2.4.1.321: cellobionic acid phosphorylase
This is an abbreviated version!
For detailed information about cellobionic acid phosphorylase, go to the full flat file.
Word Map on EC 2.4.1.321
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2.4.1.321
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1-phosphate
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aldonic
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phosphorolysis
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crassa
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neurospora
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cellulolytic
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synthesis
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polysaccharide
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glycoside
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lytic
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biomass
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pentose
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phosphorylases
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degradans
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saccharophagus
- 2.4.1.321
- 1-phosphate
-
aldonic
-
phosphorolysis
- crassa
-
neurospora
-
cellulolytic
- synthesis
- polysaccharide
- glycoside
-
lytic
- biomass
- pentose
- phosphorylases
- degradans
-
saccharophagus
Reaction
Synonyms
4-O-beta-D-glucopyranosyl-D-gluconic acid: phosphate alpha-D-glucosyltransferase, CAP, CBAP, cep94B, NCU09425, NdvB, Sde_0906, XCC4077
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Application
Application on EC 2.4.1.321 - cellobionic acid phosphorylase
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synthesis
an engineered strain of Neurospora crassa (F5) with six of seven beta-glucosidase (bgl) genes knocked out produces cellobiose and cellobionate directly from cellulose without the addition of exogenous cellulases. Further modification by knock-out of catabolite repression genes, cre-1 and ace-1, leads to improved cellobiose dehydrogenase and exoglucanase expression but not to an improvement in cellobiose or cellobionate production. Deletion of the cellobionate phosphorylase gene NdvB from the genome of F5 lacking ace-1 and cre-1 to prevent the consumption of cellobiose and cellobionate results in a slightly reduced hydrolysis rate and in convertsion of 75% of the cellulose consumed to the desired products, cellobiose and cellobionate, compared to 18% converted by the strain F5 lackong only ace-1 cre-1
synthesis
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engineering of recombinant Neurospora crassa strains to produce cellobionate. Recombinant strains heterologously express laccase genes from different sources in Neurospora crassa which has six out of seven beta-glucosidase, two transcription factors, and the cellobionate phosphorylase (ndvB) genes deleted. The engineered strain produces 47.4 mM cellobionate from cellulose without any enzyme addition
synthesis
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engineering of recombinant Neurospora crassa strains to produce cellobionate. Recombinant strains heterologously express laccase genes from different sources in Neurospora crassa which has six out of seven beta-glucosidase, two transcription factors, and the cellobionate phosphorylase (ndvB) genes deleted. The engineered strain produces 47.4 mM cellobionate from cellulose without any enzyme addition
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synthesis
-
an engineered strain of Neurospora crassa (F5) with six of seven beta-glucosidase (bgl) genes knocked out produces cellobiose and cellobionate directly from cellulose without the addition of exogenous cellulases. Further modification by knock-out of catabolite repression genes, cre-1 and ace-1, leads to improved cellobiose dehydrogenase and exoglucanase expression but not to an improvement in cellobiose or cellobionate production. Deletion of the cellobionate phosphorylase gene NdvB from the genome of F5 lacking ace-1 and cre-1 to prevent the consumption of cellobiose and cellobionate results in a slightly reduced hydrolysis rate and in convertsion of 75% of the cellulose consumed to the desired products, cellobiose and cellobionate, compared to 18% converted by the strain F5 lackong only ace-1 cre-1
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