2.4.1.9: inulosucrase
This is an abbreviated version!
For detailed information about inulosucrase, go to the full flat file.
Word Map on EC 2.4.1.9
-
2.4.1.9
-
farnesyltransferase
-
farnesylation
-
prenylation
-
geranylgeranylation
-
isoprenoids
-
fructooligosaccharides
-
ggtase-i
-
prenyltransferases
-
peptidomimetic
-
h-ras
-
inulin
-
transfructosylation
-
levan
-
ca1a2x
-
15-carbon
-
tipifarnib
-
levansucrase
-
rhob
-
p21ras
-
geranylgeranyltransferase-i
-
reuteri
-
ggtis
-
inulin-type
-
fructosyltransferases
-
1-kestose
-
cvim
-
fructansucrases
-
biotechnology
-
citreum
-
lonafarnib
- 2.4.1.9
-
farnesyltransferase
-
farnesylation
-
prenylation
-
geranylgeranylation
-
isoprenoids
- fructooligosaccharides
- ggtase-i
- prenyltransferases
-
peptidomimetic
- h-ras
- inulin
-
transfructosylation
- levan
-
ca1a2x
-
15-carbon
- tipifarnib
- levansucrase
- rhob
-
p21ras
-
geranylgeranyltransferase-i
- reuteri
-
ggtis
-
inulin-type
- fructosyltransferases
- 1-kestose
- cvim
- fructansucrases
- biotechnology
- citreum
- lonafarnib
Reaction
Synonyms
Ffase, fructansucrase, fructosyltransferase, fructosyltransferase, sucrose 1-, FTase, FTF, GH68 fructansucrase, HugO, INU, InuJ, IS, ISase, IslA, More, sucrose 1-fructosyltransferase, sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase, sucrose:2,1-beta-D-fructan1-beta-D-fructosyltransferase
ECTree
2.4.1.9 inulosucraseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.4.1.9 - inulosucrase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose + sucrose
alpha-D-glucose + medium chain fructooligosaccharides (DP 2-20)
with 300 g/l sucrose, 128.4 g/l of fructooligosaccharide is produced with 85.6% conversion yield. From 400 g/l sucrose, 152.6 g/l of fructooligosaccharides is produced with 76.3% conversion yield
-
-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
3 sucrose + sucrose
3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
3 sucrose + sucrose
3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(21) linkages]
-
-
?
3 sucrose + sucrose
3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(21) linkages]
-
-
?
raffinose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
raffinose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin, determination of inulin product chain length, overview
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin, determination of inulin product chain length, overview
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity. Transglycosylation activity is higher in the whole concentration range tested and completely dominating at higher sucrose concentrations
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
hydrolytic activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
hydrolytic activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
hydrolytic activity
-
-
?
sucrose + H2O
D-fructose + D-glucose
at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose
-
-
?
sucrose + H2O
D-fructose + D-glucose
at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose
-
-
?
sucrose + raffinose
?
synthesizes fructosylraffinose (most likely GalGF2) and a range of larger oligomers (up to GalGF6), and some polymeric material
-
-
?
sucrose + raffinose
?
synthesizes fructosylraffinose (most likely GalGF2) and a range of larger oligomers (up to GalGF6), and some polymeric material
-
-
?
sucrose + raffinose
glucose + 1-kestose
-
-
i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
-
?
sucrose + raffinose
glucose + 1-kestose
-
-
i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
-
?
sucrose + sucrose
1-kestose + nystose + fructooligosaccharides
-
-
-
-
?
sucrose + sucrose
1-kestose + nystose + fructooligosaccharides
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin nanoparticles
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin nanoparticles
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
Limosilactobacillus reuteri TMW1.106
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan polymer
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan polymer
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructooligosaccharides
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructooligosaccharides
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
Limosilactobacillus reuteri TMW1.106
-
-
-
?
sucrose + sucrose
kestose + nystose + fructooligosaccharides
at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose. From the very early stage of the reaction, after 5 min incubation, traces of nystose are visible. The nystose concentration remains constant once it reached a similar level to kestose (after 1 h), and synthesis of FOS of a larger size starts. The enzyme synthesizes mainly a broad range of fructooligosaccharides of the inulin type in a non-processive reaction
-
-
?
sucrose + sucrose
kestose + nystose + fructooligosaccharides
at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose. From the very early stage of the reaction, after 5 min incubation, traces of nystose are visible. The nystose concentration remains constant once it reached a similar level to kestose (after 1 h), and synthesis of FOS of a larger size starts. The enzyme synthesizes mainly a broad range of fructooligosaccharides of the inulin type in a non-processive reaction
-
-
?
sucrose + sucrose
[(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose
-
transgylcosylation activity. Transglycosylation activity is higher in the whole concentration range tested and completely dominating at higher sucrose concentrations
-
-
?
