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2.4.2.14: amidophosphoribosyltransferase

This is an abbreviated version!
For detailed information about amidophosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.14

Reaction

5-phospho-beta-D-ribosylamine
+
diphosphate
+
L-glutamate
=
L-glutamine
+
5-phospho-alpha-D-ribose 1-diphosphate
+
H2O

Synonyms

5'-phosphoribosylpyrophosphate amidotransferase, 5-phosphoribosyl-1-pyrophosphate amidotransferase, 5-phosphoribosylpyrophosphate amidotransferase, 5-phosphororibosyl-1-pyrophosphate amidotransferase, ADE4, alpha-5-phosphoribosyl-1-pyrophosphate amidotransferase, amido phosphoribosyltransferase, amidotransferase, phosphoribosyl pyrophosphate, APRT, ATASE, AtGPRT, Gln phosphoribosylpyrophosphate amidotransferase, glutamine 5-phosphoribosylpyrophosphate amidotransferase, glutamine phosphoribosyl pyrophosphate amidotransferase, glutamine phosphoribosylpyrophosphate amidotransferase, glutamine ribosylpyrophosphate 5-phosphate amidotransferase, GPAT, GPATase, GPRAT, GPRAT2, phosphoribose pyrophosphate amidotransferase, phosphoribosyl amidotransferase, phosphoribosyl pyrophosphate amidotransferase, phosphoribosylamidotransferase, phosphoribosyldiphosphate 5-amidotransferase, phosphoribosylpyrophosphate glutamyl amidotransferase, PPAT, PRAT, PurF

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.14 amidophosphoribosyltransferase

Molecular Weight

Molecular Weight on EC 2.4.2.14 - amidophosphoribosyltransferase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
102000
-
sedimentation equilibrium centrifugation, phosphate, phosphoribosyldiphosphate or purine mononucleotides influence the sedimentation profile
110000
-
ligand-induced alteration of sedimentation coefficient and Stokes radius, gel filtration or sedimentation equilibrium centrifugation
127000
-
liver small form
133000
-
placenta small form, large form is converted to small form by incubation with phosphoribosyldiphosphate
170000
-
placenta large form, in the presence of AMP or GMP
172000
-
sedimentation equilibrium centrifugation in the presence of phosphoribosyldiphosphate, the sedimentation profile is influenced by phosphate, phosphoribosyldiphosphate or purine mononucleotides
180000
-
mutant unstable enzyme, gel filtration
181000
-
sedimentation equilibrium centrifugation in the presence of phosphate, the sedimentation profile is influenced by phosphate, phosphoribosyldiphosphate or purine mononucleotides
185000
-
highly concentrated enzyme solution, sucrose density gradient centrifugation, enzyme exists in equilibrium of tetramer, dimer and monomeric forms, conversion of dimer to tetramer within 10fold increase in protein concentration
194000
-
sedimentation equilibrium centrifugation
195000
-
sucrose density gradient centrifugation
200000
215000
224000
-
gel filtration
270000
-
placenta, large form, small form is converted to large form by incubation with purine nucleotides, large form presumably catalytically inactive
292000
-
liver, large form
360000
-
wild-type stable enzyme, gel filtration
50000
-
2-4 * 50000, SDS-PAGE
54000
SDS-PAGE analysis of the recombinant AtGPRAT2 expressed in Escherichia coli
55000
-
x * 55000, isoform PRAT3, SDS-PAGE
56395
-
3-4 * 56395, calculated from nucleotide sequence
56700
-
x * 56700, calculated
57000
-
3-4 * 57000, SDS-PAGE
58000
-
x * 58000, SDS-PAGE
8000000
-
probably an aggregation of several purine synthetic activities, gel filtration
93000
-
sucrose density gradient centrifugation, enzyme exists in equilibrium of tetrameric, dimeric and monomeric forms
additional information