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2.4.1.7: sucrose phosphorylase

This is an abbreviated version!
For detailed information about sucrose phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.7

Reaction

sucrose
+
phosphate
=
D-fructose
+
alpha-D-glucose 1-phosphate

Synonyms

1149SPase, 1355SPase, 742SPase, BiSP, disaccharide glucosyltransferase, LmSPase, More, SPase, sucrose glucosyltransferase, sucrose: orthophosphate, alpha-D-glucosyltransferase, sucrose: phosphate alpha-D-glucosyltransferase, unspase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.7 sucrose phosphorylase

Reference

Reference on EC 2.4.1.7 - sucrose phosphorylase

Please use the Reference Search for a specific query.
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Doudoroff, M.
Disaccharide phosphorylases
The Enzymes, 2nd. Ed. (Boyer, P. D. , Lardy, H. , Myrbaeck, K. , eds. )
5
229-236
1961
Leuconostoc mesenteroides, Shewanella putrefaciens, Pelomonas saccharophila
-
Manually annotated by BRENDA team
Silverstein, R.; Voet, J.; Reed, D.; Abeles, R.H.
Purification and mechanism of action of sucrose phosphorylase
J. Biol. Chem.
242
1338-1346
1967
Pelomonas saccharophila
Manually annotated by BRENDA team
Mieyal, J.J.; Simon, M.; Abeles, R.H.
Mechanism of action of sucrose phosphorylase. III. The reaction with water and other alcohols
J. Biol. Chem.
247
532-542
1972
Pelomonas saccharophila
Manually annotated by BRENDA team
Taylor, F.; Chen, L.; Gong, C.S.; Tsao, G.T.
Kinetics of immobilized sucrose phosphorylase
Biotechnol. Bioeng.
24
317-328
1982
Pelomonas saccharophila
Manually annotated by BRENDA team
Koga, T.; Nakamura, K.; Shirokane, Y.; Mizusawa, K.; Kitao, S.; Kikuchi, M.
Purification and some properties of sucrose phosphorylase from Leuconostoc mesenteroides
Agric. Biol. Chem.
55
1805-1810
1991
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Kitao, S.; Sekine, H.
Transglucosylation catalyzed by sucrose phosphorylase from Leuconostoc mesenteroides and production of glycosyl-xylitol
Biosci. Biotechnol. Biochem.
56
2011-2014
1992
Leuconostoc mesenteroides
-
Manually annotated by BRENDA team
Kitao, S.; Nakano, E.
Cloning of the sucrose phosphorylase gene from Leuconostoc mesenteroides and its overexpression using a 'sleeper' bacteriophage vector
J. Ferment. Bioeng.
73
179-184
1992
Leuconostoc mesenteroides
-
Manually annotated by BRENDA team
Kitao, S.; Ariga, T.; Matsudo, T.; Sekine, H.
The syntheses of catechin-glucosides by transglycosylation with Leuconostoc mesenteroides sucrose phosphorylase
Biosci. Biotechnol. Biochem.
57
2010-2015
1993
Leuconostoc mesenteroides
-
Manually annotated by BRENDA team
Kitao, S.; Sekine, H.
Syntheses of two kojic acid glucosides with sucrose phosphorylase from Leuconostoc mesenteroides
Biosci. Biotechnol. Biochem.
58
419-420
1994
Leuconostoc mesenteroides
-
Manually annotated by BRENDA team
Ichikawa, M.; Schnaar, R.L.; Ichikawa, Y.
Application of sucrose phosphorylase reaction in one-pot enzymic galactosylation: scavenger of phosphate and generation of glucose 1-phosphate in situ
Tetrahedron Lett.
36
8731-8732
1995
Pelomonas saccharophila
-
Manually annotated by BRENDA team
Kawasaki, H.; Nakamura, N.; Ohmori, M.; Amari, K.; Sakai, T.
Screening for bacteria producing sucrose phosphorylase and characterization of the enzymes
Biosci. Biotechnol. Biochem.
60
319-321
1996
Leuconostoc mesenteroides, Leuconostoc mesenteroides AKU 1102
Manually annotated by BRENDA team
Tonouchi, N.; Horinouchi, S.; Tsuchida, T.; Yoshinaga, F.
