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2.4.1.288: galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase

This is an abbreviated version!
For detailed information about galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.288

Reaction

28 UDP-alpha-D-galactofuranose +

beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
= 28 UDP +
[beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol

Synonyms

galactofuranosyl transferase 2, galactofuranosyltransferase, galactosyl transferase, GalTr, GlfT, GlfT2, LmjF.25.0010, LMJF_25_0010, LPG1, LPG1G, LPG1G2, LPG1L, Rv3808c

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.288 galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase

Engineering

Engineering on EC 2.4.1.288 - galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D256A
-
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
D256E
-
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
D258A
-
the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+
D258E
-
the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme
D372S
the mutant shows no detectable activity
E300S
the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme
E371S
the mutant shows no detectable activity
H413S
the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme
W399S
the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme
D256A
-
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
-
D256E
-
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
-
D258A
-
the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+
-
D258E
-
the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme
-