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2.4.1.5: dextransucrase

This is an abbreviated version!
For detailed information about dextransucrase, go to the full flat file.

Word Map on EC 2.4.1.5

Reaction

sucrose
+
[(1->6)-alpha-D-glucosyl]n
=
D-fructose
 +
[(1->6)-alpha-D-glucosyl]n+1

Synonyms

B-512F dextransucrase, B-512FMC dextransucrase, Cab3, CEP, DexT, dextran-sucrase, DS, DSase, DSR, Dsr S protein, DSR-F, DSR-S, DSRB742, DSRBCB4, DSRC39-2, DsrE563, DsrP, DSRS, DSRWC, DsrX, FT045B dextransucrase, glucansucrase, glucosyltransferase, glucosyltransferase, sucrose-1,6-alpha-glucan, glycosyltransferase R, Gtf, Gtf-DSM, GTFR, More, SGE, sucrose 6-glucosyltransferase, sucrose:1, 6-alpha-D-glucan 6-alpha-glucosyltransferase, sucrose:1,6-alpha-D-glucan-6-alpha-D-glucosyltransferase, Wc392-rDSR, WcCab3-DSR

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.5 dextransucrase

Storage Stability

Storage Stability on EC 2.4.1.5 - dextransucrase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified enzyme, half-life: 37.9 days
-20°C, purified enzyme, loss of 25% activity within 14 days at 30C
-
-20°C, purified enzyme, most stable at, half-life is 307 days, 95% activity remains afer 21 days
JX679020
-4°C, purified enzyme, half-life: 69.4 days
25°C, crude enzyme in cashew apple juice, pH 6.5, 48 h , quite stable
-
25°C, purified recombinant enzyme, 59% activity remaining after 4 days
-
30°C, purified enzyme, half-life: 10.33 h
4°C or -70°C, stable during extended storage
-
4°C, 0.1% bovine serum albumin, pH 5.2-8.5, stable for 24 h
-
4°C, pH 6.0-9.0, 4 mg/ml dextran, 24 h stable, without dextran the stable range is narrowed to pH 7.0-9.0
-
4°C, purified enzyme, half-life is 129 days, 89% activity remains afer 21 days
JX679020
4°C, purified enzyme, loss of 92% activity within 4 days at 30°C
-
4°C, purified recombinant enzyme, 50% activity remaining after 7 weeks
-
4°C, purified recombinant enzyme, in 20 mM sodium acetate, pH 5.4, about 10% activity loss after 6 days, and about 35% activity loss after 20 days
in a deep-freezer, 33% glycerol, 0.1% bovine serum albumin, less than 20% loss of activity after several months
-
on standing at 4°C for 30 days the native enzyme is dissociated into three inactive proteins
-
storage stability decreased by addition of dextranase
-
the free as well as immobilized enzyme are most stable at 4°C for more than 1 month
-