2.4.1.7: sucrose phosphorylase
This is an abbreviated version!
For detailed information about sucrose phosphorylase, go to the full flat file.
Word Map on EC 2.4.1.7
-
2.4.1.7
-
mesenteroides
-
leuconostoc
-
bifidobacterium
-
adolescentis
-
phosphorylases
-
transglucosylation
-
synthesis
-
alpha-d-glucose
-
laminaribiose
-
deglucosylation
-
dextransucrase
-
pseudobutyrivibrio
-
ruminis
-
kojibiose
-
medicine
-
industry
- 2.4.1.7
- mesenteroides
- leuconostoc
-
bifidobacterium
- adolescentis
- phosphorylases
-
transglucosylation
- synthesis
- alpha-d-glucose
- laminaribiose
-
deglucosylation
- dextransucrase
-
pseudobutyrivibrio
- ruminis
- kojibiose
- medicine
- industry
Reaction
Synonyms
1149SPase, 1355SPase, 742SPase, BiSP, disaccharide glucosyltransferase, LmSPase, More, SPase, sucrose glucosyltransferase, sucrose: orthophosphate, alpha-D-glucosyltransferase, sucrose: phosphate alpha-D-glucosyltransferase, unspase
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Reference
Reference on EC 2.4.1.7 - sucrose phosphorylase
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Doudoroff, M.
Disaccharide phosphorylases
The Enzymes, 2nd. Ed. (Boyer, P. D. , Lardy, H. , Myrbaeck, K. , eds. )
5
229-236
1961
Leuconostoc mesenteroides, Shewanella putrefaciens, Pelomonas saccharophila
-
Silverstein, R.; Voet, J.; Reed, D.; Abeles, R.H.
Purification and mechanism of action of sucrose phosphorylase
J. Biol. Chem.
242
1338-1346
1967
Pelomonas saccharophila
Mieyal, J.J.; Simon, M.; Abeles, R.H.
Mechanism of action of sucrose phosphorylase. III. The reaction with water and other alcohols
J. Biol. Chem.
247
532-542
1972
Pelomonas saccharophila
Taylor, F.; Chen, L.; Gong, C.S.; Tsao, G.T.
Kinetics of immobilized sucrose phosphorylase
Biotechnol. Bioeng.
24
317-328
1982
Pelomonas saccharophila
Koga, T.; Nakamura, K.; Shirokane, Y.; Mizusawa, K.; Kitao, S.; Kikuchi, M.
Purification and some properties of sucrose phosphorylase from Leuconostoc mesenteroides
Agric. Biol. Chem.
55
1805-1810
1991
Leuconostoc mesenteroides
Kitao, S.; Sekine, H.
Transglucosylation catalyzed by sucrose phosphorylase from Leuconostoc mesenteroides and production of glycosyl-xylitol
Biosci. Biotechnol. Biochem.
56
2011-2014
1992
Leuconostoc mesenteroides
-
Kitao, S.; Nakano, E.
Cloning of the sucrose phosphorylase gene from Leuconostoc mesenteroides and its overexpression using a 'sleeper' bacteriophage vector
J. Ferment. Bioeng.
73
179-184
1992
Leuconostoc mesenteroides
-
Kitao, S.; Ariga, T.; Matsudo, T.; Sekine, H.
The syntheses of catechin-glucosides by transglycosylation with Leuconostoc mesenteroides sucrose phosphorylase
Biosci. Biotechnol. Biochem.
57
2010-2015
1993
Leuconostoc mesenteroides
-
Kitao, S.; Sekine, H.
Syntheses of two kojic acid glucosides with sucrose phosphorylase from Leuconostoc mesenteroides
Biosci. Biotechnol. Biochem.
58
419-420
1994
Leuconostoc mesenteroides
-
Ichikawa, M.; Schnaar, R.L.; Ichikawa, Y.
Application of sucrose phosphorylase reaction in one-pot enzymic galactosylation: scavenger of phosphate and generation of glucose 1-phosphate in situ
Tetrahedron Lett.
