2.4.2.14: amidophosphoribosyltransferase This is an abbreviated version! For detailed information about amidophosphoribosyltransferase, go to the full flat file .
Reaction
5-phospho-beta-D-ribosylamine +
diphosphate +
L-glutamate =
L-glutamine +
5-phospho-alpha-D-ribose 1-diphosphate +
H2O
Synonyms 5'-phosphoribosylpyrophosphate amidotransferase, 5-phosphoribosyl-1-pyrophosphate amidotransferase, 5-phosphoribosylpyrophosphate amidotransferase, 5-phosphororibosyl-1-pyrophosphate amidotransferase, ADE4, alpha-5-phosphoribosyl-1-pyrophosphate amidotransferase, amido phosphoribosyltransferase, amidotransferase, phosphoribosyl pyrophosphate, APRT, ATASE, AtGPRT, Gln phosphoribosylpyrophosphate amidotransferase, glutamine 5-phosphoribosylpyrophosphate amidotransferase, glutamine phosphoribosyl pyrophosphate amidotransferase, glutamine phosphoribosylpyrophosphate amidotransferase, glutamine ribosylpyrophosphate 5-phosphate amidotransferase, GPAT, GPATase, GPRAT, GPRAT2, phosphoribose pyrophosphate amidotransferase, phosphoribosyl amidotransferase, phosphoribosyl pyrophosphate amidotransferase, phosphoribosylamidotransferase, phosphoribosyldiphosphate 5-amidotransferase, phosphoribosylpyrophosphate glutamyl amidotransferase, PPAT, PRAT, PurF
ECTree
Metals Ions
Metals Ions on EC 2.4.2.14 - amidophosphoribosyltransferase
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Ca2+
-
activation, approx. 15% as effective as Mg2+ or Mn2+
Co2+
-
activation, approx. 40% as effective as Mg2+ or Mn2+
Mn2+
-
required, equally effective as Mg2+
Fe
-
-
Fe
-
low temperature magnetic circular dichroism, electron paramagnetic resonance and resonance Raman spectroscopy of oxidized and reduced [4Fe-4S] cluster, native enzyme contains an [4Fe-4S]2+ cluster
Fe
-
iron-sulfur center can be removed with 1,10-phenanthroline, resulting apoprotein is devoid of amino- and amidotransferase activity
Fe
-
required, iron-sulfur protein
Fe
-
enzyme contains a diamagnetic [4Fe-4S] cluster essential for activity
Fe
-
iron is oxidized by O2 to enzyme-bound Fe3+
Fe
-
enzyme contains a [4Fe-4S] cluster
Fe
-
enzyme contains a [4Fe-4S] cluster
Fe
-
enzyme contains a [4Fe-4S] cluster
Fe
-
loss of activity after oxidation of the iron-sulfur center
Fe
-
enzyme contains a [4Fe-4S] cluster
Mg2+
5 mM used in assay conditions
Mg2+
-
5-phospho-alpha-D-ribose 1-diphosphate-Mg3- is the reactive molecular species
Mg2+
-
Km-value: 0.65 mM, inhibition above 10 mM
Mg2+
-
maximal activation at concentrations 2.5-5 times higher than that of the corresponding 5-phospho-alpha-D-ribose 1-diphosphate concentration
additional information
-
-
additional information
-
4Fe-4S-cluster
additional information
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4Fe-4S-cluster
additional information
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4Fe-4S-cluster
additional information
-
low temperature magnetic circular dichroism, electron paramagnetic resonance and resonance Raman spectroscopy
additional information
-
S2- is oxidized by O2 to a mixture of sulfur oxides bound as thiocysteine and yet unidentified products
additional information
-
sulfur required, iron-sulfur protein
additional information
-
probably diamagnetic
additional information
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4Fe-4S-cluster
additional information
-
-
additional information
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prototype for a metal-free amidophosphoribosyltransferase
additional information
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non-heme iron is not present in significant amounts
additional information
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non-heme iron is not present in significant amounts
additional information
-
not activated by iron or sulfide
additional information
-
-
additional information
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4Fe-4S-cluster
additional information
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not activated by Ba2+, Cd2+, Cu2+, Fe2+, Hg2+, Ni2+, Zn2+
additional information
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4Fe-4S-cluster