2.4.2.2: pyrimidine-nucleoside phosphorylase
This is an abbreviated version!
For detailed information about pyrimidine-nucleoside phosphorylase, go to the full flat file.
Word Map on EC 2.4.2.2
Reaction
Synonyms
AmPyNP, BbPyNP, BfPyNP, BsPyNP, deoA, EC 2.4.2.23, GsPyNP, GtPyNP, LpPyNP, nucleoside phosphorylase, PDP, phosphorylase, pyrimidine nucleoside, Py-NPase, PYNP, pynpase, pyrimidine nucleoside phosphorylase, pyrimidine ribonucleoside phosphorylase, pyrimidine-nucleoside phosphorylase, pyrimidine/purine nucleoside phosphorylase, TTHA1771, TtPyNP, udp, UPase
ECTree
2.4.2.2 pyrimidine-nucleoside phosphorylaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.4.2.2 - pyrimidine-nucleoside phosphorylase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate
1,2,4-triazole-3-carboxamide + alpha-D-ribose 1-phosphate
ribavirin + phosphate
-
-
-
-
?
2'-C-methyluridine + phosphate
uracil + 2-C-methyl-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
2'-deoxy-2'-fluorouridine + phosphate
?
-
-
poor activity, 0.4% substrate conversion after 30 min
-
?
2'-deoxy-2'-fluorouridine + phosphate
uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
2'-deoxyuridine + 5-bromovinyluracil
(E)-5-(2-bromovinyl)-2'-deoxyuridine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-fluorouracil
5-fluoro-2'-deoxyuridine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-trifluoromethyluracil
5-trifluoromethyl-2'-deoxyuridine + uracil
-
-
-
-
?
2,6-dichloropurine 2'-deoxyriboside + phosphate
?
-
-
-
r
3'-C-methyluridine + phosphate
uracil + 3-C-methyl-alpha-D-ribose 1-phosphate
-
-
-
r
3'-fluoro-3'-deoxyuridine + phosphate
uracil + 3'-fluoro-3'-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
4-thiothymidine + phosphate
4-thiothymine + alpha-D-ribose 1-phosphate
-
uridine phosphorylase activity, see also EC 2.4.2.3
-
-
r
5'-azido-5'-deoxythymidine + phosphate
thymine + 5-azido-5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5'-bromo-5'-deoxythymidine + phosphate
thymine + 5-bromo-5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5'-chloro-5'-deoxythymidine + phosphate
thymine + 5'-chloro-5'-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5'-deoxy-5'-methyleneuridine + phosphate
uracil + 5-deoxy-5'-methylene-alpha-D-ribose 1-phosphate
-
-
-
r
5'-deoxy-5-fluorouridine + phosphate
5-fluorouracil + alpha-D-deoxyribose 1-phosphate
-
-
5-fluorouracil is a chemotherapeutic agent and inactivated by dihydropyrimidine dihydrogenase activity
-
?
5'-deoxythymidine + phosphate
thymine + 5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5'-homothymidine + phosphate
thymine + 5-homo-alpha-D-ribose 1-phosphate
-
-
-
r
5'-homouridine + phosphate
uracil + 5-homo-alpha-D-ribose 1-phosphate
-
-
-
r
5'-iodo-5'-deoxythymidine + phosphate
thymine + 5-iodo-5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5-bromodeoxyuridine + phosphate
5-bromouracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
5-C-methyluridine + phosphate
5-C-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-chlorodeoxyuridine + phosphate
5-chlorouracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
5-fluorodeoxyuridine + phosphate
5-fluorouracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-iododeoxyuridine + phosphate
5-iodouracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
5-iodouridine + phosphate
5-iodouracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-trifluoromethyl-2'-deoxyuridine + phosphate
5-trifluoromethyluracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
6-chloro-2-fluoropurine 2'-deoxyriboside + phosphate
?
-
-
-
r
6-methyluridine + phosphate
6-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
alpha-D-ribose 1-phosphate + uracil
phosphate + uridine
-
-
-
?
phosphate + uridine
alpha-D-ribose 1-phosphate + uracil
-
-
-
r
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
thymidine + phosphate
-
-
-
r
uracil arabinoside + phosphate
uracil + alpha-D-arabinose 1-phosphate
-
-
-
-
?
(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate
-
1.6% substrate conversion after 30 min
-
?
(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
1.6% substrate conversion after 30 min
-
?
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
-
-
r
2'-deoxy-2'-fluorouridine + phosphate
uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate
-
15.6% substrate conversion after 30 min
-
?
2'-deoxy-2'-fluorouridine + phosphate
uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
15.6% substrate conversion after 30 min
-
?