36
8731-8732
1995
Pelomonas saccharophila
-
Kawasaki, H.; Nakamura, N.; Ohmori, M.; Amari, K.; Sakai, T.
Screening for bacteria producing sucrose phosphorylase and characterization of the enzymes
Biosci. Biotechnol. Biochem.
60
319-321
1996
Leuconostoc mesenteroides, Leuconostoc mesenteroides AKU 1102
Tonouchi, N.; Horinouchi, S.; Tsuchida, T.; Yoshinaga, F.
Increased cellulose production from sucrose by Acetobacter after introducing the sucrose phosphorylase gene
Biosci. Biotechnol. Biochem.
62
1778-1780
1998
Leuconostoc mesenteroides
Kitao, S.; Matsudo, T.; Sasaki, T.; Koga, T.; Kawamura, M.
Enzymatic synthesis of stable, odorless, and powdered furanone glucosides by sucrose phosphorylase
Biosci. Biotechnol. Biochem.
64
134-141
2000
Leuconostoc mesenteroides
van den Broek, L.A.; van Boxtel, E.L.; Kievit, R.P.; Verhoef, R.; Beldman, G.; Voragen, A.G.
Physico-chemical and transglucosylation properties of recombinant sucrose phosphorylase from Bifidobacterium adolescentis DSM20083
Appl. Microbiol. Biotechnol.
65
219-227
2004
Bifidobacterium adolescentis (Q84HQ2), Bifidobacterium adolescentis
Sprogoe, D.; van den Broek, L.A.; Mirza, O.; Kastrup, J.S.; Voragen, A.G.; Gajhede, M.; Skov, L.K.
Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis
Biochemistry
43
1156-1162
2004
Bifidobacterium adolescentis (Q84HQ2), Bifidobacterium adolescentis
Kim, M.; Kwon, T.; Lee, H.J.; Kim, K.H.; Chung, D.K.; Ji, G.E.; Byeon, E.S.; Lee, J.H.
Cloning and expression of sucrose phosphorylase gene from Bifidobacterium longum in E. coli and characterization of the recombinant enzyme
Biotechnol. Lett.
25
1211-1217
2003
Bifidobacterium longum
Kwon, T.; Kim, C.T.; Lee, J.H.
Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum
Biotechnol. Lett.
29
611-615
2007
Bifidobacterium longum
Lee, J.; Yoon, S.; Nam, S.; Moon, Y.; Moon, Y.; Kim, D.
Molecular cloning of a gene encoding the sucrose phosphorylase from Leuconostoc mesenteroides B-1149 and the expression in Escherichia coli
Enzyme Microb. Technol.
39
612-620
2006
Leuconostoc mesenteroides (Q14EH6), Leuconostoc mesenteroides B-1149 (Q14EH6)
-
Schwarz, A.; Nidetzky, B.
Asp-196-->Ala mutant of Leuconostoc mesenteroides sucrose phosphorylase exhibits altered stereochemical course and kinetic mechanism of glucosyl transfer to and from phosphate
FEBS Lett.
580
3905-3910
2006
Leuconostoc mesenteroides
Mueller, M.; Nidetzky, B.
The role of Asp-295 in the catalytic mechanism of Leuconostoc mesenteroides sucrose phosphorylase probed with site-directed mutagenesis
FEBS Lett.
581
1403-1408
2007
Leuconostoc mesenteroides
Fujii, K.; Iiboshi, M.; Yanase, M.; Takaha, T.; Kuriki, T.
Enhancing the thermal stability of sucrose phosphorylase from Streptococcus mutans by random mutagenesis
J. Appl. Glycosci.
53
91-97
2006
Streptococcus mutans
-
Mirza, O.; Skov, L.K.; Sprogoe, D.; van den Broek, L.A.; Beldman, G.; Kastrup, J.S.; Gajhede, M.
Structural rearrangements of sucrose phosphorylase from Bifidobacterium adolescentis during sucrose conversion
J. Biol. Chem.
281
35576-35584
2006
Bifidobacterium adolescentis (Q84HQ2), Bifidobacterium adolescentis
Goedl, C.; Schwarz, A.; Minani, A.; Nidetzky, B.