Increased cellulose production from sucrose by Acetobacter after introducing the sucrose phosphorylase gene
Biosci. Biotechnol. Biochem.
62
1778-1780
1998
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Kitao, S.; Matsudo, T.; Sasaki, T.; Koga, T.; Kawamura, M.
Enzymatic synthesis of stable, odorless, and powdered furanone glucosides by sucrose phosphorylase
Biosci. Biotechnol. Biochem.
64
134-141
2000
Leuconostoc mesenteroides
Manually annotated by BRENDA team
van den Broek, L.A.; van Boxtel, E.L.; Kievit, R.P.; Verhoef, R.; Beldman, G.; Voragen, A.G.
Physico-chemical and transglucosylation properties of recombinant sucrose phosphorylase from Bifidobacterium adolescentis DSM20083
Appl. Microbiol. Biotechnol.
65
219-227
2004
Bifidobacterium adolescentis (Q84HQ2), Bifidobacterium adolescentis
Manually annotated by BRENDA team
Sprogoe, D.; van den Broek, L.A.; Mirza, O.; Kastrup, J.S.; Voragen, A.G.; Gajhede, M.; Skov, L.K.
Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis
Biochemistry
43
1156-1162
2004
Bifidobacterium adolescentis (Q84HQ2), Bifidobacterium adolescentis
Manually annotated by BRENDA team
Kim, M.; Kwon, T.; Lee, H.J.; Kim, K.H.; Chung, D.K.; Ji, G.E.; Byeon, E.S.; Lee, J.H.
Cloning and expression of sucrose phosphorylase gene from Bifidobacterium longum in E. coli and characterization of the recombinant enzyme
Biotechnol. Lett.
25
1211-1217
2003
Bifidobacterium longum
Manually annotated by BRENDA team
Kwon, T.; Kim, C.T.; Lee, J.H.
Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum
Biotechnol. Lett.
29
611-615
2007
Bifidobacterium longum
Manually annotated by BRENDA team
Lee, J.; Yoon, S.; Nam, S.; Moon, Y.; Moon, Y.; Kim, D.
Molecular cloning of a gene encoding the sucrose phosphorylase from Leuconostoc mesenteroides B-1149 and the expression in Escherichia coli
Enzyme Microb. Technol.
39
612-620
2006
Leuconostoc mesenteroides (Q14EH6), Leuconostoc mesenteroides B-1149 (Q14EH6)
-
Manually annotated by BRENDA team
Schwarz, A.; Nidetzky, B.
Asp-196-->Ala mutant of Leuconostoc mesenteroides sucrose phosphorylase exhibits altered stereochemical course and kinetic mechanism of glucosyl transfer to and from phosphate
FEBS Lett.
580
3905-3910
2006
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Mueller, M.; Nidetzky, B.
The role of Asp-295 in the catalytic mechanism of Leuconostoc mesenteroides sucrose phosphorylase probed with site-directed mutagenesis
FEBS Lett.
581
1403-1408
2007
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Fujii, K.; Iiboshi, M.; Yanase, M.; Takaha, T.; Kuriki, T.
Enhancing the thermal stability of sucrose phosphorylase from Streptococcus mutans by random mutagenesis
J. Appl. Glycosci.
53
91-97
2006
Streptococcus mutans
-
Manually annotated by BRENDA team
Mirza, O.; Skov, L.K.; Sprogoe, D.; van den Broek, L.A.; Beldman, G.; Kastrup, J.S.; Gajhede, M.
Structural rearrangements of sucrose phosphorylase from Bifidobacterium adolescentis during sucrose conversion
J. Biol. Chem.
281
35576-35584
2006
Bifidobacterium adolescentis (Q84HQ2), Bifidobacterium adolescentis
Manually annotated by BRENDA team
Goedl, C.; Schwarz, A.; Minani, A.; Nidetzky, B.
Recombinant sucrose phosphorylase from Leuconostoc mesenteroides: characterization, kinetic studies of transglucosylation, and application of immobilised enzyme for production of alpha-D-glucose 1-phosphate
J. Biotechnol.
129
77-86
2007
Leuconostoc mesenteroides, Leuconostoc mesenteroides (Q59495)
Manually annotated by BRENDA team
Schwarz, A.; Brecker, L.; Nidetzky, B.
Acid-base catalysis in Leuconostoc mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu237-Gln mutant enzymes
Biochem. J.
