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0.0014 - 121.9
D-glycerate
124.9 - 138.6
GDP-glucose
0.00031 - 89.4
GDP-mannose
23.7
glycolic acid
-
pH 7.8, 25°C, cosubstrate: GDP-mannose
0.0014
D-glycerate
mutant K9A, pH 7.0, 23°C, presence of Mn2+
0.0022
D-glycerate
wild-type, pH 7.0, 23°C, presence of Mn2+
0.0038
D-glycerate
mutant R73A, pH 7.0, 23°C, presence of Mn2+
0.0041
D-glycerate
mutant R73G, pH 7.0, 23°C, presence of Mn2+
0.0058
D-glycerate
mutant R218A, pH 7.0, 23°C, presence of Mn2+
0.0078
D-glycerate
mutant M229A, pH 7.0, 23°C, presence of Mn2+
0.015
D-glycerate
mutant W189A, pH 7.0, 23°C, presence of Mn2+
0.017
D-glycerate
mutant Y37A, pH 7.0, 23°C, presence of Mn2+
0.03
D-glycerate
mutant K76A, pH 7.0, 23°C, presence of Mn2+
0.032
D-glycerate
mutant E166A, pH 7.0, 23°C, presence of Mn2+
0.07
D-glycerate
mutant Q66A, pH 7.0, 23°C, presence of Mn2+
0.6
D-glycerate
pH 7.6, 90°C, native enzyme
0.9
D-glycerate
pH 7.6, 90°C, recombinant enzyme
1.1
D-glycerate
mutant Y220A, pH 7.0, 23°C, presence of Mn2+
1.2
D-glycerate
-
cosubstrate GDP-mannose, pH 8.0, 25°C
1.3
D-glycerate
mutant D135A, pH 7.0, 23°C, presence of Mn2+
1.6
D-glycerate
-
cosubstrate GDP-glucose, pH 8.0, 25°C
1.9
D-glycerate
mutant H217A, pH 7.0, 23°C, presence of Mn2+
3.3
D-glycerate
mutant T139A, pH 7.0, 23°C, presence of Mn2+
3.4
D-glycerate
-
cosubstrate GDP-glucose, pH 8.0, 50°C
3.8
D-glycerate
mutant Y220F, pH 7.0, 23°C, presence of Mn2+
4.4
D-glycerate
-
cosubstrate GDP-mannose, pH 8.0, 50°C
96.5
D-glycerate
-
pH 7.8, 65°C, cosubstrate: GDP-mannose
121.9
D-glycerate
-
pH 7.8, 25°C, cosubstrate: GDP-mannose
0.0022
D-lactate
wild-type, pH 7.0, 23°C, presence of Mn2+
21.3
D-lactate
-
pH 7.8, 25°C, cosubstrate: GDP-mannose
124.9
GDP-glucose
-
pH 7.8, 25°C, cosubstrate: D-glycerate
138.6
GDP-glucose
-
pH 7.8, 25°C, cosubstrate: D-glycerate
0.00031
GDP-mannose
mutant T139A, pH 7.0, 23°C, presence of Mn2+
0.001
GDP-mannose
mutant Q66A, pH 7.0, 23°C, presence of Mn2+
0.001
GDP-mannose
mutant R73A, pH 7.0, 23°C, presence of Mn2+
0.0017
GDP-mannose
mutant K9A, pH 7.0, 23°C, presence of Mn2+
0.0027
GDP-mannose
mutant Y37A, pH 7.0, 23°C, presence of Mn2+
0.0028
GDP-mannose
mutant M229A, pH 7.0, 23°C, presence of Mn2+
0.0028
GDP-mannose
mutant W189A, pH 7.0, 23°C, presence of Mn2+
0.0029
GDP-mannose
mutant Y220A, pH 7.0, 23°C, presence of Mn2+
0.0031
GDP-mannose
wild-type, pH 7.0, 23°C, presence of Mn2+
0.0041
GDP-mannose
mutant R73G, pH 7.0, 23°C, presence of Mn2+
0.0063
GDP-mannose
mutant D135A, pH 7.0, 23°C, presence of Mn2+
0.0065
GDP-mannose
mutant Y220F, pH 7.0, 23°C, presence of Mn2+
0.0096
GDP-mannose
mutant E166A, pH 7.0, 23°C, presence of Mn2+
0.011
GDP-mannose
mutant K76A, pH 7.0, 23°C, presence of Mn2+
0.012
GDP-mannose
mutant H217A, pH 7.0, 23°C, presence of Mn2+
0.033
GDP-mannose
mutant R218A, pH 7.0, 23°C, presence of Mn2+
0.2
GDP-mannose
-
pH 8.0, 25°C
0.2
GDP-mannose
pH 7.6, 90°C, recombinant enzyme
0.3
GDP-mannose
pH 7.6, 90°C, native enzyme
0.6
GDP-mannose
-
pH 8.0, 25°C
1
GDP-mannose
-
pH 8.0, 50°C
2.4
GDP-mannose
-
pH 8.0, 50°C
81.2
GDP-mannose
-
pH 7.8, 25°C, cosubstrate: D-glycerate
89.4
GDP-mannose
-
pH 7.8, 65°C, cosubstrate: D-glycerate