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2.4.1.66: procollagen glucosyltransferase

This is an abbreviated version!
For detailed information about procollagen glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.66

Reaction

UDP-alpha-D-glucose
+
[procollagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine
=
UDP
+
[procollagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine

Synonyms

collagen glucosyltransferase, collagen hydroxylysyl glucosyltransferase, galactosylhydroxylysine glucosyltransferase, galactosylhydroxylysyl glucosyltransferase, GGT, glucosyltransferase, uridine diphosphoglucose-collagen, LH3, LH3/PLOD3, lysyl hydroxylase, lysyl hydroxylase 3, More, multifunctional procollagen lysine hydroxylase and glycosyltransferase, PLOD3, UDP-glucose-collagen glucosyltransferase, UDP-glucose:galactosylhydroxylysine-collagen glucosyltransferase, uridine diphosphoglucose-collagen glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.66 procollagen glucosyltransferase

Cloned

Cloned on EC 2.4.1.66 - procollagen glucosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a single gene codes for LH, GGT and GT activities
-
cDNA sequence of GGT/LH3
-
expressed in MDCK, COS-7 and HT-1080 cells
-
expressed in MEF cells
-
expression from HeLa cell lines and from transient HEK293 cells
full-length LH3 and the N-terminal 30 kDa fragment A with GGT activity is cloned, sequenced and expressed in High Five insect cells
-
LH cDNA with GGT activity is cloned and expressed in Escherichia coli BL21 (DE3) pLysS, a single gene codes for LH, GGT and GT activities
-
LH cDNA with GGT activity is expressed in Escherichia coli XL1-Blue, cDNA sequence of GGT/LH
-
LH3 cDNA with GGT activity is cloned and expressed in Sf9 insect cells and in Escherichia coli XL1-Blue
-
LH3-V5/His fusion protein is expressed in HEK-293 cells
-
overexpression in HT-1080 cells, expression of fragments in Escherichia coli
Plod3 gene encoding LH3/GGT is cloned and sequenced, gene structure and regulation, localized on chromosome 5