403
441-449
2007
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Nomura, K.; Sugimoto, K.; Nishiura, H.; Ohdan, K.; Nishimura, T.; Hayashi, H.; Kuriki, T.
Glucosylation of acetic acid by sucrose phosphorylase
Biosci. Biotechnol. Biochem.
72
82-87
2008
Streptococcus mutans
Manually annotated by BRENDA team
Lee, J.H.; Moon, Y.H.; Kim, N.; Kim, Y.M.; Kang, H.K.; Jung, J.Y.; Abada, E.; Kang, S.S.; Kim, D.
Cloning and expression of the sucrose phosphorylase gene from Leuconostoc mesenteroides in Escherichia coli
Biotechnol. Lett.
30
749-754
2008
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-742
Manually annotated by BRENDA team
Goedl, C.; Schwarz, A.; Mueller, M.; Brecker, L.; Nidetzky, B.
Mechanistic differences among retaining disaccharide phosphorylases: insights from kinetic analysis of active site mutants of sucrose phosphorylase and alpha,alpha-trehalose phosphorylase
Carbohydr. Res.
343
2032-2040
2008
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Mueller, M.; Nidetzky, B.
Dissecting differential binding of fructose and phosphate as leaving group/nucleophile of glucosyl transfer catalyzed by sucrose phosphorylase
FEBS Lett.
581
3814-3818
2007
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Sugimoto, K.; Nomura, K.; Nishiura, H.; Ohdan, K.; Kamasaki, H.; Nishimura, T.; Hayashi, H.; Kuriki, T.
Novel transglucosylating reaction of sucrose phosphorylase to carboxylic compounds
J. Appl. Glycosci.
55
119-125
2008
Streptococcus mutans
-
Manually annotated by BRENDA team
Sugimoto, K.; Nomura, K.; Nishiura, H.; Ohdan, K.; Ohdan, K.; Hayashi, H.; Kuriki, T.
Novel transglucosylating reaction of sucrose phosphorylase to carboxylic compounds such as benzoic acid
J. Biosci. Bioeng.
104
22-29
2007
Leuconostoc mesenteroides, Streptococcus mutans
Manually annotated by BRENDA team
Shin, M.H.; Jung, M.W.; Lee, J.; Kim, M.D.; Kim, K.H.
Strategies for producing recombinant sucrose phosphorylase originating from Bifidobacterium longum in Escherichia coli JM109
Process Biochem.
43
822-828
2008
Bifidobacterium longum
-
Manually annotated by BRENDA team
Goedl, C.; Sawangwan, T.; Wildberger, P.; Nidetzky, B.
Sucrose phosphorylase: A powerful transglucosylation catalyst for synthesis of alpha-D-glucosides as industrial fine chemicals
Biocatal. Biotransform.
28
10-21
2010
Leuconostoc mesenteroides, Pelomonas saccharophila, Streptococcus mutans, Bifidobacterium adolescentis (Q84HQ2), Leuconostoc mesenteroides B-1149
-
Manually annotated by BRENDA team
Abada, E.; Osman, M.; Lee, J.; Kim, D.
Molecular cloning of the gene 1355SPase encoding a sucrose phosphorylase from the bacterium Leuconostoc mesenteroides B-1355
Biotechnology
7
463-468
2008
Leuconostoc mesenteroides (B0F411), Leuconostoc mesenteroides NRRL B-1355 (B0F411)
-
Manually annotated by BRENDA team
Goedl, C.; Nidetzky, B.
Sucrose phosphorylase harbouring a redesigned, glycosyltransferase-like active site exhibits retaining glucosyl transfer in the absence of a covalent intermediate
ChemBioChem
10
2333-2337
2009
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Shin, M.; Cheong, N.; Lee, J.; Kim, K.
Transglucosylation of caffeic acid by a recombinant sucrose phosphorylase in aqueous buffer and aqueous-supercritical CO2 media
Food Chem.
115
1028-1033
2009
Bifidobacterium longum
-
Manually annotated by BRENDA team
Kasperowicz, A.; Stan-Glasek, K.; Guczynska, W.; Piknova, M.; Pristas, P.; Nigutova, K.; Javorsky, P.; Michalowski, T.