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
-
-
-
r
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
4-thiouridine + phosphate
4-thiouracil + alpha-D-ribose 1-phosphate
-
-
-
r
4-thiouridine + phosphate
4-thiouracil + alpha-D-ribose 1-phosphate
-
thymidine phosphorylase activity, see also EC 2.4.2.4
-
-
r
5'-deoxyuridine + phosphate
uracil + 5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
5'-deoxyuridine + phosphate
uracil + 5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromodeoxyuridine + phosphate
5-bromouracil + deoxyribose 1-phosphate
-
95% of the activity with uridine
-
-
?
5-bromodeoxyuridine + phosphate
5-bromouracil + deoxyribose 1-phosphate
-
-
-
-
?
5-bromodeoxyuridine + phosphate
5-bromouracil + deoxyribose 1-phosphate
-
74% of the activity with uridine
-
-
?
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
-
95% of the activity with uridine
-
-
?
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
-
40% of the activity with uridine
-
-
?
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
27% of the activity with uridine
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-trifluorothymidine + phosphate
5-trifluorothymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
5-trifluorothymidine + phosphate
5-trifluorothymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
cytidine + phosphate
cytosine + alpha-D-ribose 1-phosphate
-
8% of the activity with uridine
-
-
?
cytidine + phosphate
cytosine + alpha-D-ribose 1-phosphate
-
no activity
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
2'-deoxyuridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
179% of the activity with uridine, by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed
-
-
r
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
2'-deoxyuridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
12% of the activity with uridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
147% of the activity with uridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
147% of the activity with uridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
?
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
-
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
ribofuranosyl thymine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
-
?
ribofuranosyl thymine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
139% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
21% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
99% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
99% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-
?
thymidine + phosphate
thymine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-deoxyribose 1-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-
r
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
preferred substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
prefered substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
prefered substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
preferred substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
prefered substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
prefered substrate
-
-
?
additional information
?
-
the function of His82, Arg169, and Lys188 is to stabilize the uridine in a high-energy conformation by means of electrostatic interactions. These residues are involved in catalysis. The function of Asp162 is likely to activate Lys188 for phosphorolytic catalysis through polarization effects
-
-
?
additional information
?
-
-
the function of His82, Arg169, and Lys188 is to stabilize the uridine in a high-energy conformation by means of electrostatic interactions. These residues are involved in catalysis. The function of Asp162 is likely to activate Lys188 for phosphorolytic catalysis through polarization effects
-
-
?
additional information
?
-
-
the enzyme does not accept arabinonucleosides and 2',3'-dideoxynucleosides as substrates
-
-
?
additional information
?
-
pyrimidine-nucleoside phosphorylase catalyzes the phosphorolytic cleavage of thymidine and uridine with equal activity
-
-
-
additional information
?
-
-
pyrimidine-nucleoside phosphorylase catalyzes the phosphorolytic cleavage of thymidine and uridine with equal activity
-
-
-
additional information
?
-
under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine
-
-
-
additional information
?
-
-
under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine
-
-
-
additional information
?
-
under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine
-
-
-
additional information
?
-
-
predictive profiling of 5-fluorouracil resistance in human cancer cells, overview
-
-
?
additional information
?
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kinetic constants of the phosphorolysis reaction of uridine derivatives modified at 2'-, 3'- and 5'-positions of the sugar moiety and 2-, 4-, 5- and 6-positions of the heterocyclic base are determined. The absence of the 2'- or 5'-hydroxyl group is not crucial for successful binding and phosphorolysis. On the other hand, absence of both the 2'- and 5'-hydroxyl groups leads to loss of substrate binding to the enzyme. Substrate specificity, overview. 2,2'-O-Anhydrouridine, that models the high-syn conformation of uridine, binds better to the enzyme than uridine, despite the absence of a proton in the 3-position of the heterocyclic base, thus playing an important role in the substrate binding. In this case, the orientation of the heterocycle substantially outweighs the contribution of hydrogen bonding by the 2'-hydroxyl and 3-NH groups in the active site of enzyme UP. This strongly suggests that the 2-oxo group is located quite close to 2'-H in the substrate-UP complex. This is in line with the lack of substrate properties of 2-thiouridine
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additional information
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no activity with deoxycytidine
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additional information
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reversible phosphorolysis of pyrimidines in the nucleotide synthesis salvage pathway
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additional information
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thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
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additional information
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no substrate: cytidine, deoxycytidine, 2',2'-difluoro-2'-deoxycytidine, 2',2'-difluoro-2'-deoxyuridine, uracil arabinoside, thymine arabinoside, (E)-5-(2-bromovinyl)-deoxyuridine, azidothymidine, stavudine, 6-azauridine
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additional information
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the enzyme also displays (2'-deoxy)ribosyltransferase activity
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additional information
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the enzyme also displays (2'-deoxy)ribosyltransferase activity
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additional information
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thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
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additional information
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no substrate: cytidine, deoxycytidine, adenosine, inosine, or guanosine
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additional information
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no substrate: cytidine, deoxycytidine, adenosine, inosine, or guanosine
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additional information
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thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
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