA expression vector, in frame with the alpha-factor signal sequence and with a C-terminal located myc-epitope and hexa-histidine tag, expressed in Pichia pastoris strain X-33
-