2.4.1.7: sucrose phosphorylase
This is an abbreviated version!
For detailed information about sucrose phosphorylase, go to the full flat file.
Word Map on EC 2.4.1.7
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2.4.1.7
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mesenteroides
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leuconostoc
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bifidobacterium
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adolescentis
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phosphorylases
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transglucosylation
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synthesis
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alpha-d-glucose
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laminaribiose
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deglucosylation
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dextransucrase
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pseudobutyrivibrio
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ruminis
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kojibiose
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medicine
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industry
- 2.4.1.7
- mesenteroides
- leuconostoc
-
bifidobacterium
- adolescentis
- phosphorylases
-
transglucosylation
- synthesis
- alpha-d-glucose
- laminaribiose
-
deglucosylation
- dextransucrase
-
pseudobutyrivibrio
- ruminis
- kojibiose
- medicine
- industry
Reaction
Synonyms
1149SPase, 1355SPase, 742SPase, BiSP, disaccharide glucosyltransferase, LmSPase, More, SPase, sucrose glucosyltransferase, sucrose: orthophosphate, alpha-D-glucosyltransferase, sucrose: phosphate alpha-D-glucosyltransferase, unspase
ECTree
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Purification
Purification on EC 2.4.1.7 - sucrose phosphorylase
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6 × His-tagged recombinant enzyme is purified by Ni-NTA affinity chromatography
partial
purification of thermostable mutant T47S/S62P/Y77H/V128L/K140M/Q144R/N155S/D249G by heating at 65°C for 20 min with 20% sucrose. The purified enzyme can be directly employed for the production of amylose with further purification
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purified using standard immobilised metal affinity chromatography, facilitated by the fused 6-histidine tag
recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography
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recombinant enzyme partially by anion exchange chromatography
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recombinant enzyme partially from Escherichia coli via heat treatment removing the contaminating phosphatase activity
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recombinant His-tagged wild-type and mutant enzyme from Escherichia coli strain DH10B by nickel affinity chromatography
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recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide from Escherichia coli DH10B 7fold to homogeneity by metal affinity and hydrophobic interaction chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli Top10 cells by single step affinity chromatography
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recombinant unspase from Escherichia coli strain XL-1 Blue by affinity chromatography
reconbinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography