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2.4.1.91: flavonol 3-O-glucosyltransferase

This is an abbreviated version!
For detailed information about flavonol 3-O-glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.91

Reaction

UDP-glucose
+
a flavonol
=
UDP
+
a flavonol 3-O-beta-D-glucoside

Synonyms

2,4,5-trichlorophenol detoxifying O-glucosyltransferase, 3GlcT, 3GT, CsUGT78A1, DicGT1, F3GT, FaGT6, FaGT7, flavonoid 3-O-glucosyltransferase, flavonoid 3-O-glycosyltransferase, flavonoid-3-O-glucosyltransferase, flavonol 3-O-glucosyltransferase, flavonol 3-O-GT, glucosyltransferase, uridine diphosphoglucose-flavonol 3-O-, GT-I, GT1, GTI, More, RUGT-10, UDP flavonoid/triterpene GT, UDP glucose-flavonoid 3-O-glucosyltransferase, UDP glucose: flavonoid-3-O-glucosyltransferase, UDP glucose:flavonoid 3-O-glucosyltransferase, UDP-glucose: flavonoid 3-O-glucosyltransferase, UDP-glucose: flavonoid 3-O-glucosyltransferase gene, UDP-glucose:flavonoid 3-O-glucosyltransferase, UDP-glucose:flavonoid 3-O-glycosyltransferase, UDP-glucose:flavonol 3-O-glucosyltransferase, UDPG:flavonoid-3-O-glucosyltransferase, UDPglucose:flavonoid 3-O-glycosyltransferase, UDPglucose:flavonol O3-D-glucosyltransferase, UF3GT, UFGT, UFGT3, UGT706C1, UGT707A3, UGT71G1, UGT78K1, uridine 5'-diphosphate-glucose:flavonoid 3-O-glucosyltransferase, uridine diphosphat-glucose:flavonoid 3-O-glycosyltransferase, VL3GT

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.91 flavonol 3-O-glucosyltransferase

Purification

Purification on EC 2.4.1.91 - flavonol 3-O-glucosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity-purified on immobilized reduced glutathione resin
-
glutathione Sepharose column chromatography
isoenzyme F3GT1 and F3GT2
-
native enzyme from fruits, partially
-
partial
recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli to homogeneity, the His-tag is cleaved off
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration
Talon metal affinity column chromatography
TALON metal affinity resin column chromatography, gel filtration