Recombinant sucrose phosphorylase from Leuconostoc mesenteroides: characterization, kinetic studies of transglucosylation, and application of immobilised enzyme for production of alpha-D-glucose 1-phosphate
J. Biotechnol.
129
77-86
2007
Leuconostoc mesenteroides, Leuconostoc mesenteroides (Q59495)
Schwarz, A.; Brecker, L.; Nidetzky, B.
Acid-base catalysis in Leuconostoc mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu237-Gln mutant enzymes
Biochem. J.
403
441-449
2007
Leuconostoc mesenteroides
Nomura, K.; Sugimoto, K.; Nishiura, H.; Ohdan, K.; Nishimura, T.; Hayashi, H.; Kuriki, T.
Glucosylation of acetic acid by sucrose phosphorylase
Biosci. Biotechnol. Biochem.
72
82-87
2008
Streptococcus mutans
Lee, J.H.; Moon, Y.H.; Kim, N.; Kim, Y.M.; Kang, H.K.; Jung, J.Y.; Abada, E.; Kang, S.S.; Kim, D.
Cloning and expression of the sucrose phosphorylase gene from Leuconostoc mesenteroides in Escherichia coli
Biotechnol. Lett.
30
749-754
2008
Leuconostoc mesenteroides, Leuconostoc mesenteroides NRRL B-742
Goedl, C.; Schwarz, A.; Mueller, M.; Brecker, L.; Nidetzky, B.
Mechanistic differences among retaining disaccharide phosphorylases: insights from kinetic analysis of active site mutants of sucrose phosphorylase and alpha,alpha-trehalose phosphorylase
Carbohydr. Res.
343
2032-2040
2008
Leuconostoc mesenteroides
Mueller, M.; Nidetzky, B.
Dissecting differential binding of fructose and phosphate as leaving group/nucleophile of glucosyl transfer catalyzed by sucrose phosphorylase
FEBS Lett.
581
3814-3818
2007
Leuconostoc mesenteroides
Sugimoto, K.; Nomura, K.; Nishiura, H.; Ohdan, K.; Kamasaki, H.; Nishimura, T.; Hayashi, H.; Kuriki, T.
Novel transglucosylating reaction of sucrose phosphorylase to carboxylic compounds
J. Appl. Glycosci.
55
119-125
2008
Streptococcus mutans
-
Sugimoto, K.; Nomura, K.; Nishiura, H.; Ohdan, K.; Ohdan, K.; Hayashi, H.; Kuriki, T.
Novel transglucosylating reaction of sucrose phosphorylase to carboxylic compounds such as benzoic acid
J. Biosci. Bioeng.
104
22-29
2007
Leuconostoc mesenteroides, Streptococcus mutans
Shin, M.H.; Jung, M.W.; Lee, J.; Kim, M.D.; Kim, K.H.
Strategies for producing recombinant sucrose phosphorylase originating from Bifidobacterium longum in Escherichia coli JM109
Process Biochem.
43
822-828
2008
Bifidobacterium longum
-
Goedl, C.; Sawangwan, T.; Wildberger, P.; Nidetzky, B.
Sucrose phosphorylase: A powerful transglucosylation catalyst for synthesis of alpha-D-glucosides as industrial fine chemicals
Biocatal. Biotransform.
28
10-21
2010
Leuconostoc mesenteroides, Pelomonas saccharophila, Streptococcus mutans, Bifidobacterium adolescentis (Q84HQ2), Leuconostoc mesenteroides B-1149
-
Abada, E.; Osman, M.; Lee, J.; Kim, D.
Molecular cloning of the gene 1355SPase encoding a sucrose phosphorylase from the bacterium Leuconostoc mesenteroides B-1355
Biotechnology
7
463-468
2008
Leuconostoc mesenteroides (B0F411), Leuconostoc mesenteroides NRRL B-1355 (B0F411)
-
Goedl, C.; Nidetzky, B.
Sucrose phosphorylase harbouring a redesigned, glycosyltransferase-like active site exhibits retaining glucosyl transfer in the absence of a covalent intermediate
ChemBioChem
10
2333-2337
2009
Leuconostoc mesenteroides
Shin, M.; Cheong, N.; Lee, J.; Kim, K.
