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2.4.1.7: sucrose phosphorylase

This is an abbreviated version!
For detailed information about sucrose phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.7

Reaction

sucrose
+
phosphate
=
D-fructose
+
alpha-D-glucose 1-phosphate

Synonyms

1149SPase, 1355SPase, 742SPase, BiSP, disaccharide glucosyltransferase, LmSPase, More, SPase, sucrose glucosyltransferase, sucrose: orthophosphate, alpha-D-glucosyltransferase, sucrose: phosphate alpha-D-glucosyltransferase, unspase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.7 sucrose phosphorylase

Purification

Purification on EC 2.4.1.7 - sucrose phosphorylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
6 × His-tagged recombinant enzyme is purified by Ni-NTA affinity chromatography
less than 10% impurities
-
mutant enzymes D295N and D295E
-
native enzyme 10fold, partially by gel filtration
-
partial
purification of thermostable mutant T47S/S62P/Y77H/V128L/K140M/Q144R/N155S/D249G by heating at 65°C for 20 min with 20% sucrose. The purified enzyme can be directly employed for the production of amylose with further purification
-
purified using standard immobilised metal affinity chromatography, facilitated by the fused 6-histidine tag
recombinant enzyme from Escherichia coli
Q84HQ2
recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography
-
recombinant enzyme partially by anion exchange chromatography
-
recombinant enzyme partially from Escherichia coli via heat treatment removing the contaminating phosphatase activity
-
recombinant His-tagged wild-type and mutant enzyme from Escherichia coli strain DH10B by nickel affinity chromatography
-
recombinant His4-tagged enzyme from Escherichia coli
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide from Escherichia coli DH10B 7fold to homogeneity by metal affinity and hydrophobic interaction chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli Top10 cells by single step affinity chromatography
-
recombinant unspase from Escherichia coli strain XL-1 Blue by affinity chromatography
reconbinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography