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2.4.2.17: ATP phosphoribosyltransferase

This is an abbreviated version!
For detailed information about ATP phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.17

Reaction

1-(5-phospho-beta-D-ribosyl)-ATP
+
diphosphate
=
ATP
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

1-(5-phospho-D-ribosyl)-ATP:pyrophosphate phospho-alpha-D-ribosyltransferase, adenosine 5'-triphosphate phosphoribosyltransferase, adenosine triphosphate phosphoribosyltransferase, adenosine-triphosphate phosphoribosyltransferase, ATP phosphoribosyl transferase, ATP phosphoribosyl transferase complex, ATP phosphoribosyltransferase, ATP-phosphoribosyl transferase, ATP-phosphoribosyltransferase, ATP-PRT, ATP-PRT1, ATP-PRT2, ATP-PRTase, ATPPRT, HisG, HisGL, N-1-(5'-phosphoribosyl)-ATP transferase, phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP pyrophosphorylase, phosphoribosyl-ATP:pyrophosphate-phosphoribosyl phosphotransferase, phosphoribosyladenosine triphosphate pyrophosphorylase, phosphoribosyladenosine triphosphate synthetase, phosphoribosyltransferase, adenosine triphosphate

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.17 ATP phosphoribosyltransferase

Purification

Purification on EC 2.4.2.17 - ATP phosphoribosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and DEAE-cellulose chromatography
-
centrifugation and DEAE-Sephacel anion-exchange column
-
first purification by heating the crude extract, ammonium sulfate precipitation and selective histidine-dependent ammonium sulfate precipitation
-
HisTrap column chromatography
HisTrap resin column chromatography and Superdex S200 gel filtration
HiTrap Talon column chromatography and Superdex 200 gel filtration
HiTrap Talon resin column chromatography, GST-affinity column chromatography and Superdex S200 gel filtration
immobilized metal ion affinity chromatography (Ni2+)
-
immobilized metal ion affinity chromatography, gel filtration
metal affinity column chromatography
metal affinity column chromatography and Sephacryl S-200 gel filtration
Mn2+ precipitation, DEAE-cellulose and ammonium sulfate precipitation, later on Sephadex G-150
-
nickel-nitrilotriacetic acid column, Sephadex G-200 later on
-
recombinant selenomethionine-labeled enzyme from strain B834(DE3)
recombinant wild-type and selenomethionine-labeled enzyme complex comprising subunits HisGs and HisZ from Escherichia coli
-
recombinant wild-type and selenomethionine-labeled subunits HisGs and HisZ from Escherichia coli in a multistep procedure
-
the recombinant enzymes are produced as fused proteins with a maltose-binding protein, and the purification is made by a two step amylose resin column followed by a digestion with Factor Xa