2.4.1.85: cyanohydrin beta-glucosyltransferase

This is an abbreviated version!
For detailed information about cyanohydrin beta-glucosyltransferase, go to the full flat file.

Reaction

Synonyms

cyanohydrin glucosyltransferase, cyanohydrin glycosyltransferase, glucosyltransferase, uridine diphosphoglucose-p-hydroxymandelonitrile, mandelonitrile glucosyltransferase, sbHMNGT, UDP-glucose-p-hydroxymandelonitrile glucosyltransferase, UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase, UDP-glucosyltransferase, UGT85B1, uridine diphosphoglucose-cyanohydrin glucosyltransferase, uridine diphosphoglucose-p-hydroxymandelonitrile glucosyltransferase, uridine diphosphoglucose:aldehyde cyanohydrin beta-glucosyltransferase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.85 cyanohydrin beta-glucosyltransferase

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Results	in table
3	Activating Compound
43	AA Sequence
3	Application
1	CAS Registry Number
7	Cloned(Commentary)
6	General Information
2	General Stability
6	Inhibitors
12	KM Value [mM]
8	Localization
1	Metals/Ions
1	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
12	Organism
4	Pathway
2	PDB ID
2	pH Optimum
6	Protein Variants
3	Purification (Commentary)
1	Reaction
1	Reaction Type
12	Reference
16	Source Tissue
2	Specific Activity [micromol/min/mg]
2	Storage Stability
46	Substrates and Products (Substrate)
2	Subunits
17	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
10	Turnover Number [1/s]

General Information

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General Information on EC 2.4.1.85 - cyanohydrin beta-glucosyltransferase

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

malfunction

Sorghum bicolor

Q9SBL1

plant tcd2 mutants deficient in the enzyme have reduced vigor, being dwarfed, with poor root development and low fertility. The mutant plants accumulate numerous dhurrin pathway-derived metabolites, some of which are similar to those observed in transgenic Arabidopsis thaliana expressing the CYP79A1 and CYP71E1 genes. The tcd2 mutant suffers from self-intoxication because Sorghum does not have a feedback mechanism to inhibit the initial steps of dhurrin biosynthesis when the glucosyltransferase activity required to complete the synthesis of dhurrin is lacking. Phenotype, detailed overview

736976

metabolism

4 entries

physiological function

Sorghum bicolor

Q9SBL1

enzyme UGT85B1 is essential for formation of dhurrin in sorghum with no co-expressed endogenous UDP-glucosyltransferases able to replace it. Presence of metabolites in the tcd2 mutant which are suggested to be derived from dhurrin via endogenous pathways for nitrogen recovery, indicating which enzymes may be involved in such pathways, metabolites identification by LC-MS

736976

metabolism

Prunus dulcis

B2XBQ5

glucosylation of R-mandelonitrile to prunasin which is a precursor of amygdalin

703819

metabolism

Sorghum bicolor

-

UGT85B1 is an important step in product channelling because a rapid glucosylation of the labile p-hydroxymandelonitrile product of CYP71E1 is necessary to avoid its dissociation into p-hydroxybenzaldehyde and hydrogen cyanide

723366

metabolism

Sorghum bicolor

Q9SBL1

dhurrin is a cyanogenic glucoside and its synthesis from the amino acid tyrosine is catalysed by two membrane-bound cytochrome P450 enzymes (CYP79A1 and CYP71E1) and a soluble glucosyltransferase (UGT85B1), and is dependent on electron transfer from a P450 oxidoreductase

736574

metabolism

Sorghum bicolor

Q9SBL1

dhurrin is a cyanogenic glucoside and its synthesis from the amino acid tyrosine is catalysed by two membrane-bound cytochrome P450 enzymes (CYP79A1 and CYP71E1) and a soluble glucosyltransferase (UGT85B1), and is dependent on electron transfer from a P450 oxidoreductase. Dhurrin biosynthesis in Sorghum and the formation of products derived from the different intermediates as part of the detoxification processes or as putative turnover products identified in the tcd2 mutant, overview

736976