Transglucosylation of caffeic acid by a recombinant sucrose phosphorylase in aqueous buffer and aqueous-supercritical CO2 media
Food Chem.
115
1028-1033
2009
Bifidobacterium longum
-
Kasperowicz, A.; Stan-Glasek, K.; Guczynska, W.; Piknova, M.; Pristas, P.; Nigutova, K.; Javorsky, P.; Michalowski, T.
Sucrose phosphorylase of the rumen bacterium Pseudobutyrivibrio ruminis strain A
J. Appl. Microbiol.
107
812-820
2009
Pseudobutyrivibrio ruminis, Pseudobutyrivibrio ruminis A
Wildberger, P.; Todea, A.; Nidetzky, B.
Probing enzyme substrate interactions at the catalytic subsite of Leuconostoc mesenteroides sucrose phosphorylase with site-directed mutagenesis: The roles of Asp
Biocatal. Biotransform.
30
326-337
2012
Leuconostoc mesenteroides
-
Cerdobbel, A.; De Winter, K.; Desmet, T.; Soetaert, W.
Sucrose phosphorylase as cross-linked enzyme aggregate: improved thermal stability for industrial applications
Biotechnol. J.
5
1192-1197
2010
Bifidobacterium adolescentis
Luley-Goedl, C.; Sawangwan, T.; Brecker, L.; Wildberger, P.; Nidetzky, B.
Regioselective O-glucosylation by sucrose phosphorylase: a promising route for functional diversification of a range of 1,2-propanediols
Carbohydr. Res.
345
1736-1740
2010
Leuconostoc mesenteroides
Wildberger, P.; Luley-Goedl, C.; Nidetzky, B.
Aromatic interactions at the catalytic subsite of sucrose phosphorylase: their roles in enzymatic glucosyl transfer probed with Phe52->Ala and Phe52->Asn mutants
FEBS Lett.
585
499-504
2011
Leuconostoc mesenteroides
De Winter, K.; Soetaert, W.; Desmet, T.
An imprinted cross-linked enzyme aggregate (iCLEA) of sucrose phosphorylase: combining improved stability with altered specificity
Int. J. Mol. Sci.
13
11333-11342
2012
Bifidobacterium adolescentis
Wiesbauer, J.; Goedl, C.; Schwarz, A.; Brecker, L.; Nidetzky, B.
Substitution of the catalytic acid-base Glu237 by Gln suppresses hydrolysis during glucosylation of phenolic acceptors catalyzed by Leuconostoc mesenteroides sucrose phosphorylase
J. Mol. Catal. B
65
24-29
2010
Leuconostoc mesenteroides
-
Renirie, R.; Pukin, A.; Van Lagen, B.; Franssen, M.
Regio- and stereoselective glucosylation of diols by sucrose phosphorylase using sucrose or glucose 1-phosphate as glucosyl donor
J. Mol. Catal. B
67
219-224
2010
Leuconostoc mesenteroides
-
Cerdobbel, A.; De Winter, K.; Aerts, D.; Kuipers, R.; Joosten, H.; Soetaert, W.; Desmet, T.
Increasing the thermostability of sucrose phosphorylase by a combination of sequence- and structure-based mutagenesis
Protein Eng. Des. Sel.
24
829-834
2011
Bifidobacterium adolescentis
Kasperowicz, A.; Stan-Glasek, K.; Guczynska, W.; Pristas, P.; Javorsky, P.; Vandzurova, A.; Michalowski, T.
?-Fructofuranosidase and sucrose phosphorylase of rumen bacterium Pseudobutyrivibrio ruminis strain 3
World J. Microbiol. Biotechnol.
28
1271-1279
2012
Pseudobutyrivibrio ruminis, Pseudobutyrivibrio ruminis 3
Du, L.; Yang, H.; Huo, Y.; Wei, H.; Xu, Y.; Wei, Y.; Huang, R.
A novel sucrose phosphorylase from the metagenomes of sucrose-rich environment: isolation and characterization
World J. Microbiol. Biotechnol.
