2.4.1.85: cyanohydrin beta-glucosyltransferase

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For detailed information about cyanohydrin beta-glucosyltransferase, go to the full flat file.

Reaction

Synonyms

cyanohydrin glucosyltransferase, cyanohydrin glycosyltransferase, glucosyltransferase, uridine diphosphoglucose-p-hydroxymandelonitrile, mandelonitrile glucosyltransferase, sbHMNGT, UDP-glucose-p-hydroxymandelonitrile glucosyltransferase, UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase, UDP-glucosyltransferase, UGT85B1, uridine diphosphoglucose-cyanohydrin glucosyltransferase, uridine diphosphoglucose-p-hydroxymandelonitrile glucosyltransferase, uridine diphosphoglucose:aldehyde cyanohydrin beta-glucosyltransferase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.85 cyanohydrin beta-glucosyltransferase

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Results	in table
3	Activating Compound
43	AA Sequence
3	Application
1	CAS Registry Number
7	Cloned(Commentary)
6	General Information
2	General Stability
6	Inhibitors
12	KM Value [mM]
8	Localization
1	Metals/Ions
1	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
12	Organism
4	Pathway
2	PDB ID
2	pH Optimum
6	Protein Variants
3	Purification (Commentary)
1	Reaction
1	Reaction Type
12	Reference
16	Source Tissue
2	Specific Activity [micromol/min/mg]
2	Storage Stability
46	Substrates and Products (Substrate)
2	Subunits
17	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
10	Turnover Number [1/s]

Cloned

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Cloned on EC 2.4.1.85 - cyanohydrin beta-glucosyltransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cloning of a full-length cDNA encoding enzyme, expression in Escherichia coli JM109, 492-amino acids translation product

Sorghum bicolor

Q9SBL1

489461

expressed in Arabidopsis thaliana

Sorghum bicolor

Q9SBL1

694605

expressed in Escherichia coli

Prunus dulcis

B2XBQ5

703819

gene UGT85B1, DNA and amino acid sequence determination and analysis of wild-type and mutant tcd2 genes. The CYP79A1, CYP71E1 and UGT85B1 genes required for dhurrin synthesis are clustered

Sorghum bicolor

Q9SBL1

736976

mutant enzymes, expression in Escherichia coli

Sorghum bicolor

Q9SBL1

660260

recombinant expression in Nicotiana tabacum chloroplasts, coexpression with CYP79A1 and CYP71E1 linked in a tricistronic operon construct with the essential regulatory elements

Sorghum bicolor

Q9SBL1

736574

transgenic Arabidopsis thaliana plants expressing the entire biosynthetic pathway for the tyrosine-derived cyanogenic glucoside dhurrin as accomplished by insertion of CYP79A1, CYP71E2, and UFT85B1 are shown to accumulate 4% dry-weight dhurrin with marginal inadvertent effects on plant morphology, free amino acid pools, transcriptome, and metabolome

Sorghum bicolor

-

660383