2.4.1.B72: C-mannosyltransferase
This is an abbreviated version!
For detailed information about C-mannosyltransferase, go to the full flat file.
Word Map on EC 2.4.1.B72
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2.4.1.B72
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c-mannosylation
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elegans
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thrombospondin
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neuroblast
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acrosome
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round-headed
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netrin
- 2.4.1.B72
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c-mannosylation
- elegans
- thrombospondin
-
neuroblast
-
acrosome
-
round-headed
- netrin
Reaction
transfers a D-mannosyl residue from dolichyl D-mannosyl phosphate to the tryptophan residue of a protein acceptor forming a C-C bond =
Synonyms
C-mannosyltransferase, DPY-19, Dpy-19-like C-mannosyltransferase, Dpy-19-like protein 3, dpy19, DPY19L1, DPY19L3, DPY19L4, dumpy-19, Dumpy-19 protein homologue, Pf DPY-19, PF3D7_0806200, protein dpy-19 homolog 3, protein dumpy-19
ECTree
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Engineering
Engineering on EC 2.4.1.B72 - C-mannosyltransferase
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E206N
site-directed mutatgenesis, the mutation of DPY19L3, resulting in the N-glycosylation consensus sequence (206NFT208), does not induce N-glycosylation, confirming the region is certainly a cytoplasmic region
additional information
additional information
DPY19L3 has a splice variant, called isoform2, which lacks exons 16-19 and retains part of the intron sequence, resulting in a 542-amino-acid protein. Based on our topological model, isoform2 lacks the C-terminal luminal region of DPY19L3. Topological prediction of isoform2 is almost similar to wild-type DPY19L3, but the last transmembrane (TM) region of wild-type DPY19L3 is changed to re-entrant loop (RL), resulting in the C-terminus of isoform2 is predicted to localize cytoplasm. By redox-sensitive luciferase assay, the C-terminus of isoform2 has high Gaussia luciferase activity, as does this segment of DPY19L3, demonstrating the C-terminus of isoform2 is localized to ER lumen. Furthermore, isoform2 is localized in the ER similar to wild-type DPY19L3
additional information
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DPY19L3 has a splice variant, called isoform2, which lacks exons 16-19 and retains part of the intron sequence, resulting in a 542-amino-acid protein. Based on our topological model, isoform2 lacks the C-terminal luminal region of DPY19L3. Topological prediction of isoform2 is almost similar to wild-type DPY19L3, but the last transmembrane (TM) region of wild-type DPY19L3 is changed to re-entrant loop (RL), resulting in the C-terminus of isoform2 is predicted to localize cytoplasm. By redox-sensitive luciferase assay, the C-terminus of isoform2 has high Gaussia luciferase activity, as does this segment of DPY19L3, demonstrating the C-terminus of isoform2 is localized to ER lumen. Furthermore, isoform2 is localized in the ER similar to wild-type DPY19L3
additional information
CRISPR/Cas9 technology is used to generate a Pf DPY19 null mutant. Pf DPY19 gene disruption is not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding
additional information
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CRISPR/Cas9 technology is used to generate a Pf DPY19 null mutant. Pf DPY19 gene disruption is not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding