2.4.1.B52: mixed-linkage glucan endotransglucosylase
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For detailed information about mixed-linkage glucan endotransglucosylase, go to the full flat file.
Reaction
transfers a (1->3),(1->4) mixed-linkage glucan to a xyloglucan =
Synonyms
BdTHX8, cellulose:xyloglucan endotransglucosylase, CXE, FvXTH9, grass-specific glycoside hydrolase family 16 endotransglucosylase/hydrolase, HTG, mixed-linkage glucan:xyloglucan endotransglucosylase, MLG:glucan endotransglucosylase, MLG:xyloglucan endotransglucosylase, More, MXE, XTH9
ECTree
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Source Tissue
Source Tissue on EC 2.4.1.B52 - mixed-linkage glucan endotransglucosylase
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FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages
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MXE and XET, EC 2.4.1.207, actions are both detectable in living Equisetum fluviatile shoots, the MXE:XET ratio increasing with age
additional information
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highest in brown, over-wintered leaves compared to young and mature leaves
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transcriptional profiling, BdXTH8, along with the other three genes in the grass-specific clade, is expressed in tissues that accumulate high amounts of MLG. BdXTH8 is highly coexpressed with the transcription factor BdTHX2 in addition to BdTHX1. BdXTH8 and BdTHX2 both have higher expression in elongating internodes and elongating leaf tissue than that in endosperm, while BdTHX1 shows expression in elongating internodes and endosperm tissues
additional information
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no activity in Equisetum arvense strobili and total extracts of these organs, vegetative lateral shoot extracts of Equisetum arvense contain high MXE activity
additional information
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to confirm the transcriptome data, the expression pattern of FvXTH9 is examined by quantitative real-time PCR in fruit at different ripening stages as well as in leaf and flower tissues. FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages. A high expression level of FvXTH9 is also determined in flowers, whereas its mRNA abundance is very low in all other tissues investigated. The expression pattern of FvXTH6 during fruit development resembled that of FvXTH9. FvXTH9 shows the highest expression level in green receptacles