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2.4.2.19: nicotinate-nucleotide diphosphorylase (carboxylating)

This is an abbreviated version!
For detailed information about nicotinate-nucleotide diphosphorylase (carboxylating), go to the full flat file.

Word Map on EC 2.4.2.19

Reaction

beta-nicotinate D-ribonucleotide
+
diphosphate
 +
CO2
=
pyridine-2,3-dicarboxylate
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

BNA6, general stress protein 70 , GSP70 , Hp-QAPRTase, hQPRTase, M6_Spy1061, NAD pyrophosphorylase, NadC, nicotinate mononucleotide pyrophosphorylase (carboxylating) (EC 2.4.2.19), nicotinate-nucleotide pyrophosphorylase (carboxylating), nicotinate-nucleotide:pyrophosphate phospho-alpha-D-ribosyltransferase (decarboxylating), pyrophosphorylase, nicotinate mononucleotide (carboxylating), QAPRTase, QPRT, QPRTase, QPRTase , QPT, QPT1, QPT2, quinolate phosphoribosyltransferase, quinolinate phosphoribosyl transferase, quinolinate phosphoribosyltansferase, quinolinate phosphoribosyltransferase, quinolinate phosphoribosyltransferase (decarboxylating), quinolinate phosphoribosyltransferase 1, quinolinate phosphoribosyltransferase 2, quinolinate phosphoribosyltransferase [decarboxylating] , quinolinic acid phosphoribosyl transferase, quinolinic acid phosphoribosyltransferase, quinolinic phosphoribosyltransferase, sp.NadC, spNadC, TM1645, type II quinolic acid phosphoribosyltransferase, type II quinolinic acid phosphoribosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.19 nicotinate-nucleotide diphosphorylase (carboxylating)

Cloned

Cloned on EC 2.4.2.19 - nicotinate-nucleotide diphosphorylase (carboxylating)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as glutathione S-transferase fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)-RIPL Codon Plus
expressed in Escherichia coli TH265 (nadC mutant)(complementation approach)
Q0WX54, Q0WX55
expression in Escherichia coli
gene TM1645, expression of N-terminally His-tagged enzyme in Escherichia coli
His-tagged protein expressed in Escherichia coli C41 (DE3)
-
in pEHISTEV (pBS-hQPRT as template) for expression in Escherichia coli BL21(DE3) or mutagenesis, expression with N-terminal hexa-His tag and TEV protease cleavage site, two extra N-terminal residues (Gly, Ala) remain after removal of hexa-His tag
into plasmid pTYB12 and expressed in Escherichia coli BL21
nadC coding sequence cloned into the pRSETC expression vector, overexpressed in Escherichia coli nadC-deleted host strain ZB100
-
overexpression in Escherichia coli
-
subcloned into a T7-based expression system
-