Sucrose phosphorylase of the rumen bacterium Pseudobutyrivibrio ruminis strain A
J. Appl. Microbiol.
107
812-820
2009
Pseudobutyrivibrio ruminis, Pseudobutyrivibrio ruminis A
Manually annotated by BRENDA team
Wildberger, P.; Todea, A.; Nidetzky, B.
Probing enzyme substrate interactions at the catalytic subsite of Leuconostoc mesenteroides sucrose phosphorylase with site-directed mutagenesis: The roles of Asp
Biocatal. Biotransform.
30
326-337
2012
Leuconostoc mesenteroides
-
Manually annotated by BRENDA team
Cerdobbel, A.; De Winter, K.; Desmet, T.; Soetaert, W.
Sucrose phosphorylase as cross-linked enzyme aggregate: improved thermal stability for industrial applications
Biotechnol. J.
5
1192-1197
2010
Bifidobacterium adolescentis
Manually annotated by BRENDA team
Luley-Goedl, C.; Sawangwan, T.; Brecker, L.; Wildberger, P.; Nidetzky, B.
Regioselective O-glucosylation by sucrose phosphorylase: a promising route for functional diversification of a range of 1,2-propanediols
Carbohydr. Res.
345
1736-1740
2010
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Wildberger, P.; Luley-Goedl, C.; Nidetzky, B.
Aromatic interactions at the catalytic subsite of sucrose phosphorylase: their roles in enzymatic glucosyl transfer probed with Phe52->Ala and Phe52->Asn mutants
FEBS Lett.
585
499-504
2011
Leuconostoc mesenteroides
Manually annotated by BRENDA team
De Winter, K.; Soetaert, W.; Desmet, T.
An imprinted cross-linked enzyme aggregate (iCLEA) of sucrose phosphorylase: combining improved stability with altered specificity
Int. J. Mol. Sci.
13
11333-11342
2012
Bifidobacterium adolescentis
Manually annotated by BRENDA team
Wiesbauer, J.; Goedl, C.; Schwarz, A.; Brecker, L.; Nidetzky, B.
Substitution of the catalytic acid-base Glu237 by Gln suppresses hydrolysis during glucosylation of phenolic acceptors catalyzed by Leuconostoc mesenteroides sucrose phosphorylase
J. Mol. Catal. B
65
24-29
2010
Leuconostoc mesenteroides
-
Manually annotated by BRENDA team
Renirie, R.; Pukin, A.; Van Lagen, B.; Franssen, M.
Regio- and stereoselective glucosylation of diols by sucrose phosphorylase using sucrose or glucose 1-phosphate as glucosyl donor
J. Mol. Catal. B
67
219-224
2010
Leuconostoc mesenteroides
-
Manually annotated by BRENDA team
Cerdobbel, A.; De Winter, K.; Aerts, D.; Kuipers, R.; Joosten, H.; Soetaert, W.; Desmet, T.
Increasing the thermostability of sucrose phosphorylase by a combination of sequence- and structure-based mutagenesis
Protein Eng. Des. Sel.
24
829-834
2011
Bifidobacterium adolescentis
Manually annotated by BRENDA team
Kasperowicz, A.; Stan-Glasek, K.; Guczynska, W.; Pristas, P.; Javorsky, P.; Vandzurova, A.; Michalowski, T.
?-Fructofuranosidase and sucrose phosphorylase of rumen bacterium Pseudobutyrivibrio ruminis strain 3
World J. Microbiol. Biotechnol.
28
1271-1279
2012
Pseudobutyrivibrio ruminis, Pseudobutyrivibrio ruminis 3
Manually annotated by BRENDA team
Du, L.; Yang, H.; Huo, Y.; Wei, H.; Xu, Y.; Wei, Y.; Huang, R.
A novel sucrose phosphorylase from the metagenomes of sucrose-rich environment: isolation and characterization
World J. Microbiol. Biotechnol.
28
2871-2878
2012
uncultured bacterium (B8Y3Y0)
Manually annotated by BRENDA team
Aerts, D.; Verhaeghe, T.; Joosten, H.J.; Vriend, G.; Soetaert, W.; Desmet, T.
Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input
Biotechnol. Bioeng.
110
2563-2572
2013
Streptococcus mutans
Manually annotated by BRENDA team
Kraus, M.; Grimm, C.; Seibel, J.
