2.4.1.B52: mixed-linkage glucan endotransglucosylase
This is an abbreviated version!
For detailed information about mixed-linkage glucan endotransglucosylase, go to the full flat file.
Reaction
transfers a (1->3),(1->4) mixed-linkage glucan to a xyloglucan =
Synonyms
BdTHX8, cellulose:xyloglucan endotransglucosylase, CXE, FvXTH9, grass-specific glycoside hydrolase family 16 endotransglucosylase/hydrolase, HTG, mixed-linkage glucan:xyloglucan endotransglucosylase, MLG:glucan endotransglucosylase, MLG:xyloglucan endotransglucosylase, More, MXE, XTH9
ECTree
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Substrates Products
Substrates Products on EC 2.4.1.B52 - mixed-linkage glucan endotransglucosylase
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REACTION DIAGRAM
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the grass-specific endotransglucosylase BdXTH8 preferentially uses MLG as a substrate, but also xyloglucan. BdXTH8 does not yield hydrolysis products separable by thin-layer chromatography when acting on xyloglucan or MLG. Mixed-linkage glucan (MLG) consists of several adjacent (1,4)-linked Glc residues separated by a single (1,3)-glucosyl linkage that occurs every third or fourth residue
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additional information
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MLG:xyloglucan endotransglucosylase, MXE, catalyses a reaction similar to XET activity, EC 2.4.1.207, which catalyses the endocleavage of xyloglucan as donor substrate and the creation of a glycosidic link between the newly formed potentially reducing terminus and the nonreducing terminus of another xyloglucan or xyloglucan oligosaccharide molecule as acceptor substrate, but uses mixed-linkage (1->3), (1->4)-beta-D-glucan, MLG,s as a donor substrate as opposed to xyloglucan, cleavage specificity, overview
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additional information
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MLG:xyloglucan endotransglucosylase, MXE, catalyses a reaction similar to XET activity, EC 2.4.1.207, which catalyses the endocleavage of xyloglucan as donor substrate and the creation of a glycosidic link between the newly formed potentially reducing terminus and the nonreducing terminus of another xyloglucan or xyloglucan oligosaccharide molecule as acceptor substrate, but uses mixed-linkage (1->3), (1->4)-beta-D-glucan, MLG,s as a donor substrate as opposed to xyloglucan, cleavage specificity, overview
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additional information
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enzyme predominantly catalyzes hetero-transglycosylation. Its preferred donor substrates (cellulose or mixed-linkage beta-glucan) differ qualitatively from its acceptor substrate xyloglucan. HTG generates stable cellulose-xyloglucan and mixed-linkage beta-glucan-xyloglucan covalent bonds. No donor substrate: cellohexaose
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additional information
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attack of the site of hetero-trans-beta-glucanase (HTG) on one of its donor substrates, mixed-linkage (1->3),(1->4)-beta-D-glucan (MLG, from Equisetum arvense or Hordeum vulgare) is investigated with radioactive oligosaccharides of xyloglucan as the acceptor substrate. Analysis of the structure of the mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) reaction product formed with [3H]XXXGol as the acceptor substrate and barley MLG as the donor substrate via digestion of the product with lichenase, method, overview. MXE forms a (Glc2)-XXXGol disaccharide product, which further analysis reveals as GGXXXGol, where GG is cellobiose. The bond between the GG and the XXXGol moiety is also likely to be a beta-(1->4)-bond because it can be cleaved by cellobiohydrolase. When the tetradecasaccharide [3H]XXXGXXXGol is used as an acceptor substrate, the single radioactive product is GG[3H]XXXGXXXGol. MXE has attached MLG to the non-reducing terminal heptasaccharide subunit alone. Identification of the bond initially cleaved by MXE activity
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additional information
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enzyme FvXTH9 shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE), cellulose:xyloglucan endotransglucosylase (CXE), and xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activities
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