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2.4.1.5: dextransucrase

This is an abbreviated version!
For detailed information about dextransucrase, go to the full flat file.

Word Map on EC 2.4.1.5

Reaction

sucrose
+
[(1->6)-alpha-D-glucosyl]n
=
D-fructose
 +
[(1->6)-alpha-D-glucosyl]n+1

Synonyms

B-512F dextransucrase, B-512FMC dextransucrase, Cab3, CEP, DexT, dextran-sucrase, DS, DSase, DSR, Dsr S protein, DSR-F, DSR-S, DSRB742, DSRBCB4, DSRC39-2, DsrE563, DsrP, DSRS, DSRWC, DsrX, FT045B dextransucrase, glucansucrase, glucosyltransferase, glucosyltransferase, sucrose-1,6-alpha-glucan, glycosyltransferase R, Gtf, Gtf-DSM, GTFR, More, SGE, sucrose 6-glucosyltransferase, sucrose:1, 6-alpha-D-glucan 6-alpha-glucosyltransferase, sucrose:1,6-alpha-D-glucan-6-alpha-D-glucosyltransferase, Wc392-rDSR, WcCab3-DSR

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.5 dextransucrase

Cloned

Cloned on EC 2.4.1.5 - dextransucrase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged DSRC39-2 in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged glucansucrase in Escherichia coli
DSR-S protein is fused to a thioredoxin tag at the N-terminal extremity, and a 6*His tag at the C-terminal end and expressed in Escherichia coli
-
enzyme expression in the periplasmic space of seven different Escherichia coli strains with inducer molecules such as lactose or IPTG. Escherichia coli strain BL21-CodonPlus(DE3)-RIL exhibits the highest enzyme activity with lactose, method optimization with respect to culture conditions results in a 12fold increase in activity at 17°C and 7.5 mM lactose
expressed in Bacillus megaterium strain MS941 and strain WH320. At high cell density conditions and low growth rates MS941, in contrast to WH320, does not maintain a vegetative growth which is essential for the expression of the foreign dsrS gene by using the xylA promoter. It is conceivable that applications of a promoter which is highly active under nutrient-limited cultivation conditions is necessary, at least for MS941, for the overexpression of recombinant genes in such Bacillus megaterium fed-batch cultivation process
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli. Native enzyme produces mainly 6-linked glucopyranosylresidues, while Escherichia coli recombinant enzyme produces a glucan consisting of 70% 6-linked glucopyranosyl residues and 15% 3,6-glucopyranosyl residues
-
expression in the industrially relevant lactic acid bacterium Lactococcus lactis
expression of the His-tagged enzyme from strain B-512F in Escherichia coli
-
expression of the truncated mutant enzyme, B-512FMC dextransucrase, in Escherichia coli
-
expression of thioredoxin-His-tagged wild-type and trunacted mutant C-terminal domain GBD-7
-
expression of wild-type enzyme DSRBCB4 and of mutant fusion enzyme of dextransucrase and dextranase, DXSR, in Escherichia coli
gene dexYG, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
gene dexYG, expression in Escherichia coli strain BL21(DE3)
-
gene dexYG, lactose-inducible expresssion of the enzyme in Escherichia coli strain BL21(DE3). Method optimization, highest activity at 25°C and 1% lactose, overview
-
gene dexYG, overexpression in Escherichia coli strain BL21(DE3)
-
gene dsr, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Lactococcus lactis
gene DSRB742, expression of wild-type and DRN1-DRN4 mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
-
gene dsrBCB4, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene dsrE563, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS using expression vector pRSETC
gene dsrF, DNA and amino acid sequence determination and analysis, sequence comparison, expression of DSR-F in Escherichia coli strain JM109
gene dsrP, DNA and amino acid sequence determination and analysis, the enzyme sequence possess seven repeat units in the N-terminal region as well as five cell wall binding repeats in the C-terminal region, expression in Escherichia coli
-
gene dsrS, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 AI
gene dsrWC, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain Top10F' and expression in Escherichia coli strain BL21(DE3)
gene dsrX, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, expressed as a N-terminal His6-tagged protein Escherichia coli strain BL21(DE3), optimization of the recombinant expression system
-
gene dsxr, the recombinant chimeric dsxr encodes dextranase-dextransucrase, DXSR, is sequentially composed of the dextransucrase-encoding gene dsrBCB4 and the gene dex2, encoding the dextranase from Arthrobacter oxidans, UniProt ID A9UKG4, expression in Escherichia coli strain BL21(DE3)pLysS
gene gtf, recombinant expression in Escherichia coli strain Rosetta
gene gtfR, expression of wild-type and mutant enzymes in Escherichia coli strain XL10-Gold
-
gene LcDS, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain MC1061, expression in Escherichia coli strain BL21
genes HJ-P4 and HJ-P5, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree
-
genes HJ-S7 and HJ-S13, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree
-
recombinant enzyme expression in Escherichia coli strain BL21 (DE3)/pET28-dexYG
-
recombinant soluble extracellular enzyme expression in Lactococcus lactis
recombinantly produced in Bacillus megaterium and exported into the growth medium. For this purpose a plasmid-based xylose-inducible gene expression system is optimized via introduction of a multiple cloning site and an encoded optimal Bacillus megaterium ribosome binding site
the recombinant wild-type enzyme and mutant proteins are expressed from Escherichia coli BL21 star (DE3)
truncated forms of dsrE are cloned and expressed in Escherichia coli. The catalytic domain CD1 is specific for the synthesis of alpha-1,6 glucosidic bonds and CD2 only catalyzes the formation of alpha-1,2 linkage
two ORFs coding for strain B/110-1-2 dextransucrases, DNA and amino acid sequence determination and analysis, sequence comparisons