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2.4.1.269: mannosylglycerate synthase

This is an abbreviated version!
For detailed information about mannosylglycerate synthase, go to the full flat file.

Word Map on EC 2.4.1.269

Reaction

GDP-alpha-D-mannose
+
D-glycerate
=
GDP
 +
2-O-(alpha-D-mannopyranosyl)-D-glycerate

Synonyms

MGS

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.269 mannosylglycerate synthase

Engineering

Engineering on EC 2.4.1.269 - mannosylglycerate synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D100A
-
inactive mutant enzyme
D102A
D135A
very high Km value for D-glycerate and a slight increase in kcat
E166A
15-fold increase in Km for glycerate
H217A
K76A
3-4-fold increase in kcat coupled with an elevation in Km for D-glycerate and GDP-Man of 15- and 3-fold, respectively
K9A
significant increase in kcat, little effect on the Km value for GDP-Man
M229A
minor influence on catalytic performance
Q66A
substantial increases in kcat and Km for both the acceptor and donor substrates
R131A
-
inactive mutant enzyme
R218A
significant increase in kcat and Km values for GDP-Man, only a modest influence on the Km value for the acceptor substrate
R73A
minor influence on catalytic performance
R73G
minor influence on catalytic performance
T139A
Km value for lactate above 100 mM, modest decrease in kcat but a 1500-fold increase in the Km values for D-glycerate
W189A
3- and 7-fold increase in kcat and Km for D-glycerate, compared with the wild-type enzyme, no influence on utilization of GDP-Man
Y220A
500-fold increase in Km for D-glycerate
Y220F
1500-fold increase in Km for D-glycerate
Y37A
significant increase in kcat, little effect on the Km value for GDP-Man