2.4.2.2: pyrimidine-nucleoside phosphorylase
This is an abbreviated version!
For detailed information about pyrimidine-nucleoside phosphorylase, go to the full flat file.
Reaction
Synonyms
AmPyNP, BbPyNP, BfPyNP, BsPyNP, deoA, EC 2.4.2.23, GsPyNP, GtPyNP, LpPyNP, nucleoside phosphorylase, PDP, phosphorylase, pyrimidine nucleoside, Py-NPase, PYNP, pynpase, pyrimidine nucleoside phosphorylase, pyrimidine ribonucleoside phosphorylase, pyrimidine-nucleoside phosphorylase, pyrimidine/purine nucleoside phosphorylase, TTHA1771, TtPyNP, udp, UPase
ECTree
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Temperature Stability
Temperature Stability on EC 2.4.2.2 - pyrimidine-nucleoside phosphorylase
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40
purified recombinant enzyme, pH 8.5, 1 h, 90% activity remaining
45
purified recombinant enzyme, pH 8.5, 1 h, 80% activity remaining
50
55
purified recombinant enzyme, pH 8.5, 1 h, 70% activity remaining
60
70
80
purified recombinant enzyme, pH 8.5, 1 h, 65.6% activity remaining
50
purified recombinant enzyme, pH 8.5, 1 h, 75% activity remaining
50
purified recombinant enzyme, pH 8.5, 1 h, 43.7% activity remaining
50
purified recombinant enzyme, pH 8.5, 1 h, 52.1% activity remaining
purified recombinant enzyme, pH 8.5, 1 h, 10% activity remaining
-
half-life: 25 min in 20 mM potassium phosphate, 15.1 h in 20 mM potassium phosphate and 1 mM 5-methyluridine
70
purified enzyme, half-life is 23 min in the presence of 20 mM potassium phosphate and 1 mM inosine, and 15.1 h in the presence of 20 mM potassium phosphate and 1 mM 5-methyluridine