Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.1.80: ceramide glucosyltransferase

This is an abbreviated version!
For detailed information about ceramide glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.80

Reaction

UDP-alpha-D-glucose
+
an N-acylsphingosine
=
UDP
+
a beta-D-glucosyl-N-acylsphingosine

Synonyms

ceramide glucosyltransferase, ceramide glycosyltransferase, ceramide:UDP-glucose glucosyltransferase, ceramide:UDPGlc glucosyltransferase, cerebroside synthase, CGT, cgt-1, cgt-2, cgt-3, GCS, GCS1, GlcCer synthase, GlcCerS, GlcT, GlcT-1, glucosylceramide synthase, glucosyltransferase, uridine diphosphoglucose-ceramide, GluT, UDP glucose-ceramide glucosyltransferase, UDP-Glc:ceramide glucosyltransferase, UDP-glucoase:ceramide glucosyltransferase, UDP-glucose ceramide glucosyltransferase, UDP-glucose ceramide glycosyltransferase, UDP-glucose: ceramide glucosyltransferase, UDP-glucose:ceramide glucosyltransferase, Ugcg, uridine diphosphate (UDP)-glucose:N-acylsphingosine D-glucosyltransferase, uridine diphosphoglucose-ceramide glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.80 ceramide glucosyltransferase

Engineering

Engineering on EC 2.4.1.80 - ceramide glucosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTAFgGCS1
-
a null mutation of the FgGCS1 gene becomes resistant to antifungal defensins MsDef1, but not to MtDef4 from Medicago. It shows a significant change in the conidial morphology and displays dramatic polar growth defect, and its mycelia are resistant to cell wall degrading enzymes.GCS1 is not required for pathogenicity of Fusarium graminearum
C143A
site-directed mutagenesis, increased activity, reduced expression level compared to wild-type
C207A
site-directed mutagenesis, reduced activity, reduced inhibitory effect of N-ethylmaleimide, reduced expression level compared to wild-type
C213A
site-directed mutagenesis, reduced activity, reduced expression level compared to wild-type
C232A
site-directed mutagenesis, reduced expresssion level compared to wild-type
C290A
site-directed mutagenesis, reduced expression level compared to wild-type
C296A
site-directed mutagenesis, reduced activity, reduced expression level compared to wild-type
C321A
site-directed mutagenesis, reduced activity
C321A/C323A
site-directed mutagenesis, reduced activity
C343A
site-directed mutagenesis, enhanced activity, slightly reduced expression level compared to wild-type
C384A
site-directed mutagenesis, enhanced activity
C86A
site-directed mutagenesis, reduced activity, reduced expression level compared to wild-type
C98A
site-directed mutagenesis, reduced activity, reduced expression level compared to wild-type
H169A
26.4% activity compared to wild-type, reduced inhibition by diethyldicarbonate and increased protection by UDP-glucose
H193A
33.0% activity compared to wild-type, reduced inhibition by diethyldicarbonate and reduced protection by UDP-glucose
H193D
no activity
H193N
23.0% activity compared to wild-type, reduced inhibition by diethyldicarbonate and reduced protection by UDP-glucose
H193R
no activity
H26A
5.2% activity compared to wild-type
H26D
10.5% activity compared to wild-type
H26N
44.5% activity compared to wild-type
H26R
118.5% activity compared to wild-type, slightly enhanced inhibition by diethyldicarbonate and slightly reduced protection by UDP-glucose
H308A
35.2% activity compared to wild-type, slightly reduced inhibition by diethyldicarbonate and slightly reduced protection by UDP-glucose
H308A/H309A
10.8% activity compared to wild-type
H309A
69.1% activity compared to wild-type
H322A
3.8% activity compared to wild-type
H322D
2.6% activity compared to wild-type
H322N
49.8% activity compared to wild-type, slightly enhanced inhibition by diethyldicarbonate and slightly reduced protection by UDP-glucose
H322R
no activity
H36A
103.2% activity compared to wild-type, slightly enhanced inhibition by diethyldicarbonate and slightly reduced protection by UDP-glucose
H90A
119.3% activity compared to wild-type
additional information