sucrose + sucrose
[(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose
transglycosylation activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C
-
-
?
sucrose + sucrose
[(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose
transglycosylation activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C
-
-
?
sucrose + sucrose
[(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose
-
-
the enzyme selectively produces GF5 from sucose
?
sucrose + sucrose
[(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose
-
-
the enzyme selectively produces GF4 from sucose
?
sucrose + sucrose
[(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose
-
-
the enzyme selectively produces GF4 from sucose
?
sucrose + sucrose
[(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose
-
-
the enzyme selectively produces GF5 from sucose
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
Aspergillus sydowii IAM 2544
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
Bacillus sp. 217C-11
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
Limosilactobacillus reuteri TMW1.106
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
Streptococcus mutans JC-1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
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-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
Weissella confusa MBFCNC-2(1)
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-
?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
via first transfructosylation product 1-kestose, binding mode, overview, an inulin-type linkage is formed. Particular residues from the nonconserved 1B-1C loop determine product linkage type specificity in GH68 fructansucrases
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?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
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?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
via first transfructosylation product 1-kestose, binding mode, overview, an inulin-type linkage is formed. Particular residues from the nonconserved 1B-1C loop determine product linkage type specificity in GH68 fructansucrases
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?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
via first transfructosylation product 1-kestose
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?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
via first transfructosylation product 1-kestose
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-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
-
-
-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
fructoologosaccharides are synthesized from sucrose at the expense of fructose residues from inulin and related polysaccharides. Fructose residues are transferred from polysaccharide to sucrose molecules, forming a trisaccharide in the first instance. Further fructose residues might then be transferred to the trisaccharide, yielding a tetrasaccharide, and so on. No significant reaction with sucrose alone
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?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
-
-
-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
wild-type and recombinant enzyme produce inulin from sucose
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?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
-
-
-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
the recombinant truncated enzyme produces both the fructooligosaccharides 1-kestose and nystose and high-molecular-weight-fructan with beta-(2, 1) linkages. The wild-type enzyme only produces 1-kestose, 95%, and nystose, 5%
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-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
-
-
-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
the enzyme selectively produces GF5 from sucose
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-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
-
-
-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
the enzyme selectively produces GF5 from sucose
-
-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
-
-
-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
the enzyme selectively produces GF5 from sucose
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-
?
additional information
?
-
-
the production of fructooligosaccharide by this enzyme is possible only in the presence of sucrose which is an exclusive donor of fructosyl residue in the transferase reaction. The acceptor can be another molecule of sucrose or frucotoligosaccharide
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?
additional information
?
-
enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview
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-
?
additional information
?
-
-
enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview
-
-
?
additional information
?
-
-
sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside
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-
?
additional information
?
-
-
sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside
-
-
?
additional information
?
-
enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview
-
-
?
additional information
?
-
-
enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview
-
-
?
additional information
?
-
-
very low activity in acceptor reactions with non-sugar acceptors, specificity analysis, overview
-
-
?
additional information
?
-
-
the truncated enzyme mutant IslA4 can synthesize high molecular weight inulin from sucrose, with a residual sucrose hydrolytic activity, and a product specificity similar to the multidomain wild-type enzyme. High sucrose concentrations shift the specificity of the reaction towards fructooligosaccharides (FOS) synthesis, which almost eliminates inulin synthesis and leads to a considerable reduction in sucrose hydrolysis. Reactions with low IslA4 activity and a high sucrose activity allow for high levels of FOS synthesis, where 70% sucrose is used for transfer reactions, with 65% corresponding to transfructosylation for the synthesis of FOS, quantitative product analysis, overview
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-
?
additional information
?
-
the enzyme is able to catalyse a disproportionation type of reaction with 1-kestose, 1,1-nystose and 1,1,1-kestopentaose
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-
?
additional information
?
-
-
the enzyme is able to catalyse a disproportionation type of reaction with 1-kestose, 1,1-nystose and 1,1,1-kestopentaose
-
-
?
additional information
?
-
wild-type inulosucrase synthesizes mostly fructooligosaccharides up to a degree of polymerization of 15 and relatively low amounts of inulin polymer
-
-
?
additional information
?
-
-
the enzyme also hydrolyzes sucrose to D-fructose and D-glucose
-
-
-
additional information
?
-
-
the enzyme also hydrolyzes sucrose to D-fructose and D-glucose
-
-
-
additional information
?
-
wild-type inulosucrase synthesizes mostly fructooligosaccharides up to a degree of polymerization of 15 and relatively low amounts of inulin polymer
-
-
?
additional information
?
-
the enzyme is able to catalyse a disproportionation type of reaction with 1-kestose, 1,1-nystose and 1,1,1-kestopentaose
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-
?