Redesign of the active site of sucrose phosphorylase through a clash-induced cascade of loop shifts
ChemBioChem
17
33-36
2016
Bifidobacterium adolescentis (Q84HQ2), Bifidobacterium adolescentis
Manually annotated by BRENDA team
Rocha, J.M.; Caseli, L.
Adsorption and enzyme activity of sucrose phosphorylase on lipid Langmuir and Langmuir-Blodgett films
Colloids Surf. B Biointerfaces
116
497-501
2014
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Teixeira, J.S.; Abdi, R.; Su, M.S.; Schwab, C.; Gaenzle, M.G.
Functional characterization of sucrose phosphorylase and scrR, a regulator of sucrose metabolism in Lactobacillus reuteri
Food Microbiol.
36
432-439
2013
Limosilactobacillus reuteri (M9ZS93), Limosilactobacillus reuteri, Limosilactobacillus reuteri LTH5448 (M9ZS93)
Manually annotated by BRENDA team
De Winter, K.; Verlinden, K.; Kren, V.; Weignerova¡, L.; Soetaert, W.; Desmet, T.
Ionic liquids as cosolvents for glycosylation by sucrose phosphorylase: balancing acceptor solubility and enzyme stability
Green Chem.
15
1949-1955
2013
Bifidobacterium adolescentis (Q84HQ2)
-
Manually annotated by BRENDA team
Morimoto, K.; Yoshihara, A.; Furumoto, T.; Takata, G.
Production and application of a rare disaccharide using sucrose phosphorylase from Leuconostoc mesenteroides
J. Biosci. Bioeng.
119
652-656
2015
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Verhaeghe, T.; Diricks, M.; Aerts, D.; Soetaert, W.; Desmet, T.
Mapping the acceptor site of sucrose phosphorylase from Bifidobacterium adolescentis by alanine scanning
J. Mol. Catal. B
96
81-88
2013
Bifidobacterium adolescentis (Q84HQ2)
-
Manually annotated by BRENDA team
Renesteen, E.; Cahyo, F.; Malik, A.
Transglycosylation activity and characterization of recombinant sucrose phosphorylase from Leuconostoc mesenteroides MBFWRS-3(1) expressed in Escherichia coli
Int. J. App. Pharm.
12
264-267
2020
Leuconostoc mesenteroides (E2IHA5), Leuconostoc mesenteroides MBFWRS-3(1) (E2IHA5)
-
Manually annotated by BRENDA team
Bolivar, J.M.; Luley-Goedl, C.; Leitner, E.; Sawangwan, T.; Nidetzky, B.
Production of glucosyl glycerol by immobilized sucrose phosphorylase Options for enzyme fixation on a solid support and application in microscale flow format
J. Biotechnol.
257
131-138
2017
Leuconostoc mesenteroides
Manually annotated by BRENDA team
Li, Y.; Li, Z.; He, X.; Chen, L.; Cheng, Y.; Jia, H.; Yan, M.; Chen, K.
Characterisation of a Thermobacillus sucrose phosphorylase and its utility in enzymatic synthesis of 2-O-beta-D-glucopyranosyl-L-ascorbic acid
J. Biotechnol.
305
27-34
2019
Thermobacillus sp. ZCTH02-B1 (A0A1Y3Q6Q6)
Manually annotated by BRENDA team
Wang, M.; Wu, J.; Wu, D.
Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme
Microb. Cell Fact.
17
23
2018
Bifidobacterium adolescentis
Manually annotated by BRENDA team
Yao, D.; Fan, J.; Han, R.; Xiao, J.; Li, Q.; Xu, G.; Dong, J.; Ni, Y.
Enhancing soluble expression of sucrose phosphorylase in Escherichia coli by molecular chaperones
Protein Expr. Purif.
169
105571
2020
Thermoanaerobacterium thermosaccharolyticum (WP_094046414.1), Thermoanaerobacterium thermosaccharolyticum
Manually annotated by BRENDA team
Zhang, H.; Sun, X.; Li, W.; Li, T.; Li, S.; Kitaoka, M.
Expression and characterization of recombinant sucrose phosphorylase
Protein J.
37
93-100
2018
Bifidobacterium longum (A5A8M5), Bifidobacterium longum JCM1217 (A5A8M5)
Manually annotated by BRENDA team