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0.0052
glycolic acid
-
pH 7.8, 25°C, cosubstrate: GDP-mannose
0.017
D-glycerate
mutant T139A, pH 7.0, 23°C, presence of Mn2+
0.023
D-glycerate
mutant E166A, pH 7.0, 23°C, presence of Mn2+
0.023
D-glycerate
mutant Q66A, pH 7.0, 23°C, presence of Mn2+
0.032
D-glycerate
wild-type, pH 7.0, 23°C, presence of Mn2+
0.055
D-glycerate
mutant R73A, pH 7.0, 23°C, presence of Mn2+
0.06
D-glycerate
mutant R73G, pH 7.0, 23°C, presence of Mn2+
0.065
D-glycerate
mutant D135A, pH 7.0, 23°C, presence of Mn2+
0.067
D-glycerate
mutant Y220A, pH 7.0, 23°C, presence of Mn2+
0.07
D-glycerate
mutant Y220F, pH 7.0, 23°C, presence of Mn2+
0.075
D-glycerate
mutant K76A, pH 7.0, 23°C, presence of Mn2+
0.077
D-glycerate
mutant W189A, pH 7.0, 23°C, presence of Mn2+
0.083
D-glycerate
mutant H217A, pH 7.0, 23°C, presence of 3 mM Mg2+
0.115
D-glycerate
mutant K9A, pH 7.0, 23°C, presence of Mn2+
0.135
D-glycerate
mutant M229A, pH 7.0, 23°C, presence of Mn2+
0.183
D-glycerate
mutant Y37A, pH 7.0, 23°C, presence of Mn2+
0.25
D-glycerate
mutant R218A, pH 7.0, 23°C, presence of Mn2+
0.267
D-glycerate
mutant H217A, pH 7.0, 23°C, presence of Mn2+
0.6
D-glycerate
wild-type, pH 7.0, 23°C, presence of 3 mM Ca2+
1.02
D-glycerate
-
pH 7.8, 25°C, cosubstrate: GDP-mannose
1.92
D-glycerate
wild-type, pH 7.0, 23°C, presence of 3 mM Mg2+
6.5
D-glycerate
-
pH 7.8, 65°C, cosubstrate: GDP-mannose
0.0026
D-lactate
-
pH 7.8, 25°C, cosubstrate: GDP-mannose
0.017
D-lactate
wild-type, pH 7.0, 23°C, presence of Mn2+
0.63
GDP-glucose
-
pH 7.8, 25°C, cosubstrate: D-glycerate
1.1
GDP-glucose
-
pH 7.8, 25°C, cosubstrate: D-glycerate
0.018
GDP-mannose
wild-type, pH 7.0, 23°C, presence of Mn2+
0.028
GDP-mannose
mutant E166A, pH 7.0, 23°C, presence of Mn2+
0.037
GDP-mannose
mutant Y220F, pH 7.0, 23°C, presence of Mn2+
0.042
GDP-mannose
mutant Y220A, pH 7.0, 23°C, presence of Mn2+
0.045
GDP-mannose
mutant D135A, pH 7.0, 23°C, presence of Mn2+
0.047
GDP-mannose
mutant R73A, pH 7.0, 23°C, presence of Mn2+
0.06
GDP-mannose
mutant R73G, pH 7.0, 23°C, presence of Mn2+
0.067
GDP-mannose
mutant W189A, pH 7.0, 23°C, presence of Mn2+
0.068
GDP-mannose
mutant K76A, pH 7.0, 23°C, presence of Mn2+
0.082
GDP-mannose
mutant K9A, pH 7.0, 23°C, presence of Mn2+
0.085
GDP-mannose
mutant T139A, pH 7.0, 23°C, presence of Mn2+
0.127
GDP-mannose
mutant M229A, pH 7.0, 23°C, presence of Mn2+
0.163
GDP-mannose
mutant Y37A, pH 7.0, 23°C, presence of Mn2+
0.183
GDP-mannose
mutant R218A, pH 7.0, 23°C, presence of Mn2+
0.483
GDP-mannose
mutant H217A, pH 7.0, 23°C, presence of Mn2+
0.967
GDP-mannose
mutant Q66A, pH 7.0, 23°C, presence of Mn2+
1.02
GDP-mannose
-
pH 7.8, 25°C, cosubstrate: D-glycerate
6.1
GDP-mannose
-
pH 7.8, 65°C, cosubstrate: D-glycerate
2.4.1.269 mannosylglycerate synthase
results (
results (
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