2.4.1.27: DNA beta-glucosyltransferase

This is an abbreviated version!
For detailed information about DNA beta-glucosyltransferase, go to the full flat file.

Reaction

Synonyms

beta-GT, BGT, glucosyltransferase, uridine diphosphoglucose-deoxyribonucleate beta-, GT-B glycosyltransferase, More, T4 beta-glucosyl transferase, T4 beta-glucosyltransferase, T4 phage beta-glucosyltransferase, T4-beta-glucosyl transferase, T4-HMC-beta-glucosyl transferase, UDP glucose-DNA beta-glucosyltransferase, uridine diphosphoglucose-deoxyribonucleate beta-glucosyltransferase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.27 DNA beta-glucosyltransferase

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Results	in table
2	AA Sequence
2	Application
1	CAS Registry Number
1	Cloned(Commentary)
8	Crystallization (Commentary)
3	IC50 Value
5	Inhibitors
5	kcat/KM [mM/s]
4	Ki Value [mM]
6	KM Value [mM]
3	Metals/Ions
8	Natural Substrates/ Products (Substrates)
12	Organism
24	PDB ID
4	Reaction
1	Reaction Type
12	Reference
1	Source Tissue
23	Substrates and Products (Substrate)
1	Subunits
14	Synonyms
1	Systematic Name
5	Turnover Number [1/s]

Substrates Products

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of the Enzyme Summary Page.

Substrates Products on EC 2.4.1.27 - DNA beta-glucosyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

12 entries

UDP-alpha-D-glucose + 5-hydroxymethylcytosine-containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine-containing DNA

Tequatrovirus T4

-

-

758468

-

-

-

UDP-D-glucose + 5-hydroxymethyl cytosine

UDP + 5-hydroxymethyl cytosine beta-D-glucoside

Tequatrovirus T4

-

5-hydroxymethyl cytosine residues of duplex DNA

659730

-

-

?

UDP-D-glucose + 5-hydroxymethyl cytosine containing DNA

UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing DNA

Tequatrovirus T4

-

-

718919

recombinant enzyme glucosylates 5-hydroxymethyl cytosine containing DNA in a nonprocessive manner, and binding to either 5-hydroxymethyl cytosine containing DNA or uridine diphosphoglucose substrates is random, with both binary complexes being catalytically competent. The numbers of 5-hydroxymethyl cytosine on target sequences influence the turnover numbers for the recombinant enzyme

-

?

UDP-D-glucose + 5-hydroxymethyl cytosine containing phage T4 DNA

UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing phage T4 DNA

Tequatrovirus T4

-

-

718919

-

-

?

UDP-D-glucose + DNA containing 12 5-hydroxymethyl cytosine residues

UDP + ?

Tequatrovirus T4

-

-

718919

-

-

?

UDP-D-glucose + DNA containing 2 5-hydroxymethyl cytosine residues

UDP + ?

Tequatrovirus T4

-

-

718919

-

-

?

UDP-D-glucose + DNA containing 24 5-hydroxymethyl cytosine residues

UDP + ?

Tequatrovirus T4

-

-

718919

-

-

?

UDP-D-glucose + DNA containing 6 5-hydroxymethyl cytosine residues

UDP + ?

Tequatrovirus T4

-

-

718919

-

-

?

additional information

?

-

3 entries

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

-

-

758470

-

-

-

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

-

-

489027

-

489027

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

-

-

489029

-

489029

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

-

-

489030

-

489030

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

-

-

489031

-

489031

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

-

-

489033

-

489033

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

P04547

-

489028

-

489028

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

P04547

-

489032

-

489032

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

-

DNA-modifying enzyme

489031

-

-

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

-

the enzyme forms part of a phage DNA protection system

489030

-

489030

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

-

the enzyme is part of a system by which T-even bacteriophages glycosylate their genomic DNA in order to protect it against nuclease attack

489033

-

-

?

UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA

UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA

Tequatrovirus T4

P04547

the enzyme modifies T4-DNA

489032

-

-

?

additional information

?

-

Tequatrovirus T4

-

the AGT with GT-B glycosyltransferase fold is responsible for DNA base flipping and substrate recognition, mechanism, overview

659730

-

-

?

additional information

?

-

Tequatrovirus T4

-

the alpha-linkage forming enzyme AGT is also able to adopt the fold of a GT-B glycosyltransferase keeping the sugar donor binding mechanism typical for GT-B glycosyltransferases, but showing a unique sugar acceptor binding mode, both substrates are bound to the C-terminal domain, detailed substrate binding structures, catalytic mechanism of both enzyme types, overview

659730

-

-

?

additional information

?

-

Tequatrovirus T4

-

enzyme shows strong preference for the glucosylation of DNA containing symmetrical 5-hydroxymethyl cytosine resiudes over hemi-5-hydroxymethyl cytosine. The enzyme is capable of saturation glucosylation on all conformations of 5-hydroxymethyl cytosine.The numbers of 5-hydroxymethyl cytosine residues on DNA can influence catalysis, especially the rate of catalysis, and hemihydroxymethylated DNA is not the preferred substrate of the enzyme

718919

-

-

?