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2.4.1.66: procollagen glucosyltransferase

This is an abbreviated version!
For detailed information about procollagen glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.66

Reaction

UDP-alpha-D-glucose
+
[procollagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine
=
UDP
+
[procollagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine

Synonyms

collagen glucosyltransferase, collagen hydroxylysyl glucosyltransferase, galactosylhydroxylysine glucosyltransferase, galactosylhydroxylysyl glucosyltransferase, GGT, glucosyltransferase, uridine diphosphoglucose-collagen, LH3, LH3/PLOD3, lysyl hydroxylase, lysyl hydroxylase 3, More, multifunctional procollagen lysine hydroxylase and glycosyltransferase, PLOD3, UDP-glucose-collagen glucosyltransferase, UDP-glucose:galactosylhydroxylysine-collagen glucosyltransferase, uridine diphosphoglucose-collagen glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.66 procollagen glucosyltransferase

Engineering

Engineering on EC 2.4.1.66 - procollagen glucosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A361G
-
mutant with reduced GGT activity
A380V
-
mutant with reduced GGT activity
A453I
-
mutant with a remarkably reduced GGT activity
C132I
-
mutant with dramatically reduced GGT activity
D331V
-
little effect on GGT activity
E371T
-
mutant with reduced GGT activity
E547K
-
mutant with increased GGT activity
F131L
-
mutant with markedly reduced GGT activity
F372Y
-
little effect on GGT activity
H165S
-
mutant with reduced GGT activity
K482A
-
mutant with increased GGT activity
K674V
-
no effect on GGT activity
L124V
-
mutant with 40% inhibited GGT activity
L125V
-
mutant with 70% inhibited GGT activity
L192K
-
mutant with reduced GGT activity
L196I
-
mutant with markedly reduced GGT activity
L42K
-
mutant with increased GGT activity
L491M
-
mutant with little increased GGT activity
L562I
-
mutant with increased GGT activity
L591I
-
mutant with increased GGT activity
M406L
-
mutant with reduced GGT activity
M642L
-
mutant with increased GGT activity
M724A
-
little effect on GGT activity
P591N
-
no effect on GGT activity
Q613E
-
mutant with little increased GGT activity
S204C
-
mutant with reduced GGT activity
V622L
-
no effect on GGT activity
A464I
-
no effect on GGT activity
C144I
-
mutant with dramatically reduced GGT activity
D187A/D189A/D190A/D191A
-
the mutant shows unreduced lysyl hydroxylase 3 activity
D392A
-
little effect on GGT activity
D669A
-
the mutant shows no lysyl hydroxylase 3 activity
F143L
-
mutant with reduced GGT activity
L136V
-
mutant with increased GGT activity
L137V
-
mutant with increased GGT activity
L208I
-
mutant with markedly reduced GGT activity
M650L
-
no effect on GGT activity
N23S
-
lysyl hydroxylase 3 activity is reduced by nearly 50%
R729A
-
the mutant shows no lysyl hydroxylase 3 activity
additional information