28
2871-2878
2012
uncultured bacterium (B8Y3Y0)
Aerts, D.; Verhaeghe, T.; Joosten, H.J.; Vriend, G.; Soetaert, W.; Desmet, T.
Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input
Biotechnol. Bioeng.
110
2563-2572
2013
Streptococcus mutans
Kraus, M.; Grimm, C.; Seibel, J.
Redesign of the active site of sucrose phosphorylase through a clash-induced cascade of loop shifts
ChemBioChem
17
33-36
2016
Bifidobacterium adolescentis (Q84HQ2), Bifidobacterium adolescentis
Rocha, J.M.; Caseli, L.
Adsorption and enzyme activity of sucrose phosphorylase on lipid Langmuir and Langmuir-Blodgett films
Colloids Surf. B Biointerfaces
116
497-501
2014
Leuconostoc mesenteroides
Teixeira, J.S.; Abdi, R.; Su, M.S.; Schwab, C.; Gaenzle, M.G.
Functional characterization of sucrose phosphorylase and scrR, a regulator of sucrose metabolism in Lactobacillus reuteri
Food Microbiol.
36
432-439
2013
Limosilactobacillus reuteri (M9ZS93), Limosilactobacillus reuteri, Limosilactobacillus reuteri LTH5448 (M9ZS93)
De Winter, K.; Verlinden, K.; Kren, V.; Weignerova¡, L.; Soetaert, W.; Desmet, T.
Ionic liquids as cosolvents for glycosylation by sucrose phosphorylase: balancing acceptor solubility and enzyme stability
Green Chem.
15
1949-1955
2013
Bifidobacterium adolescentis (Q84HQ2)
-
Morimoto, K.; Yoshihara, A.; Furumoto, T.; Takata, G.
Production and application of a rare disaccharide using sucrose phosphorylase from Leuconostoc mesenteroides
J. Biosci. Bioeng.
119
652-656
2015
Leuconostoc mesenteroides
Verhaeghe, T.; Diricks, M.; Aerts, D.; Soetaert, W.; Desmet, T.
Mapping the acceptor site of sucrose phosphorylase from Bifidobacterium adolescentis by alanine scanning
J. Mol. Catal. B
96
81-88
2013
Bifidobacterium adolescentis (Q84HQ2)
-
Renesteen, E.; Cahyo, F.; Malik, A.
Transglycosylation activity and characterization of recombinant sucrose phosphorylase from Leuconostoc mesenteroides MBFWRS-3(1) expressed in Escherichia coli
Int. J. App. Pharm.
12
264-267
2020
Leuconostoc mesenteroides (E2IHA5), Leuconostoc mesenteroides MBFWRS-3(1) (E2IHA5)
-
Bolivar, J.M.; Luley-Goedl, C.; Leitner, E.; Sawangwan, T.; Nidetzky, B.
Production of glucosyl glycerol by immobilized sucrose phosphorylase Options for enzyme fixation on a solid support and application in microscale flow format
J. Biotechnol.
257
131-138
2017
Leuconostoc mesenteroides
Li, Y.; Li, Z.; He, X.; Chen, L.; Cheng, Y.; Jia, H.; Yan, M.; Chen, K.
Characterisation of a Thermobacillus sucrose phosphorylase and its utility in enzymatic synthesis of 2-O-beta-D-glucopyranosyl-L-ascorbic acid
J. Biotechnol.
305
27-34
2019
Thermobacillus sp. ZCTH02-B1 (A0A1Y3Q6Q6)
Wang, M.; Wu, J.; Wu, D.
Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme
Microb. Cell Fact.
17
23
2018
Bifidobacterium adolescentis
Yao, D.; Fan, J.; Han, R.; Xiao, J.; Li, Q.; Xu, G.; Dong, J.; Ni, Y.
Enhancing soluble expression of sucrose phosphorylase in Escherichia coli by molecular chaperones
Protein Expr. Purif.
169
105571
2020
Thermoanaerobacterium thermosaccharolyticum (WP_094046414.1), Thermoanaerobacterium thermosaccharolyticum
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