2.4.1.41: polypeptide N-acetylgalactosaminyltransferase

This is an abbreviated version!
For detailed information about polypeptide N-acetylgalactosaminyltransferase, go to the full flat file.

Reaction

Synonyms

(UDP)-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-glycoprotein, bgibmga008904, dGalNAc-T3, Eg-ppGalNAc-T1, GalNAc transferase, GalNAc-T, GalNAc-T1, GalNAc-T11, GalNAc-T12, GalNAc-T13, GalNAc-T14, GalNAc-T16, GalNAc-T18, GalNAc-T2, GalNAc-T3, GalNAc-T4, GalNAc-T5, GalNAc-T6, GalNAc-T7, GalNAc-transferase, GalNAc-transferase T1, GalNAc-transferase T2, GalNAcT4, GALNT1, GALNT11, GALNT12, Galnt13, GALNT14, Galnt18, GALNT2, GALNT3, Galnt4, GalNT5, glycopeptide-preferring polypeptide GalNAc transferase 10, glycoprotein acetylgalactosaminyltransferase, More, N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyl-transferase, N-acetylgalactosaminyltransferase, N-acetylgalactosaminyltransferase-like 1, NM_001079884, PGANT, PGANT1, PGANT2, PGANT3, PGANT4, PGANT5, PGANT6, PGANT7, PGANT8, polypeptide GalNAc transferase, polypeptide GalNAc transferase 2, polypeptide GalNAc-transferase, polypeptide GalNAc-transferase 2, polypeptide GalNAc-transferase-2, polypeptide GalNAcT, polypeptide N-acetylgalactosaminyl transferase-3, polypeptide N-acetylgalactosaminyltransferase, polypeptide N-acetylgalactosaminyltransferase 12, polypeptide N-acetylgalactosaminyltransferase 14, polypeptide N-acetylgalactosaminyltransferase 2, polypeptide N-acetylgalactosaminyltransferase 3, polypeptide N-acetylgalactosaminyltransferase 6, polypeptide N-acetylgalactosaminyltransferase-1, polypeptide-N-acetylgalactosamine transferase, polyppetide GalNAc transferase, pp-GalNAc-T13, pp-GalNAc-T15, pp-GalNAc-T2, pp-GaNTase, ppGalNAc T1, ppGalNAc T10, ppGalNAc T13, ppGalNAc T16, ppGalNAc T2, ppGalNAc T3, ppGalNAc T5, ppGalNAc T6, ppGalNAc-T, ppGalNAc-T1, ppGalNAc-T10, ppGalNAc-T12, ppGalNAc-T2, ppGalNAc-T3, ppGalNAc-T4, ppGalNAc-T5, ppGalNAc-T6, ppGalNAcT, ppGalNAcT or hT, ppGaNTase, ppGaNTase-T1, ppGaNTase-T11, ppGaNTase-T12, ppGaNTase-T3, ppGaNTase-T4, ppGaNTase-T5, ppGaNTase-T6, protein-UDP acetylgalactosaminyltransferase, UDP GalNAc:polypeptide N-acetylgalactosaminyltransferase, UDP N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyl transferase, UDP N-acetyl-alpha-Dgalactosamine:polypeptide N-acetylgalactosaminyl transferase, UDP-acetylgalactosamine-glycoprotein acetylgalactosaminyltransferase, UDP-acetylgalactosamine:peptide-N-galactosaminyltransferase, UDP-GalNAc polypeptide:GalNAc transferase-T2, UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferase, UDP-GalNAc polypeptides:N-acetyl-alpha-galactosaminyltransferase, UDP-GalNAc transferase, UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase, UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase-2, UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase-T1, UDP-GalNAc:polypeptide alphaN-acetylgalactosaminyltransferase, UDP-GalNAc:polypeptide GalNAc transferase, UDP-GalNAc:polypeptide N-acetylgalactosaminyl transferase, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 18, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 9, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13V1, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 16, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 17, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 18, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 19, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 20, UDP-N-acetyl-alpha-D-galactosaminyltransferase, UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase-6, UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T1, UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T10, UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T3, UDP-N-acetylgalactosamine polypeptide: N-acetylgalactosaminyl-transferase, UDP-N-acetylgalactosamine-glycoprotein N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine-protein N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine:kappa-casein polypeptide N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine:polypeptide N-acetyl-alpha-galactosaminyltransferase, UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine:protein N-acetylgalactosaminyl transferase, UDP-N-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase, UDP-polypeptide N-acetylgalactosaminyl transferase, uridine diphosphate-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3, uridine diphosphoacetylgalactosamine-glycoprotein acetylgalactosaminyltransferase, xGaltnl-1

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.41 polypeptide N-acetylgalactosaminyltransferase

