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2.4.1.66: procollagen glucosyltransferase

This is an abbreviated version!
For detailed information about procollagen glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.66

Reaction

UDP-alpha-D-glucose
+
[procollagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine
=
UDP
+
[procollagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine

Synonyms

collagen glucosyltransferase, collagen hydroxylysyl glucosyltransferase, galactosylhydroxylysine glucosyltransferase, galactosylhydroxylysyl glucosyltransferase, GGT, glucosyltransferase, uridine diphosphoglucose-collagen, LH3, LH3/PLOD3, lysyl hydroxylase, lysyl hydroxylase 3, More, multifunctional procollagen lysine hydroxylase and glycosyltransferase, PLOD3, UDP-glucose-collagen glucosyltransferase, UDP-glucose:galactosylhydroxylysine-collagen glucosyltransferase, uridine diphosphoglucose-collagen glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.66 procollagen glucosyltransferase

Purification

Purification on EC 2.4.1.66 - procollagen glucosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
14600fold
-
20fold
-
3110fold
-
39800fold from embryo
-
5000fold
-
50fold
-
5400fold from plasma, 4400fold from blood platelets
-
80fold: soluble enzyme
-
affinity chromatography
-
His-tagged recombinant LH3/GGT/GT and N-terminal 30kDa fragment A of LH3 with GGT activity expressed in High Five insect cells
-
His-tagged recombinant LH3/GGT/GT protein expressed in Escherichia coli
-
LH3-V5/His fusion protein is purified using nickel-nitrilotriacetic acid-agarose resin column chromatography
-
Ni-NTA-agarose
over 2000fold from whole embryo, 160fold from embryo cartilage
-
partial
partial, His-tagged recombinant LH/GGT/GT protein expressed in Escherichia coli
-
skin, partial, solubilized with Triton X-100
-
solubilized with 0.5 M NaCl, 2 M urea increases solubilization rate
-
solubilized with CHAPS, Brij-35
-
solubilized with Nonidet P-40
-
solubilized with Triton X-100