2.4.1.4: amylosucrase
This is an abbreviated version!
For detailed information about amylosucrase, go to the full flat file.
Word Map on EC 2.4.1.4
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2.4.1.4
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neisseria
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polysaccharea
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deinococcus
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geothermalis
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synthesis
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food industry
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biotechnology
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amylose-like
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transglucosylation
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waxy
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drug development
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turanose
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asases
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transglucosidase
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amylopectin
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c-myc-binding
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maltooligosaccharides
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glycoside-hydrolase
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trehalulose
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industry
- 2.4.1.4
- neisseria
- polysaccharea
-
deinococcus
- geothermalis
- synthesis
- food industry
- biotechnology
-
amylose-like
-
transglucosylation
-
waxy
- drug development
- turanose
- asases
- transglucosidase
- amylopectin
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c-myc-binding
- maltooligosaccharides
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glycoside-hydrolase
- trehalulose
- industry
Reaction
Synonyms
AaAS, ACAS, AmAS, AMS, Amy-1, ASASE, BtAS, CcAS, DGAS, DRAS, DRpAS, glucosyltransferase, sucrose-1,4-alpha-glucan, MaAS, MFAS, More, NPAS, NsAS, sucrose-glucan glucosyltransferase, SyAS
ECTree
2.4.1.4 amylosucraseAdvanced search results
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results (Natural Substrates Products
Natural Substrates Products on EC 2.4.1.4 - amylosucrase
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NATURAL SUBSTRATE 
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose + phloretin
D-fructose + phloretin glucoside A1 + phloretin glucoside A2 + phloretin glucoside A3
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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involved in biosynthesis of amylopectin-glycogen type polysaccharide
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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amylosucrase initializes polymer formation by releasing, through sucrose hydrolysis, a glucose molecule that is subsequently used as the first acceptor molecule. Maltooligosaccharides of increasing size are produced and successively elongated at their nonreducing ends until they reached a critical size and concentration, causing precipitation
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-
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + phloretin
D-fructose + phloretin glucoside A1 + phloretin glucoside A2 + phloretin glucoside A3
the enzyme is a non-Leloir glycosyltransferase that catalyzes the stereospecific glucosylation of phloretin at the 4'-position. Phloretin and its glucosylation derivatives are cytotoic, overview
three major phloretin-dependent sugar-positive products are observed containing one to three Glc residues (Phlo-A1, -A2, -A3), identification by TLC and NMR spectrometry. In all three metabolites the first Glc, GlcA, is linked to the aglycone at C4'
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?
sucrose + phloretin
D-fructose + phloretin glucoside A1 + phloretin glucoside A2 + phloretin glucoside A3
the enzyme is a non-Leloir glycosyltransferase that catalyzes the stereospecific glucosylation of phloretin at the 4'-position. Phloretin and its glucosylation derivatives are cytotoic, overview
three major phloretin-dependent sugar-positive products are observed containing one to three Glc residues (Phlo-A1, -A2, -A3), identification by TLC and NMR spectrometry. In all three metabolites the first Glc, GlcA, is linked to the aglycone at C4'
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?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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amylosucrases catalyze the formation of an alpha-1,4-glucosidic linkage by transferring a glucosyl unit from sucrose onto an acceptor alpha-1,4-glucan
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-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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linear alpha-(1,4)-glucans
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?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
the enzyme catalyzes the synthesis of alpha-1,4 glucans from sucrose. The product profile is quite polydisperse, ranging from soluble chains called maltooligosaccharides to high-molecular weight insoluble amylose
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-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
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-
-
?
additional information
?
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amylosucrase is a transglucosidase that catalyses the synthesis of an amylose-type polymer from sucrose, an abundant agro-resource
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-
?
additional information
?
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the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose
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?
additional information
?
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amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate
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?
additional information
?
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amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate
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?
additional information
?
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amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate
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?
additional information
?
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amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate
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?
additional information
?
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the enzyme catalyzes the synthesis of a water-insoluble amylose-like polymer from sucrose, a readily available and low-cost agroresource
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?
additional information
?
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the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose
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-
?
additional information
?
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the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose
-
-
?
additional information
?
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amylosucrase (AS), a glucosyltransferase from Neiserria polysaccharea, produces an insoluble alpha-1,4-linked glucan polymer by consuming sucrose and releasing fructose. This reaction does not require a-D-glucosyl-nucleotide-diphosphate like ADP- or UDP-glucose, but rather uses the energy generated by splitting sucrose in order to synthesise the glucan polymer
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?
additional information
?
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amylosucrase from Neisseria polysaccharea is a transglucosylase that synthesizes an insoluble amylose-like polymer from sole substrate sucrose, product isolation and analysis, overview
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?
additional information
?
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amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate
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-
?
additional information
?
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amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate
-
-
?
additional information
?
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the amylosucrase from Neisseria polysaccharea naturally catalyzes the synthesis of alpha-glucans from the widely available donor sucrose. NpAS is highly specific for its natural substrate and subsite -1 (according to GH nomenclature) plays a major role in the recognition of the sucrose glucosyl moiety through a highly efficient hydrogen bonding interaction network, subsite 21 is responsible for the high affinity for sucrose
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-
?
additional information
?
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the amylosucrase from Neisseria polysaccharea naturally catalyzes the synthesis of alpha-glucans from the widely available donor sucrose. NpAS is highly specific for its natural substrate and subsite -1 (according to GH nomenclature) plays a major role in the recognition of the sucrose glucosyl moiety through a highly efficient hydrogen bonding interaction network, subsite 21 is responsible for the high affinity for sucrose
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?
additional information
?
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treatment of pre-gelatinized rice and barley starches with amylosucrase from Neisseria polysaccharea for resistant starch production. Analysis of reaction efficiency, resistant starch content, amylopectin branch-chain length distribution, solubility, welling power, pasting viscosity, and thermal transition properties, detailed overview
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?
additional information
?
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the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose
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?
additional information
?
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the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose
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?