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Results	in table
6	Activating Compound
996	AA Sequence
18	Application
1	CAS Registry Number
75	Cloned(Commentary)
15	Crystallization (Commentary)
1566	Disease/ Diagnostics
7	Expression
24	General Information
5	General Stability
47	IC50 Value
63	Inhibitors
3	kcat/KM [mM/s]
9	Ki Value [mM]
201	KM Value [mM]
58	Localization
60	Metals/Ions
26	Molecular Weight [Da]
74	Natural Substrates/ Products (Substrates)
158	Organism
5	Pathway
48	PDB ID
18	pH Optimum
3	pH Range
10	Posttranslational Modification
112	Protein Variants
49	Purification (Commentary)
3	Reaction
11	Reaction Type
142	Reference
320	Source Tissue
85	Specific Activity [micromol/min/mg]
7	Storage Stability
569	Substrates and Products (Substrate)
11	Subunits
296	Synonyms
1	Systematic Name
37	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
42	Turnover Number [1/s]

Purification

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Purification on EC 2.4.1.41 - polypeptide N-acetylgalactosaminyltransferase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

5 entries

1320fold

Sus scrofa

-

489170

21fold, recombinant soluble enzyme, expressed in Sf9 cells

Bos taurus

-

489175

2500fold

Mus musculus

-

489172

48100fold

Rattus norvegicus

-

489167

517000fold

Bos taurus

-

489172

7500fold

Bos taurus

-

489168

affinity chromatography on deglycosylated Cowper's gland mucin bound to Sepharose, 12500fold

Sus scrofa

-

489177

anti-FLAG m2 affinity chromatography

2 entries

Co2+-charged resin column chromatography

Homo sapiens

-

735737

GST fusion protein purified by affinity chromatography, elution with 10 mM glutathione, pH8

Homo sapiens

Q96FL9

689866

GSTrap FF chromatography

Homo sapiens

Q96FL9

689866

immobilized metal ion affinity chromatography (Ni2+)

Homo sapiens

-

718747

ion exchange chromatography

Homo sapiens

-

719568

Ni-NTA-agarose column chromatography and MonoQ column chromatography

Homo sapiens

-

736418

partial

2 entries

placenta enzyme, Muc2 peptide affinity chromatography separates two distinct transferase activities with overlapping specificity concerning Muc2 substrate, but which are distinguishable with respect to HIV-V3 substrate

Homo sapiens

-

489178

ppGalNAc-T13, -T1 and -T9, expressed in Sf21 or High FiveTM insect cells

Homo sapiens

Q8IUC8

489184

ppGalNAc-T14 and -T2, expressed in Sf21 cells

Homo sapiens

-

489183

purification of His-tagged truncated lectin domain by IMAC, 250 mM imidazole

Homo sapiens

Q10471, Q10472, Q8N4A0, Q8NCW6

686971

purification of secreted recombinant proteins from cell culture supernatant by Amberlite IRA-95 chromatography followed by SP Sepharose Fast Flow ion-exchange chromatography and concentrated by centrifugation through 10000 Da cut-off filter followed by elution from a PC3.2/3 column

Homo sapiens

Q10471, Q10472, Q8N4A0, Q8NCW6

686971

purified to apparent homogeneity using a synthetic acceptor peptide as affinity ligand

Homo sapiens

Q10471, Q10472

659127

recombinant enzyme

4 entries

recombinant enzyme, expressed in COS7 cells

Drosophila melanogaster

Q8MVS5

489191

recombinant from Drosophila melanogaster S2 cells

Toxoplasma gondii

Q8MM26

489194

recombinant GalNAc-T1, -T2 and -T3, expressed in Sf9 cells

Homo sapiens

-

489179

recombinant His-tagged isozymes ppGalNAcT-1 and ppGalNAcT-2 from Pichia pastoris medium by nickel affinity chromatography, the tag is cleaved off by TEV protease, recombinant isozyme ppGalNAcT-3t from medium of COS-7 cells

Mus musculus

-

704654

recombinant mutant GalNAc-T1, expressed in COS7 cells

Rattus norvegicus

-

489190

recombinant ppGalNAc-T1, -T2, -T3, -T4, -T6 and –T9, expressed in insect cells

Homo sapiens

-

489188

recombinant ppGaNTases expressed in COS7 cells

Mus musculus

-

489189

recombinant soluble His-tagged enzyme from Cos-7 cells, purification from the medium

Bos taurus

-

489200

recovery of the secreted recombinant proteins that harbour a protein A IgG-binding domain by IgG-Sepharose

Rattus norvegicus

Q10473

686681

SP-Sepharose column chromatography and MonoQ or MonoS column chromatography

Homo sapiens

-

736203

submaxillary gland enzyme, 123.5fold

Ovis sp.

-

489178

submaxillary gland enzyme, 25.4fold

Sus scrofa

-

489178

-

Sus scrofa

-

489169, 489171, 489201

-

Bos taurus

-

489176, 489186, 489201

-

Bos taurus

Q07537

659126

-

Homo sapiens

Q10471, Q10472, Q8N4A0

659136

-

Mus musculus

O08912

660363

anti-FLAG m2 affinity chromatography

Homo sapiens

-

719570

anti-FLAG m2 affinity chromatography

Homo sapiens

Q58A54

719569

partial

Mus musculus

-

489172, 489189

partial

Homo sapiens

-

489187

recombinant enzyme

Drosophila sp. (in: flies)

-

658238

recombinant enzyme

Sus scrofa

-

659929

recombinant enzyme

Bos taurus

-

660415

recombinant enzyme

Toxoplasma gondii

Q6YBY0

657665