2.4.1.B34: 4,6-alpha-glucanotransferase

This is an abbreviated version!
For detailed information about 4,6-alpha-glucanotransferase, go to the full flat file.

Reaction

The enzyme uses maltooligosaccharides as donor and acceptor substrates. It cleaves alpha1->4 glucosidic bonds and synthesizes 1->6 and 1->4 glucosidic linkages. =

Synonyms

4, 6-alpha-glucanotransferase, 4,6-alpha-GT, 4,6-alpha-GTase, Achr_35950, Exig_2648, GflML4, Gtf106b, GtfB, GTFB protein, GTFB-like 4,6-alpha-glucanotransferase, GtfB-like 4,6-alpha-glucanotransferases, GTFB-like 4,6-alpha-GT, GTFC, GTFC-like 4,6-alpha-glucanotransferase, GTFC-like 4,6-alpha-GT, GtfD, GtfML4, GtfW, GtfX, GtfY, Lreu_1346, UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase stabilizing protein

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.B34 4,6-alpha-glucanotransferase

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Results	in table
1	Activating Compound
6	AA Sequence
2	Application
17	Cloned(Commentary)
1	Crystallization (Commentary)
43	General Information
6	Inhibitors
1	kcat/KM [mM/s]
2	KM Value [mM]
6	Localization
9	Metals/Ions
4	Molecular Weight [Da]
72	Organism
14	pH Optimum
4	pH Range
16	Protein Variants
5	Purification (Commentary)
1	Reaction
40	Reference
6	Source Tissue
5	Specific Activity [micromol/min/mg]
126	Substrates and Products (Substrate)
13	Subunits
101	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
4	Temperature Range [°C]
4	Temperature Stability [°C]
3	Turnover Number [1/s]

Cloned

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Cloned on EC 2.4.1.B34 - 4,6-alpha-glucanotransferase

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expression in Escherichia coli

2 entries

expression in Esdcherichia coli

Limosilactobacillus reuteri

Q5SBM0

721349

expression of N-terminally truncated variant, in Escherichia coli

Limosilactobacillus reuteri

A5VL73, Q5SBN1

735534

gene gftB, recombinant expression in Solanum tuberosum amylose-containing line Kardal and amylose-free mutant amf, the gene is cloned in frame with GBSSI transit peptide to allow amyloplast targeting and is driven by the potato GBSSI promoter for tuber expression, quantitative RT-PCR expression analysis

Limosilactobacillus reuteri

A0A0U5F702

760020

gene gtfB, genomic mapping and phylogenetic analysis, recombinant expression of wild-type and truncated mutant enzymes

Limosilactobacillus reuteri

A0A0U5F702

760207

gene gtfB, recombinant expression of C-terminally His-tagged enzyme mutant GtfB-DELTAN-DELTAV in Escherichia coli strain BL21(DE3)

Limosilactobacillus reuteri

A0A0U5F702

759390

gene gtfB, recombinant expression of His-tagged truncated GtfB mutant in Escherichia coli strain BL21-CodonPlus-(DE3)

Streptococcus thermophilus

759386

gene gtfD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His6-tagged full-length GtfD enzyme without its putative signal peptide-encoding sequence (amino acids 31 to 776) in Escherichia coli strain BL21 Star (DE3)

Paenibacillus beijingensis

760023

gene gtfX, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Ligilactobacillus aviarius

759213

gene gtfY, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Ligilactobacillus aviarius

759213

GTFB-like 4,6-alpha-glucanotransferases, phylogenetic analysis, detailed overview

3 entries

GTFC-like 4,6-alpha-glucanotransferases, sequence comparisons and phylogenetic analysis, detailed overview. A Tyr residue in GTFC-like 4,6-alpha-GTs proteins replaces the subsite +1/+2 Trp residue conserved in almost all GH70 GSs (W1065 in GTF180-DELTAN)

3 entries